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ROTI®Load 1, 10 ml, 1 x 10 ml

4x konz., reducing
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Icon_ready-to-use Ready-to-use products and reagents
Examples of effect: Damage metals and burn body tissues; may cause serious eye damage. Safety: Avoid contact; wear safety spectacles and gloves. In the event of contact with eyes and skin, rinse with water.
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Examples of effect: Are harmful to aquatic organisms, toxic or highly toxic, acute or with long-term effects. Safety: To be disposed of as hazardous waste only, do not allow into the environment under any circumstances.
Danger
H302+H332-H311-H315-H317-H318-H373-H411
i harmful if swallowed or if inhaled, toxic in contact with skin, causes skin irritation, may cause an allergic skin reaction, causes serious eye damage, may cause damage to organs through prolonged or repeated exposure, toxic to aquatic life with long lasting effects
P273 P280 P302+P352 P305+P351+P338 P310
i avoid release to the environment, wear protective gloves/eye protection, IF ON SKIN: Wash with plenty of water, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing, immediately call a POISON CENTER/doctor
Pack Qty.
Pack.
Packaging
Gel loading buffer, Laemmli buffer
Density (D) 1,2 g/cm³
Storage temp. +15 to +25 °C
ADR 6.1 III
WGK 3
UN-Nr. 2810

Protein gel loading buffer, modified formulation acc. to Laemmli.
Contains SDS (approx. 8 % w/v), β-mercapto ethanol (approx. 20 % v/v), glycerol (approx. 40 % v/v) and bromophenol blue (approx. 0,015 % w/v). Phosphate buffered.

ROTI®Load 1 (reducing) as well as ROTI®Load 2 and 3 (non-red ...
Product details
Type analysis
€18.85 /Pack Qty.

€13.15/Pack Qty.  Campaign price!

excl. VAT. | 10 ml per Pack Qty.

Art. No. K929.1

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Product details


ROTI®Load 1 (reducing) as well as ROTI®Load 2 and 3 (non-reducing) represent special gel loading buffers for protein gel electrophoresis. They have been designed to protect your proteins during sample preparation and to stabilise sensitive peptide bonds. Protein degradation is efficiently prevented by those buffer systems. ROTI®Load are 4x concentrated ready-to-use solutions, which may be used directly for sample loading.



ROTI®Load 1 4x konz., reducing

Well tested and proven sample buffer for protein gel electrophoresis that has been modified and further developed based on the original formulation acc. to Laemmli (Nature 227, 1970). Optimized for a distinct band display. Recommended for size estimation of proteins in gel.

  • Formulation acc. to Laemmli (modified)
  • Stabilises peptide bands
  • Prevents protein degradation
  • Improves gel separation

Directions for use

ROTI®Load protein gel loading buffers are usually used as 1x concentrated solution. ROTI®Load can either be added to the prepared sample or the precipitated proteins are dissolved directly into ROTI®Load. Heat the ROTI®Load mixed sample to 95 °C for 5 minutes to break hydrogen bonds and stretch the peptide chains. The β-Mercaptoethanol contained in ROTI®Load 1 opens disulfide bridges by reduction and leads to a complete molecular stretching, the basic requirement for a correct size estimation of proteins in the gel.


ROTI®Load 1 prevents degradation of proteins while heating during sample preparation, additionally keeping the sample pH value constant during gel electrophoresis. Being a phosphate-buffered ready-to-use solution, ROTI®Load 1 already contains all necessary denaturing and reducing reagents, bromophenol blue as dye and glycerol as density increasing reagent.



ROTI®Load 1
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Order No. Pack Qty. Pack. Packaging Price Quantity
K929.1 10 ml glass 1 x 10 ml €13.15
K929.2 40 ml glass 4 x 10 ml €38.55
K929.3 100 ml glass 10 x 10 ml €89.15
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Downloads


Analysis certificates

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Type analysis

Appearancedark blue, clear solution  
pH value (1x sol.)6,6-7,2  
Remark: Contains SDS. Can be redissolved by heating (max. 45 °C).

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