Technical Data Sheet
ROTIPHORESE®NF 10x TBE Buffer
Ready-to-use products and reagents
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Boiling point (bp) 100 °C
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
WGK 1
Tested and optimised for automatic sequencers.
Concentrated TBE tends to precipitate salts during storage. Mix before use!
€131.70/Pack Qty.
excl. VAT. | 2.5 l per Pack Qty.
Art. No. A118.1
Fits to / Accessories
Product details
The TBE buffer is optimally suited as a gel and running buffer system.
TBE buffer has a higher buffer capacity than TAE, but also leads to stronger heating of the gels. A slightly lower voltage should therefore be selected when gelling. DNA migrates in the TBE gel about half as fast as in TAE-buffered gels.
ROTIPHORESE®TBE buffer has a high buffer capacity and is therefore ideally suited as a gel and running buffer system for sequencing gels.
ROTIPHORESE®NF 10x TBE Buffer ROTIPHORESE® 10x conc., fluorescence-free
10-times concentrated buffer formulated specially for automated sequencing.
10x stock solution contains 0,89 M Tris-Borate and 22 mM EDTA in distilled, deionised water; pH 8,3.
Suitable for manufacturing sequencing gels with ROTIPHORESE®NF Acrylamide/Bis-solution 40 % (19:1) and as running buffer.In sequencing, NF-TBE buffer is usually used in a final concentration of 1x.
Ideally suited as a gel and running buffer system and also for sequencing gels,
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| Art. No. | Pack Qty. | Pack. | Price | Quantity | |
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| A118.1 | 2.5 l | glass |
€131.70 |
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Available
In procurement
No longer available
Delivery date currently unknown
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Downloads / MSDS
Certificates of Analysis
Notes on the product
| Tested for automatic sequencers., 0.89 M TRIS borate (pH 8.3), 22 mM EDTA., distilled, deionized water., Remark: The product may crystallise. Can be redissolved by heating (max. 40 °C). |