Technical Data Sheet
ROTI®Load DNA (with glycerol)
Boiling point (bp) >100 °C
Storage temp. -20 °C
Transport temp. ambient temp.
Contains: Tris, Na-acetate, EDTA, bromophenol blue, xylene cyanol and glycerol
excl. VAT. | 9 ml per Pack Qty.
Art. No. X904.1
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The Carl ROTH gel loading buffers are special buffer mixtures for nucleic acid electrophoresis, which are used as sample additives during gel loading. The gel loading buffers are ready-to-use solutions, which may be used directly.
ROTI®Load DNA buffer solutions contain Tris, sodium acetate and EDTA, with additional tracking dyes. For increasing the density when loading the gel, they are available in three different variations - with glycerol, ficoll and saccharose.
ROTI®Load DNA can either be added directly to the dissolved sample or the precipitated RNA can be solubilised directly in ROTI®Load DNA. ROTI®Load DNA gel loading buffer is usually used as 1x concentrated solution.
ROTI®Load DNA (with glycerol) 6x conc., DNase-free
In order to cover the main size reange needed in gel electrophoresis, ROTI®Load DNA (with glycerol) contains bromophenol blue and xylene cyanol (see lane 1 in the product image).
Glycerin is the standard reagent for increasing sample density.
Each batch is tested for its functionality in electrophoresis and proved to be DNAse-free.
|Dye||Bromphenol blue, Xylene cyanole|
|Incl. DNA staining||no|
|Use||Standard applications, routine gels|
- Subtotal: 0.00
|Art. No.||Pack Qty.||Pack.||Packaging||Price||Quantity|
|X904.1||9 ml||plastic||5 x 1,8 ml||
No longer available
Delivery date currently unknown
Care should be taken to ensure that the dyes contained in the gel stop before smallest relevant DNA bands. This ensures that the buffer can be stopped in time. However, the selected dyes may overlay the bands shown. In this case, select a loading buffer that does not contain any unwanted dyes.
Bromophenol blue and xylene cyanol can be used as colour markers in all standard gels. If a relevant band is overlaid by one of these colour markers, choose a gel loading buffer containing Orange G. If small fragments are to be assayed, the gel loading buffer should contain Orange G in order to mark the run. If relevant bands are overlaid, however, a choice can be made between bromophenol blue or xylene cyanol depending on the size of the bands. A loading buffer without xylene cyanol is usually used for assaying large fragments.
Glycerine is the standard reagent for increasing the density of samples.
Ficoll® 400 produces particularly well-defined bands.
|Each batch is functionally tested for its suitability in electrophoresis and for DNAse-freeness.|