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ROTI®Load 1, 10 ml, 1 x 10 ml

4x conc., reducing
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i harmful if swallowed or if inhaled, toxic in contact with skin, causes skin irritation, may cause an allergic skin reaction, causes serious eye damage, may cause damage to organs (liver, heart) through prolonged or repeated exposure (if swallowed), toxic to aquatic life with long lasting effects
P273 P280 P302+P352 P305+P351+P338 P310
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Pack Qty.
Gel loading buffer, Laemmli buffer
Density (D) 1,2 g/cm³
Storage temp. +15 to +25 °C
UN-Nr. 2810

Protein gel loading buffer, modified formulation acc. to Laemmli.
Contains SDS (approx. 8 % w/v), β-mercapto ethanol (approx. 20 % v/v), glycerol (approx. 40 % v/v) and bromophenol blue (approx. 0,015 % w/v). Phosphate buffered.

€22.05 /Pack Qty.

€17.60/Pack Qty.  Campaign price!

excl. VAT. | 10 ml per Pack Qty.

Art. No. K929.1

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ROTI®Load 1
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Art. No. Pack Qty. Pack. Packaging Price Quantity
K929.1 10 ml glass 1 x 10 ml


K929.2 40 ml glass 4 x 10 ml


K929.3 100 ml glass 10 x 10 ml


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Downloads / MSDS

General information

Selecting a Suitable ROTI®Load Gel Loading Buffer for Proteins

ROTI®Load 1 is suitable for all PAGE standard gels. It has denaturing and reducing properties.
ROTI®Load 2 is suitable for protein complexes, secondary structures and native gels. It has denaturing properties, but it does not have reducing properties. It is possible to enrich the buffer solution with reducing agents such as β-Mercaptoethanol or DTT.
ROTI®Load 3 is suitable for native gels, precast gels and cooled gels. The buffer solution contains LDS. Unlike SDS, LDS does not crystallise out at low temperatures. In addition, the buffer solution is optimally adapted for precast gels. ROTI®Load 3 has denaturing properties, but does not have reducing properties. It is possible to enrich the buffer solution with reducing agents such as ß-Mercaptoethanol or DTT.

Glycerine is the standard reagent for increasing the density of samples.
SDS (sodium dodecyl sulphate), LDS (lithium dodecyl sulphate) are anionic surfactants. They form a negatively charged mantle around the protein molecules, which results in a denaturing effect. This process is reversible. Irreversible denaturation only occurs if the samples are boiled. Negative charging allows the molecules to be separated during electrophoresis.
β-mercaptoethanol, DTT (dithiothreitol) are reducing agents which split disulphide bridges in protein molecules. Increased unfolding of the molecule occurs. This process is reversible.

Certificates of Analysis

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The following analysis certificates have been found:

Type analysis

Appearancedark blue, clear solution
pH value (1x sol.)6.6-7.2
Remark: Contains SDS. Can be redissolved by heating (max. 45 °C).