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ROTIPHORESE®10x TBE Buffer, 2.5 l, glass, 1 x 2.5 l

ROTIPHORESE® 10x conc.
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H360FD
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P201 P280 P308+P313
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Pack Qty.
Pack.
Packaging
TBE buffer
Density (D) 1,068 g/cm³
Boiling point (bp) >100 °C
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
WGK 1

TBE-buffer for preparing sequencing gels and as running buffer in electrophoresis.
Concentrated TBE tends to precipitate salts during storage. Mix before use!

The TBE buffer is optimally suited as a gel and running buffer system.
Product details

€76.25/Pack Qty. 

excl. VAT. | 2.5 l per Pack Qty.

Art. No. 3061.3

In stock
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from 6 Pack Qty. €72.44/Pack Qty.
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Product details


The TBE buffer is optimally suited as a gel and running buffer system.


Mechanism

TBE buffer has a higher buffer capacity than TAE, but also leads to stronger heating of the gels. A slightly lower voltage should therefore be selected when gelling. DNA migrates in the TBE gel about half as fast as in TAE-buffered gels.
ROTIPHORESE®TBE buffer has a high buffer capacity and is therefore ideally suited as a gel and running buffer system for sequencing gels.



ROTIPHORESE®10x TBE Buffer ROTIPHORESE® 10x conc.

ROTIPHORESE®10x TBE buffer contains 1,0 M Tris-Borate pH 8,3 and 20 mM EDTA in distilled, deionised water; pH 8,3.

Suitable for manufacturing sequencing gels with ROTIPHORESE®Gel 40 and as a running buffer.


Directions for use

ROTIPHORESE®TBE buffer is usually used as 1x or 0.5-fold concentrated solution. TBE buffer is particularly suitable for the separation of small DNA fragments in the gel (up to 3 kb). TBE buffer is not suitable for use of gels for gel extraction of DNA.



ROTIPHORESE®10x TBE Buffer
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Art. No. Pack Qty. Pack. Packaging Price Quantity
3061.1 1 l glass 1 x 1 l

€36.05

3061.2 5 l plastic 1 x 5 l

€119.90

3061.3 2.5 l glass 1 x 2.5 l

€76.25

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Certificates of Analysis

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Composition

1.0 M TRIS borate (pH 8.3), 20 mM EDTA., distilled, deionized water., Remark: The product may crystallise. Can be redissolved by heating (max. 40 °C).