Technical Data Sheet
ROTI®Load DNAstain 3 SYBR® Green, 9.0 ml, 5 x 1.8 ml
Ready-to-use products and reagents
Storage temp. -20 °C
Transport temp. ambient temp.
WGK 1
Contains: Tris, EDTA, orange G, SYBR® Green.
1.8 ml are sufficient for gel run and staining of approx. 1,800 lanes (5 µl sample + 1 µl loading buffer).
€166.65/Pack Qty.
excl. VAT. | 9 ml per Pack Qty.
Art. No. 1CN7.2
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Product details
Loading bufer with the fluorescent dye SYBR® Green I for staining of double stranded nucleic acid in agarose and polyacrylamide gels.
- Alternative for ethidium bromide, non-toxic, non-mutagen
- 20 times more sensitive than ethidium bromide, up to 0.01 ng nucleic acid per band
- For use with common broad-band ethidium bromide photo filters
- Excitation possible via UV light (254 nm) and blue light (495 nm)
- Compatible with all usual down-stream applications.
ROTI®Load DNAstain SYBR® Green is easy to use and reduces significantly the time required for gel analysis. The contained fluorescent dye SYBR® Green I detects up to 0.01 ng per band, and is, therefore, 20 times more sensitive than ethidium bromide. DNA integrity is not influenced by SYBR® Green I; hence, gel eluted DNA may directly be applied to standard down-stream applications, like, e.g., ligation, PCR, transformation, transfection etc. without interfering effects.
SYBR® Green I bounds to the minor groove of DNA and intercalates into the DNA. It can be excited by UV- and blue light, making it fully compatible with standard UV-transilluminators as well as with modern blue-light illumination (see also ROTIPHORESE®PROfessional runVIEW, Art. No. 4849.1).
When excited, nucleic acid-bound SYBR® Green I emits a brightly coloured green fluorescence (521 nm) that may be documented by all usual broadband ethidium bromide- or SYBR® Green foto filters.
The figure depicts 50 ng DNA per band, or 75 ng (1000 bp and 2.5 kb), respectively. Each lane has been loaded and concurrently stained using 1 µl ROTI®Load DNAstain SYBR® Green.
Excitation maximum (bound to DNA): 254 nm and 495 nm
Emission maximum (bound to DNA): 521 nm
ROTI®Load DNAstain 3 SYBR® Green 6x conc., ready-to-use
ROTI®Load DNAstain 3 SYBR® Green is a special gel loading buffer with orange G as the only tracking dye optimized for very short runs. Orange G runs in the very small fragment range and shows the maximum running distance for very short runs. Also well suited if bromophenol blue or xylene cyanol could mask important DNA bands.
Glycerin is the standard reagent for increasing sample density.
Recommended for staining of dsDNA- and dsRNA-fragments of under 500 bp.
| Max. excitation (DNA-bound) |
~320 nm 470-500 nm |
| Amount of traces | ca. 1000/ml |
| Emissions maximum | 525 nm |
| Dye | Orange G, SYBR® Green |
| Incl. DNA staining | yes |
| Sensitivity | 0,01 ng/band |
| Use | Simultaneous gel staining, small fragments |
- Subtotal: 0.00
| Art. No. | Pack Qty. | Pack. | Packaging | Price | Quantity | |
|---|---|---|---|---|---|---|
| 1CN7.1 | 1.8 ml | plastic | 1 x 1.8 ml |
€53.65 |
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| 1CN7.2 | 9 ml | plastic | 5 x 1.8 ml |
€166.65 |
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In stock
Available
In procurement
No longer available
Delivery date currently unknown
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- Subtotal: 0.00
Downloads / MSDS
General information
Care should be taken to ensure that the dyes contained in the gel stop before smallest relevant DNA bands. This ensures that the buffer can be stopped in time. However, the selected dyes may overlay the bands shown. In this case, select a loading buffer that does not contain any unwanted dyes.
Bromophenol blue and xylene cyanol can be used as colour markers in all standard gels. If a relevant band is overlaid by one of these colour markers, choose a gel loading buffer containing Orange G. If small fragments are to be assayed, the gel loading buffer should contain Orange G in order to mark the run. If relevant bands are overlaid, however, a choice can be made between bromophenol blue or xylene cyanol depending on the size of the bands. A loading buffer without xylene cyanol is usually used for assaying large fragments.
Glycerine is the standard reagent for increasing the density of samples.
Ficoll® 400 produces particularly well-defined bands.
Summary Staining of Nucleic Acid in Gels
| Product | Sensitivity | Description | Excitation |
| Ethidium bromide solution | 0.5-5 ng/band | Standard fluorescent staining | 254-330 nm, 500 nm UV- and blue light |
| SYBR® Green DNA dye | 0.01 ng/band | Non-toxic, green fluorescent staining | 254 nm and 495 nm UV- and blue light |
| ROTI®GelStain | 0.3 ng/band | Non-toxic, green fluorescent staining | 302 nm and 490 nm UV- and blue light |
| ROTI®GelStain Red | 0.3 ng/band | Non-toxic, red fluorescent staining | 310 nm, 540 nm UV- and blue light |
| ROTI®Methylene blue staining concentrate | 10 ng/band | Reversible, non-toxic blue staining | White light |
| ROTI®Black N | <0.1 ng/band | Silver staining of DNA polyacrylic amide gels | White light |
Certificates of Analysis
Notes on the product
| Appearance | orange solution |
| Each batch is functionally tested for its suitability in electrophoresis. | |