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ROTI®Load 3 (LDS)

4x konz., unreducing
Icon_ready-to-use Ready-to-use products and reagents
Examples of effect: Lead to damage to heath, cause irritation to eyes, skin or respiratory organs. Are fatal in larger quantities. Safety: As previously stated, in the event of skin irritation or contact with eyes, rinse with water or a suitable medium.
Warning
H315-H319
i causes skin irritation, causes serious eye irritation
P280 P302+P352 P305+P351+P338 P337+P313
i wear protective gloves/protective clothing/eye protection/face protection, IF ON SKIN: Wash with plenty of water/..., IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing, if eye irritation persists: Get medical advice/attention
Pack Qty.
Pack.
Gel loading buffer, Laemmli buffer
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
WGK 3

Protein gel loading buffer for ready-made gels and cooled gels.
Contains LDS (approx. 4 % w/v), glycerol (approx. 40 % v/v) and Ficoll® 400 (approx. 4 % w/v), plus phenol red (approx. 0,025 % w/v) and Coomassie® G250 (approx. 0,025 % w/v). Triethanolamine buffered.

ROTI®Load 1 (reducing) as well as ROTI®Load 2 and 3 (non-red ...
Product details
Type analysis

€21.40/Pack Qty. 

excl. VAT. | 10 ml per Pack Qty.

Art. No. 3359.1

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Product details


ROTI®Load 1 (reducing) as well as ROTI®Load 2 and 3 (non-reducing) represent special gel loading buffers for protein gel electrophoresis. They have been designed to protect your proteins during sample preparation and to stabilise sensitive peptide bonds. Protein degradation is efficiently prevented by those buffer systems. ROTI®Load are 4x concentrated ready-to-use solutions, which may be used directly for sample loading.



ROTI®Load 3 (LDS) 4x konz., unreducing

Modified, non-reducing protein sample-gel loading buffer for gels under denaturing or native conditions.

  • Optimised for ready-made gels and cooled gel runs
  • Superior solubility at low temperatures
  • Stabilises proteins in the gel

Directions for use

ROTI®Load protein gel loading buffers are usually used as 1x concentrated solution. ROTI®Load can either be added to the prepared sample or the precipitated proteins are dissolved directly into ROTI®Load. Optionally, heat the ROTI®Load mixed sample to 95 °C for 5 minutes to break hydrogen bonds and stretch the peptide chains.


During sample preparation and heating, ROTI®Load 3 (LDS) efficiently prevents degradation of your proteins, also stabilising the pH value during the run.
This gel loading buffer is based on a triethanolamine buffered ready-to-use solution, in which the standard sodium dodecylsulphate (SDS) has been replaced by lithium dodecylsulphate (LDS). At low temperatures, LDS is much better soluble than SDS. Hence, no crystallisation of LDS takes place, and protein stability as well as band resolution is significantly enhanced.
Additionally, the formulation of ROTI®Load 3 (LDS) has been optimised for ready-made gels, particularly ROTI®PAGE gels, but also ready-made gels of other standard suppliers.

For reducing conditions, ROTI®Load 3 (LDS) may be supplemented with 20 µl/ml DTT solution (1 M) or with β-mercaptoethanol.



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Analysis certificates

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The following analysis certificates have been found:

Type analysis

Appearancegreen-blue, clear solution  
pH value (1x sol.)7,7 ±0,1  

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