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HEPES, 500 g

≥99,5 %, BioScience Grade
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N-2-Hydroxyethylpiperazine-N'-2-ethane sulphonic acid, Good's buffer
Empirical formula C8H18N2O4S
Molar mass (M) 238,31 g/mol
Flash point (flp) 124 °C
Melting point (mp) 212,6 °C
CAS No. 7365-45-9
EG-Nr. 230-907-9

For molecular biology and biotechnology.

€181.70/Pack Qty. 

excl. VAT. | 500 g per Pack Qty.

Art. No. 6763.2

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Product details

Free acid

HEPES ≥99,5 %, BioScience Grade

HEPES is a versatile buffer, which is zwitterionic over a broad pH-range, interesting for biological assays, and, therefore, doesn’t pass through biological membranes. In contrast to NaHCO3 buffers, it also doesn’t release any CO2 which affects the surroundings and has to be compensated. Hence, HEPES is particularly adapted for cell- and tissue cultures.

HEPES cannot be autoclaved and reacts with DEPC. Thus it's recommended to use DNase/RNase/Protease free HEPES BioScience-Grade as soon as molecular biological applications like PCR, extraction, isolation, hybridization etc. are to be performed.

Buffer reagent according to Good. High buffer capacity at physiological pH-values.

Technical Information
pH range 6.8 to 8.2 
Buffer reagent N-2-Hydroxyethyl piperazine-N'-2-ethane sulphonic acid, Good's buffer 
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Art. No. Pack Qty. Pack. Price Quantity
6763.1 100 g plastic


6763.2 500 g plastic


6763.3 1 kg plastic


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Downloads / MSDS

Certificates of Analysis

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Guarantee analysis

Assay≥99,5 %
Solubility (0,1 M in H2O)clear, complete
pKa (at 25 °C)7,45-7,65
260 nm≤0,02
280 nm≤0,02
405 nm≤0,01
Chloride (Cl)≤0,005 %
Sulphate (SO4)≤0,01 %
Phosphate (PO4)≤0,001 %
Calcium (Ca)≤0,003 %
Sodium (Na)≤0,005 %
Heavy metals (as Pb)≤0,0005 %
DNases, RNases, Proteasenone detected