Technical Data Sheet
Ethidium bromide solution 0.5 % in dropper bottle, 75 ml, 5 x 15 ml
Ready-to-use products and reagents
Boiling point (bp) ~100 °C
Melting point (mp) ~0 °C
Store solution in the dark!
For staining of DNA/RNA after electrophoresis and for counterstaining of nuclei in histology and cytology.
€145.15/Pack Qty.
excl. VAT. | 75 ml per Pack Qty.
Art. No. HP46.2
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Product details
In spite of newly developed fluorescent staining reagents ethidium bromide remains the standard staining technic for nucleic acid in electrophoresis. Ethidium bromide is a carcinogenic substance which shouldn’t come in contact with skin. In order to minimise this danger we offer ready-to-use ethidium bromide solutions in dropper bottles for easy handling and maximum safety.
Excitation maxima (unbound): 210 nm, 285 nm, 470 nm
Excitation maxima (bound to DNA): 330 nm, 500 nm. Excitation possible via DNA at 254 nm
Emission maximum: 605 nm.
Ethidium bromide is a strong mutagenic and toxic substance. For your own safety, always wear gloves and respiratory protection to prevent contamination when working with this substance. After use, dispose of solutions correctly. They may not be discarded into the environment.
The following procedure is recommended (acc. to INSERM, Dossier prevention, Number 2, 2nd Ed., September 88):
1. Collect and dispose of as hazardous waste.
2. Break down EtBr with potassium permanganate and HCI (P. Quillardet, M. Hofnung, Trends Genet. 4,89 [1988]).
3. Break down EtBr with hypophosphoric acid and sodium nitrite (Lunn and Sansone; Analyt. Biochem. 162, 453-458 [1987]).
4. Absorption of EtBr with Amberlite® XAD-16 (Lunn and Sansone) or with activated carbon (Bensaude, O. Method, Trends Genet. 4, 89-90 [1988]) from the solution.
We recomment disposure by SEKUROKA®Decon-Bags (Art. No. T856)
Ethidium bromide solution 0.5 % in dropper bottle 5 mg/ml in water, ready-to-use
In order to prepare a staining solution add 5 drops of ethidium bromide solution to 500 ml water, buffer, or electrophoresis running buffer, respectively.
Nuclei / nucleic acids are stained red.
| Max. excitation (DNA-bound) |
330 nm 500 nm |
| Max. excitation (unbound) |
210 nm 285 nm 470 nm |
| Emissions maximum | 605 nm |
| Use | Addition to running buffer |
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| Art. No. | Pack Qty. | Pack. | Packaging | Price | Quantity | |
|---|---|---|---|---|---|---|
| HP46.1 | 15 ml | plastic | 1 x 15 ml |
€42.90 |
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| HP46.2 | 75 ml | plastic | 5 x 15 ml |
€145.15 |
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- Subtotal: 0.00
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General information
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The content of colourants is not specified for many products for various reasons:
1. complex composition: colourants are often mixtures of several components that may be present in different ratios. These compositions can vary, which makes it difficult to state an exact content.
2. application focus: In many applications, especially in the textile and food industries, the exact concentration of a dye is less relevant than its colouring power or its ability to achieve a certain colour. Here, the colour strength or the ability to impart colour is the most important criterion.
3. standardisation: Many dyes are traded according to certain standards (e.g. C.I. numbers) which guarantee a certain quality and colouring. These standards often make an exact specification of content superfluous, as users know that the colourant has the desired properties.
4. manufacturing processes: The manufacturing process of colourants can lead to natural variations in content. Instead of specifying an exact content, quality control is often carried out to ensure that the colourant is within an acceptable range.
5. Regulatory aspects: In some cases, regulatory requirements may not require the manufacturer to declare the exact content as long as the colourant is safe and effective for the intended purpose.
6. cost reasons: An exact determination of the content often requires complex analytical procedures that could increase production costs. Therefore, some manufacturers do not provide this additional information in order to save costs.
In summary, it can be said that the content of colourants is often not specified because other quality criteria are in the foreground and the exact concentration is not decisive for many applications.
Summary Staining of Nucleic Acid in Gels
| Product | Sensitivity | Description | Excitation |
| Ethidium bromide solution | 0.5-5 ng/band | Standard fluorescent staining | 254-330 nm, 500 nm UV- and blue light |
| SYBR® Green DNA dye | 0.01 ng/band | Non-toxic, green fluorescent staining | 254 nm and 495 nm UV- and blue light |
| ROTI®GelStain | 0.3 ng/band | Non-toxic, green fluorescent staining | 302 nm and 490 nm UV- and blue light |
| ROTI®GelStain Red | 0.3 ng/band | Non-toxic, red fluorescent staining | 310 nm, 540 nm UV- and blue light |
| ROTI®Methylene blue staining concentrate | 10 ng/band | Reversible, non-toxic blue staining | White light |
| ROTI®Black N | <0.1 ng/band | Silver staining of DNA polyacrylic amide gels | White light |