ROTIPHORESE®10x SDS-PAGE, 5 l, plastic
Mix before use!
€83.70/Pack Qty. Campaign price!
excl. VAT. | 5 l per Pack Qty.
Art. No. 3060.2
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Concentrated stock solution for tris/glycine/SDS gel run buffer (PAGE)
- Standardised composition according to Lämmli (Nature)
- Made from high-quality reagents
- For constant quality and highly reproducible gels
ROTIPHORESE®10x SDS-PAGE ROTIPHORESE® 10x conc.
ROTIPHORESE®10x SDS-PAGE contains 0,25 M Tris, 1,92 M glycine and 1 % (w/v) SDS in distilled, deionised water for protein analysis.
Acc. to Laemmli (1970), Nature 227:680.
- Standardised composition according to LämmliMade from high-quality reagentsFor constant quality and highly reproducible gels
ROTIPHORESE®10x SDS-PAGE is usually used as 1x concentrated solution. SDS-PAGE buffer is suitable for the denaturing separation of proteins and peptides of any size in the discontinuous polyacrylamide gel (collection gel / separation gel).
SDS denatures proteins and binds in a constant mass ratio to their peptide chains, covering their intrinsic charge. In the collection gel (pH 6.8) all proteins are kept in a sharp band between the neutral Glycin and chloride ions and thus all reach the separation gel at the same time. After the pH change when the separation gel is reached (pH 8.8), the chloride ions and the now cationic Glycin migrate very quickly to the anode and leave the proteins to themselves, which now move in the electric field depending on size towards the anode.
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|0.25 M TRIS, 1.92 M glycine., 1 % (g/v) SDS, distilled, deionized water., Remark: The product may crystallise. Can be redissolved by heating (max. 40 °C).|