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ROTIPHORESE®10x SDS-PAGE, 1 l, glass

ROTIPHORESE® 10x conc.
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SDS-PAGE buffer, Tris-glycine-SDS buffer, Laemmli buffer
Density (D) 1,068 g/cm³
WGK 1

SDS-PAGE running buffer in electrophoresis.
Mix before use!

ROTIPHORESE®10x SDS-PAGE contains 0,25 M Tris, 1,92 M glycine and 1 % (w/v) SDS in distilled, deionised water for protein analysis.
Acc. to Laemmli (1970), Nature 227:680.
Product details
Composition

€40.75/Pack Qty. 

excl. VAT. | 1 l per Pack Qty.

Art. No. 3060.1

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Product details



ROTIPHORESE®10x SDS-PAGE ROTIPHORESE® 10x conc.

ROTIPHORESE®10x SDS-PAGE contains 0,25 M Tris, 1,92 M glycine and 1 % (w/v) SDS in distilled, deionised water for protein analysis.
Acc. to Laemmli (1970), Nature 227:680.


Directions for use

ROTIPHORESE®10x SDS-PAGE is usually used as 1x concentrated solution. SDS-PAGE buffer is suitable for the denaturing separation of proteins and peptides of any size in the discontinuous polyacrylamide gel (collection gel / separation gel).


Mechanism

SDS denatures proteins and binds in a constant mass ratio to their peptide chains, covering their intrinsic charge. In the collection gel (pH 6.8) all proteins are kept in a sharp band between the neutral Glycin and chloride ions and thus all reach the separation gel at the same time. After the pH change when the separation gel is reached (pH 8.8), the chloride ions and the now cationic Glycin migrate very quickly to the anode and leave the proteins to themselves, which now move in the electric field depending on size towards the anode.



ROTIPHORESE®10x SDS-PAGE
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3060.1 1 l glass €40.75
3060.2 5 l plastic €107.40
3060.3 2.5 l glass €74.10
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Composition

0.25 M TRIS, 1.92 M glycine., 1 % (g/v) SDS, distilled, deionized water., Remark: The product may crystallise. Can be redissolved by heating (max. 40 °C).