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Phenylmethyl sulphonyl fluoride, 100 g, plastic

≥98 %, for biochemistry
Icon_EVE-EUD Please consider that this product might be liable to some legal sanctions as for example to obtain an end user declaration or the prohibition of some applications. For details please do contact your counterpart in our sales department!
Examples of effect: Damage metals and burn body tissues; may cause serious eye damage. Safety: Avoid contact; wear safety spectacles and gloves. In the event of contact with eyes and skin, rinse with water.
Examples of effect: In smaller quantities, lead to immediate serious damage to health or death. Safety: Do not inhale, touch or swallow. Wear work safety equipment. Call a poison control centre or doctor immediately. Recovery position.
Danger
H301-H314
i toxic if swallowed, causes severe skin burns and eye damage
P280 P301+P330+P331 P303+P361+P353 P305+P351+P338 P310
i wear protective gloves/protective clothing/eye protection/face protection, IF SWALLOWED: rinse mouth. Do NOT induce vomiting, IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water [or shower], IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing, immediately call a POISON CENTER/doctor
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Pack.
α-Toluene sulphonyl fluoride, PMSF
Empirical formula C7H7FO2S
Molar mass (M) 174,2 g/mol
Density (D) 0,79 g/cm³
Melting point (mp) 94 °C
ADR 8 II
WGK 1
CAS No. [329-98-6]
EG-Nr. 206-350-2
UN-Nr. 2923

Irreversible inhibitor of serine- and thiol proteases. Reversible effect on cysteine proteases.
Stock solution: 17.4 mg/ml (100 mM) in isopropanol, ethanol or methanol.
Storage of stock solution protected from light at -20 °C.

For your convenience, Art. No. 6367.4 comes packed in a 30 ml brown glass bottle. Addition of 20 ml 2-propanol results in a 100 mM stock solution.

General, irreversible inhibitor of serin proteases (chymotrypsin, trypsin and thrombin) and for the dystin-protease papain. Inhibiton by sulphonation of the serine localised in the enzymatic active site.

Reversible inhibitor of cysteine-proteases. Ki = 0.91 ±0.01 mM
Product details

€309.10/Pack Qty. 

excl. VAT. | 100 g per Pack Qty.

Art. No. 6367.3

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Product details



Phenylmethyl sulphonyl fluoride ≥98 %, for biochemistry

General, irreversible inhibitor of serin proteases (chymotrypsin, trypsin and thrombin) and for the dystin-protease papain. Inhibiton by sulphonation of the serine localised in the enzymatic active site.

Reversible inhibitor of cysteine-proteases. Ki = 0.91 ±0.01 mM


Directions for use

Working concentration: 17-174 µg/ml (0,1-1 mM).
Dissolves easily in isopropanol, ethanol and methanol. Not stable in aqueous solutions (half-life value of 1 h at pH 7.5/RT).
For stock solutions of 200-250 mM heat shortly (approx. 30 mins) to 30 °C.
Stock solutions should be stored at -20 °C. Short term storage of few weeks may be done at +4 °C in the dark.
PMSF reacts with thiols. DTT and β-mercaptoethanol reduce the inhibitory effect.
At every stage of isolating or purifying the proteins, PMSF should be added fresh from the stock solution. PMSF is often not sufficient as the only protease inhibitor in the preparation to protect against decomposition processes, as for example high salt concentration (3.0 M) impairs the inhibitory effect of PMSF.



Phenylmethyl sulphonyl fluoride
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Order No. Pack Qty. Pack. Price Quantity
6367.1 5 g plastic €33.25
6367.2 25 g plastic €110.65
6367.3 100 g plastic €309.10
6367.4 348 mg glass €15.60
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Downloads / MSDS


Certificates of Analysis

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The following analysis certificates have been found:

Type analysis

AppearanceWhite to beige powder or crystals
Assay≥98 %
Identity (Raman)complies
Melting point91-95 °C
Solubility in ethanolcomplies
Solubility in methanolcomplies

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