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ROTI®Load DNA-tricolor (with glycerol)

6x conc., DNase-free
Icon_ready-to-use Ready-to-use products and reagents
Icon_DNAse-free
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Density (D) 1 g/cm³
Storage temp. -20 °C
Transport temp. ambient temp.
WGK 1

DNA-gel loading buffer for DNA gel electrophoresis, suitable for a broad range of fragments.
Contains: Tris, Na-acetate, EDTA, bromophenol blue, xylene cyanol, orange G and glycerol

The Carl ROTH gel loading buffers are special buffer mixtures for nucleic acid electrophoresis, which are used as sample additives during gel loading. The gel loading buffers are ready-to-use solutions, which may be used directly.
Product details

€68.30/Pack Qty. 

excl. VAT. | 9 ml per Pack Qty.

Art. No. HP06.1

In production
from 6 Pack Qty. €64.88/Pack Qty.
from 24 Pack Qty. €61.47/Pack Qty.

Product details


The Carl ROTH gel loading buffers are special buffer mixtures for nucleic acid electrophoresis, which are used as sample additives during gel loading. The gel loading buffers are ready-to-use solutions, which may be used directly.


ROTI®Load DNA buffer solutions contain Tris, sodium acetate and EDTA, with additional tracking dyes. For increasing the density when loading the gel, they are available in three different variations - with glycerol, ficoll and saccharose.


Working concentration

ROTI®Load DNA can either be added directly to the dissolved sample or the precipitated RNA can be solubilised directly in ROTI®Load DNA. ROTI®Load DNA gel loading buffer is usually used as 1x concentrated solution.



ROTI®Load DNA-tricolor (with glycerol) 6x conc., DNase-free
Directions for use

ROTI®Load DNA-tricolor (with glycerol) is a gel loading buffer with orange G, bromophenol blue and xylencyanol as dyes (see lane 4 in the product image). Perfectly suited if a large fragment range is to be separated in the gel.


Glycerin is the standard reagent for increasing sample density.


Each batch is tested for its functionality in electrophoresis and proved to be DNAse-free.



Technical Information
Dye Bromphenol blue, Xylene cyanole, Orange G 
Incl. DNA staining no 
Use Broad size range, high flexibility 
ROTI®Load DNA-tricolor (with glycerol)
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Art. No. Pack Qty. Pack. Packaging Price Quantity
HP06.1 9 ml plastic 5 x 1,8 ml

€68.30

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Downloads / MSDS


General information

Selecting a Suitable ROTI®Load Gel Loading Buffer for Nucleic Acids

Care should be taken to ensure that the dyes contained in the gel stop before smallest relevant DNA bands. This ensures that the buffer can be stopped in time. However, the selected dyes may overlay the bands shown. In this case, select a loading buffer that does not contain any unwanted dyes.
Bromophenol blue and xylene cyanol can be used as colour markers in all standard gels. If a relevant band is overlaid by one of these colour markers, choose a gel loading buffer containing Orange G. If small fragments are to be assayed, the gel loading buffer should contain Orange G in order to mark the run. If relevant bands are overlaid, however, a choice can be made between bromophenol blue or xylene cyanol depending on the size of the bands. A loading buffer without xylene cyanol is usually used for assaying large fragments.

Glycerine is the standard reagent for increasing the density of samples.
Ficoll® 400 produces particularly well-defined bands.


Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Notes on the product

Each batch is functionally tested for its suitability in electrophoresis and for DNAse-freeness.