Technical Data Sheet
HPLC column NUCLEODUR® 300-5 C4 ec, 250 mm, 4 mm
€462.25/Pack Qty.
excl. VAT. | 1 unit(s) per Pack Qty.
Art. No. 1HKK.1
Product details
Wide pore RP phase for daily routine analysis. The medium density butyl modification is based on high purity NUCLEODUR® silica gel and is often used for the separation of biomolecules.
Butyl (C4) modifications have a lower retention for hydrophobic compounds compared to C18 (octadecyl silane, ODS) or C8 (octyl, MOS) modifications.
- Butyl phase, endcapped
- Carbon content: 2.5 %
- Pore size: 300 Å
- pH stability: 1-9
- Eluent on column: acetonitrile/water
Similar phases:
Jupiter® C4; ACE® C4.
Further attractive products to complete your chromatography laboratory can be found on our Chromatography page!
For protection of your main HPLC column from contamination, it is recommended to use guard columns. These short columns are placed into a guard column holder. The guard column holder in combination with a guard column inside is installed between the injector and the HPLC column. This installation prevents chemical impurities to reach the main column. Because a contamination could form an irreversible bond with the column packing material (stationary phase) and this can result in shorter column life. If you want to prolong the lifetime of your main column, then these short columns can help you.
Further HPLC columns from Macherey-Nagel available on request.
Non-polar phases for routine analysis
Standard RP phase (110 Å) for daily routine analysis and up-scaling to preparative HPLC. Also available wide-pore (300 Å) for separation of biomolecules in analytical columns.
Properties:
- Cost-effective and reliable
- Long-life columns
- For all standard routine applications in modern RP-chromatography
Recommended application(s):
- Pore size 100 Å:
Basic, neutral and acidic pharmaceuticals, derivatised amino acids, pesticides, fat-soluble vitamins, aldehydes and ketones, phenolic compounds. - Pore size 300 Å:
Biological macromolecules, such as proteins or peptides. - USP L1 (C18) · USP L7 (C8) · USP L26 (C4)
HPLC column NUCLEODUR® 300-5 C4 ec
| Particle size | 5 µm |
| Column length | 250 mm |
| Column inner diameter | 4 mm |
| Column volume | 3.15 ml |
| Endcapped | yes |
| Eluent in column | Acetonitrile - Water |
| Phase | 300-5 C4 ec |
| Surface chemistry | Medium density monomeric butyl (C4) wide-pore modification |
| USP listing | L26 |
| Separation principle | Reversed phase (RP) |
| Column shape | Analytical separation column |
| Column type | HPLC column |
| Particle type | Fully porous particles (FPP) |
| Specific surface according to BET | 100 m²/g |
| Pore volume | 0.9 ml/g |
| Pore size | 300 Å |
| Temperature stability | 60 °C |
| Pressure stability | 450 bar |
| pH stability | 1,0-9,0 |
| Recommended application(s) | Biological macromolecules, Peptides, Proteins |
| Similar phases |
Jupiter® C4 ACE® C4 |
- Subtotal: 0.00
| Art. No. | Particle size | Column length | Column inner diameter | Pack Qty. | Price | Quantity | |
|---|---|---|---|---|---|---|---|
| 1HKK.1 | 5 µm | 250 mm | 4 mm | 1 unit(s) |
€462.25 |
|
|
| 1HKL.1 | 5 µm | 250 mm | 4,6 mm | 1 unit(s) |
€488.05 |
|
|
| 1HKE.1 | 5 µm | 150 mm | 4 mm | 1 unit(s) |
€379.50 |
|
|
| 1HKH.1 | 5 µm | 150 mm | 4,6 mm | 1 unit(s) |
€401.00 |
|
|
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- Subtotal: 0.00
Downloads / MSDS
General information
Further attractive products to complete your chromatography laboratory can be found on our Chromatography page!
NUCLEODUR® as a state-of-the-art silica is the ideal base material for modern HPLC phases. We offer two phases NUCLEODUR® Gravity which is ideal for sophisticated analytical separations and NUCLEODUR® ec suitable for routine HPLC analysis.