HEPES, 500 g
Molar mass (M) 238,31 g/mol
Flash point (flp) 124 °C
Melting point (mp) 212,6 °C
WGK 1
CAS No. 7365-45-9
EG-Nr. 230-907-9
€181.70/Pack Qty.
excl. VAT. | 500 g per Pack Qty.
Art. No. 6763.2
Alternative products
Product details
Free acid
HEPES ≥99,5 %, BioScience Grade
HEPES is a versatile buffer, which is zwitterionic over a broad pH-range, interesting for biological assays, and, therefore, doesn’t pass through biological membranes. In contrast to NaHCO3 buffers, it also doesn’t release any CO2 which affects the surroundings and has to be compensated. Hence, HEPES is particularly adapted for cell- and tissue cultures.
HEPES cannot be autoclaved and reacts with DEPC. Thus it's recommended to use DNase/RNase/Protease free HEPES BioScience-Grade as soon as molecular biological applications like PCR, extraction, isolation, hybridization etc. are to be performed.
Buffer reagent according to Good. High buffer capacity at physiological pH-values.
| pH range | 6.8 to 8.2 |
| Buffer reagent | N-2-Hydroxyethyl piperazine-N'-2-ethane sulphonic acid, Good's buffer |
- Subtotal: 0.00
| Art. No. | Pack Qty. | Pack. | Price | Quantity | |
|---|---|---|---|---|---|
| 6763.1 | 100 g | plastic |
€46.15 |
|
|
| 6763.2 | 500 g | plastic |
€181.70 |
|
|
| 6763.3 | 1 kg | plastic |
€310.70 |
|
|
|
In stock
In procurement
No longer available
Delivery date currently unknown
|
|||||
- Subtotal: 0.00
Downloads / MSDS
Certificates of Analysis
Guarantee analysis
| Assay | ≥99,5 % |
| Solubility (0,1 M in H2O) | clear, complete |
| pKa (at 25 °C) | 7,45-7,65 |
| Absorbance | |
| 260 nm | ≤0,02 |
| 280 nm | ≤0,02 |
| 405 nm | ≤0,01 |
| Chloride (Cl) | ≤0,005 % |
| Sulphate (SO4) | ≤0,01 % |
| Phosphate (PO4) | ≤0,001 % |
| Calcium (Ca) | ≤0,003 % |
| Sodium (Na) | ≤0,005 % |
| Heavy metals (as Pb) | ≤0,0005 % |
| DNases, RNases, Protease | none detected |