Technical Data Sheet
ROTI®Load 1, 10 ml, 1 x 10 ml
Storage temp. +15 to +25 °C
ADR 6.1 III
Contains SDS (approx. 8 % w/v), β-mercapto ethanol (approx. 20 % v/v), glycerol (approx. 40 % v/v) and bromophenol blue (approx. 0,015 % w/v). Phosphate buffered.
€17.60/Pack Qty. Campaign price!
excl. VAT. | 10 ml per Pack Qty.
Art. No. K929.1
- Subtotal: 0.00
|Art. No.||Pack Qty.||Pack.||Packaging||Price||Quantity|
|K929.1||10 ml||glass||1 x 10 ml||
|K929.2||40 ml||glass||4 x 10 ml||
|K929.3||100 ml||glass||10 x 10 ml||
No longer available
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ROTI®Load 1 is suitable for all PAGE standard gels. It has denaturing and reducing properties.
ROTI®Load 2 is suitable for protein complexes, secondary structures and native gels. It has denaturing properties, but it does not have reducing properties. It is possible to enrich the buffer solution with reducing agents such as β-Mercaptoethanol or DTT.
ROTI®Load 3 is suitable for native gels, precast gels and cooled gels. The buffer solution contains LDS. Unlike SDS, LDS does not crystallise out at low temperatures. In addition, the buffer solution is optimally adapted for precast gels. ROTI®Load 3 has denaturing properties, but does not have reducing properties. It is possible to enrich the buffer solution with reducing agents such as ß-Mercaptoethanol or DTT.
Glycerine is the standard reagent for increasing the density of samples.
SDS (sodium dodecyl sulphate), LDS (lithium dodecyl sulphate) are anionic surfactants. They form a negatively charged mantle around the protein molecules, which results in a denaturing effect. This process is reversible. Irreversible denaturation only occurs if the samples are boiled. Negative charging allows the molecules to be separated during electrophoresis.
β-mercaptoethanol, DTT (dithiothreitol) are reducing agents which split disulphide bridges in protein molecules. Increased unfolding of the molecule occurs. This process is reversible.
|Appearance||dark blue, clear solution|
|pH value (1x sol.)||6.6-7.2|
|Remark: Contains SDS. Can be redissolved by heating (max. 45 °C).|