Technical Data Sheet
HPLC column NUCLEODUR® C8 Gravity 5 µm, 250 mm, 4 mm
€747.15/Pack Qty.
excl. VAT. | 1 unit(s) per Pack Qty.
Art. No. 1HLE.1
Product details
Reduced retention of non-polar compounds.
- Octyl phase, multi-endcapped
- Carbon content: 11.0 %
- Pore size: 110 Å
- pH stability: 1-11
- Eluent on column: acetonitrile/water
Applications:
analgesics, antidepressants, antiinflammatory drugs, herbicides, immunosuppressants, pharmaceuticals, phytopharmaceuticals, USP L7, suitable for LC/MS.
Similar phases:
NUCLEOSIL® C8 HD; Xterra® RP8 / MS C8; Luna® C8; Zorbax® Eclipse XDB-C8.
Further attractive products to complete your chromatography laboratory can be found on our Chromatography page!
For protection of your main HPLC column from contamination, it is recommended to use guard columns. These short columns are placed into a guard column holder. The guard column holder in combination with a guard column inside is installed between the injector and the HPLC column. This installation prevents chemical impurities to reach the main column. Because a contamination could form an irreversible bond with the column packing material (stationary phase) and this can result in shorter column life. If you want to prolong the lifetime of your main column, then these short columns can help you.
Further HPLC columns from Macherey-Nagel available on request.
The ideal phase for overall sophisticated analytical separations
This high density phase with its endcapping suppresses any unwanted polar interactions between the silica surface and the sample, which makes “Gravity” particularly suitable for the separation of basic and other ionizable analytes.
Properties:
- Suitable for LC/MS and HPLC at pH extremes (pH 1-11)
- Ideal for method development
- Superior base deactivation
- Multi-Endcapping
Recommended application(s):
- In general, compounds with ionisable functional groups such as basic pharmaceuticals and pesticides e.g. analgesics, anti-inflammatories, antidepressants, herbicides, phytopharmaceuticals, immunosuppressants.
- USP L1 (C18) · USP L7 (C8)
HPLC column NUCLEODUR® C8 Gravity 5 µm
| Particle size | 5 µm |
| Column length | 250 mm |
| Column inner diameter | 4 mm |
| Column volume | 3.15 ml |
| Endcapped | yes |
| Eluent in column | Acetonitrile - Water |
| Phase | C8 Gravity |
| Surface chemistry | Base deactivated (BDS), High density monomeric octyl (C8, MOS) modification |
| USP listing | L7 |
| Separation principle | Reversed phase (RP) |
| Column shape | Analytical separation column |
| Column type | HPLC column |
| Particle type | Fully porous particles (FPP) |
| Specific surface according to BET | 340 m²/g |
| Pore volume | 0.9 ml/g |
| Pore size | 110 Å |
| Temperature stability | 60 ° C |
| Pressure stability | 600 bar |
| pH stability | 1,0-11,0 |
| Recommended application(s) |
Analgesics, Antidepressants, Antiinflammatory drugs, Herbicides, Immunosuppressants, Pharmaceuticals, Phytopharmaceuticals Applications at pH extremes (1-11) Ideal for method development |
| Similar phases |
NUCLEOSIL® C8 HD Xterra® RP8 / MS C8 Luna® C8 Zorbax® Eclipse XDB-C8 |
- Subtotal: 0.00
| Art. No. | Particle size | Column length | Column inner diameter | Pack Qty. | Price | Quantity | |
|---|---|---|---|---|---|---|---|
| 1HLA.1 | 5 µm | 150 mm | 4 mm | 1 unit(s) |
€655.75 |
|
|
| 1HLC.1 | 5 µm | 150 mm | 4,6 mm | 1 unit(s) |
€720.25 |
|
|
| 1HLE.1 | 5 µm | 250 mm | 4 mm | 1 unit(s) |
€747.15 |
|
|
| 1HLH.1 | 5 µm | 250 mm | 4,6 mm | 1 unit(s) |
€790.15 |
|
|
|
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- Subtotal: 0.00
Downloads / MSDS
General information
Further attractive products to complete your chromatography laboratory can be found on our Chromatography page!
NUCLEODUR® as a state-of-the-art silica is the ideal base material for modern HPLC phases. We offer two phases NUCLEODUR® Gravity which is ideal for sophisticated analytical separations and NUCLEODUR® ec suitable for routine HPLC analysis.