ROTIPHORESE®10x TBE Buffer, 5 l, plastic
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Ready-to-use products and reagents
Boiling point (bp) >100 °C
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
WGK 1
Concentrated TBE tends to precipitate salts during storage. Mix before use!
Suitable for manufacturing sequencing gels with ROTIPHORESE Gel 40 and as a running buffer.
€93.45/Pack Qty.
excl. VAT. | 5 l per Pack Qty.
Art. No. 3061.2
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Product details
ROTIPHORESE®10x TBE Buffer ROTIPHORESE® 10x conc.
ROTIPHORESE®10x TBE buffer contains 1,0 M Tris-Borate pH 8,3 and 20 mM EDTA in distilled, deionised water; pH 8,3.
Suitable for manufacturing sequencing gels with ROTIPHORESE Gel 40 and as a running buffer.
ROTIPHORESE®TBE buffer is usually used as 1x or 0.5-fold concentrated solution. TBE buffer is particularly suitable for the separation of small DNA fragments in the gel (up to 3 kb). TBE buffer is not suitable for use of gels for gel extraction of DNA.
TBE buffer has a higher buffer capacity than TAE, but also leads to stronger heating of the gels. A slightly lower voltage should therefore be selected when gelling. DNA migrates in the TBE gel about half as fast as in TAE-buffered gels.
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| Order No. | Pack Qty. | Pack. | Price | Quantity | ||
|---|---|---|---|---|---|---|
| 3061.1 | 1 l | glass | €29.60 |
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| 3061.2 | 5 l | plastic | €93.45 |
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| 3061.3 | 2.5 l | glass | €60.10 |
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- Subtotal: 0.00
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Composition
| 1.0 M TRIS borate (pH 8.3), 20 mM EDTA., distilled, deionized water., Remark: The product may crystallise. Can be redissolved by heating (max. 40 °C). |