Technical Data Sheet
ROTI®Cell Hybridoma Medium
Sterile products
Ready-to-use products and reagents
Boiling point (bp) ~100 °C
Storage temp. +2 to +8 °C
Transport temp. ambient temp.
The ROTI®Cell media and supplements for hybridoma cell cultures are suitable for nearly all systems and provide optimal conditions for the development and production of various antibodies.
€68.70/Pack Qty.
excl. VAT. | 500 ml per Pack Qty.
Art. No. 3AA2.1
Product details
Hybridoma cells are cells that result from the fusion of B lymphocytes and myeloma cells. The outcome is immortal cells capable of producing novel antibodies.
The ROTI®Cell media and supplements for hybridoma cell cultures are suitable for nearly all systems and provide optimal conditions for the development and production of various antibodies.
ROTI®Cell Hybridoma Medium CELLPURE® sterile, w/o glutamine, w/o phenol red
ROTI®Cell Hybridoma medium is a serum-free medium that is free from animal-derived components, proteins, and any factors that could interfere with the downstream process and the purity of the final product.
The medium supports the growth of hybridoma and myeloma cells for antibody production. It can be used both as a serum-free medium and supplemented with serum.
In the case of cholesterol-dependent cell lines, the ROTI®Cell Hybridoma Supplement should additionally be used.
The medium does not contain: L-glutamine, phenol red, insulin, transferrin.
| Instructions for use, addition | Store at +2 °C to +8 °C protected from light. Once opened, use within 6-8 weeks. |
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| Art. No. | Pack Qty. | Pack. | Price | Quantity | |
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| 3AA2.1 | 500 ml | plastic |
€68.70 |
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Available (note delivery time)
In procurement
No longer available
Delivery date currently unknown
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Downloads / MSDS
General information
In addition to the various nutrients in the medium, the pH value also plays a very important role in cell culture. The physiological pH for almost all mammalian cells is 7.4. Deviating from this, it has been observed that certain transformed cell lines prefer a pH between 7.0 and 7.4 and certain fibroblasts prefer a pH between 7.4 and 7.7. In any case, pH fluctuations should be avoided and the pH should be maintained as well as possible. That is why a buffer system in media is necessary. The most important buffer system in the body is the carbonic acid-carbonate buffer system. Accordingly, almost all cell culture media contain bicarbonate (NaHCO3). Bicarbonate alone leads to a strong pH increase of the medium, so that NaHCO3-buffered media must always be used with CO2 incubators. Depending on the NaHCO3 concentration in the medium, different CO2 environments are necessary to ensure a pH of about 7.4. For this reason, you should not work outside of the CO2 incubator for too long. If this is necessary, a HEPES buffer should be used in addition to the NaHCO3, which contributes as a second buffer system to stabilize the pH value.
| Medium | Art. No. | Glucose g/l |
Glutamine (mM) |
HEPES (mM) |
Sodium Pyruvate (mM) |
NaHCO3 (mM) |
Phenol Red |
Salts | Note |
| Dulbecco’s Modified Eagle Media | |||||||||
| ROTI®Cell DMEM High Glucose | 9005.1 | 4.5 | 4 | X | 1 | 44 | yes | X | |
| ROTI®Cell DMEM High Glucose | 9006.1 | 4.5 | 4 | X | X | 44 | yes | X | |
| ROTI®Cell DMEM High Glucose | 9007.1 | 4.5 | 4 stab. | X | 1 | 44 | yes | X | With temperature stabile glutamine. |
| ROTI®Cell DMEM High Glucose | 1TE9.1 | 4,5 | 4 stab. | 25 | X | 44 | yes | X | With temperature stabile glutamine. |
| ROTI®Cell DMEM High Glucose | 9010.1 | 4.5 | X | X | 1 | 44 | yes | X | |
| ROTI®Cell DMEM High Glucose | 9019.1 | 4.5 | X | X | X | 44 | yes | X | |
| ROTI®Cell DMEM High Glucose | 1TE8.1 | 4.5 | X | X | X | 44 | X | X | Without phenol red. |
| ROTI®Cell DMEM Low Glucose | 1TE7.1 | 1.0 | 4 stab. | X | 1 | 44 | yes | With temperature stabile glutamine. | |
| ROTI®Cell DMEM Low Glucose | 9027.1 | 1.0 | X | X | 1 | 44 | yes | X | |
| DMEM : F12 | |||||||||
| ROTI®Cell DMEM : F12 | 1TE5.1 | 3.151 | X | 15 | 0,5 | 14.3 | yes | X | |
| ROTI®Cell DMEM : F12 | 1YTP.1 | 3.151 | 2.5 | 15 | 0,5 | 14.3 | yes | X | |
| Iscove’s Modfied Dulbecco Media | |||||||||
| ROTI®Cell Iscove’s MDM | 9033.1 | 4.5 | 4 | 25 | 1 | 36 | yes | X | Additional amino acids and selene. |
| Minimum Essential Media | |||||||||
| ROTI®Cell Eagle’s MEM / Earle’s | 9044.1 | 1 | 2 | X | X | 26.2 | yes | Earle’s | |
| ROTI®Cell Eagle’s MEM / Earle’s | 1TE6.1 | 1 | 2 stab. | X | X | 26.2 | yes | Earle’s | With temperature stabile glutamine. |
| ROTI®Cell Eagle’s MEM / Earle’s | 9047.1 | 1 | X | X | X | 26.2 | yes | Earle’s | |
| ROTI®Cell Eagle’s MEM-Alpha | 9058.1 | 1 | X | X | 1 | 26.2 | yes | Earle’s | Plus cobalamin, ascorbic acid, NEAA*, lipoic acid, biotin. |
| ROTI®Cell Eagle’s MEM-Alpha | 1TEA.1 | 1 | X | X | 1 | 26.2 | yes | Earle’s | Plus cobalamin, ascorbic acid, NEAA*, lipoic acid, biotin, ribonucleosides and desoxyribonucleosides. |
| RPMI Media | |||||||||
| ROTI®Cell RPMI 1640 | 9085.1 | 2 | 2 | X | X | 23.8 | yes | X | |
| ROTI®Cell RPMI 1640 | 9086.1 | 2 | 2 | 25 | X | 23.8 | yes | X | |
| ROTI®Cell RPMI 1640 | 9091.1 | 2 | 2 stab. | X | X | 23.8 | yes | X | With temperature stabile glutamine. |
| ROTI®Cell RPMI 1640 | 9099.1 | 2 | X | X | X | 23.8 | yes | X | |
| ROTI®Cell RPMI 1640 | 9104.1 | 2 | X | X | X | 23.8 | X | X | |
| Special Media | |||||||||
| ROTI®Cell Ham’s F12 | 9108.1 | 1.8 | 1 | X | 1 | 14 | yes | X | |
| ROTI®Cell Leibovitz’s L15 | 9109.1 | 0.9 galact. | X | X | 5 | X | yes | X | With galactose instead of glucose. Without sodium bicarbonate. |
| ROTI®Cell McCoy’s 5A | 9111.1 | 3 | 1.5 | X | X | 26.2 | yes | X | |
| ROTI®Cell Medium 199 / Earle’s | 9112.1 | 1 | 0.7 | X | X | 26.2 | yes | Earle’s | |
| ROTI®Cell TC100 | 9114.2 | 1 | 4,1 | X | X | 4,2 | X | X | With Tryptose Broth. |
| * NEAA: Non-essential amino acids | |||||||||
| Medium | Art. No. | CO2 (%) | Serum | Field of application | Successfully cultured cells |
| Dulbecco’s Modified Eagle Media | |||||
| ROTI®Cell DMEM | 9005.1 | 8,5 | recommend | Frequently used universal medium with up to 4-fold higher concentrations of amino acids and vitamines. | Primary fibroblasts, neurons, glial cells, smooth muscle cells, epithelial cells, tumor cells, hybridome culturesHUVEC, HeLa, HEK 293, PC-12NIH 3T3, 3T6, A-431, A9 L, BALB 3T3, BSC40, COS-1, COS-7, EB, L6, PK1 |
| 9006.1 | |||||
| 9007.1 | |||||
| 1TE9.1 | |||||
| 9010.1 | |||||
| 9019.1 | |||||
| 1TE8.1 | |||||
| 1TE7.1 | |||||
| 9027.1 | |||||
| DMEM : F12 | |||||
| ROTI®Cell DMEM : F12 | 1TE5.1 | 5,5 | optional | Highly complex medium for versatile use.Basis of many serum-free media. | Primary fibroblasts, neurons, HU-VECs, smooth muscle cellsHEK-293, HeLa, Cos-7, PC-12 |
| 1YTP.1 | |||||
| Iscove’s Modfied Dulbecco Media | |||||
| ROTI®Cell Iscove’s MDM | 9033.1 | 7,0 | optional | For fast proliferating cells, high cell densities and mass cell cultures. | Bone marrow cells, lymphocytes, macrophages, Hybridoma culturesKG-1, COS-7, Jurkat |
| Minimum Essential Media | |||||
| ROTI®Cell Eagle’s MEM / Earle’s | 9044.1 | 5,0 | recommend | For cells with special nutritionalrequirements. | Numerous suspension and adherent cells, mammalian fibroblastsHek293, BS-C-1, CV-1, Hep G2, JEG-3, L-929, McCoy, MDBK, MDCK, PK(15), PtK1, RK13, Vero |
| ROTI®Cell Eagle’s MEM / Earle’s | 1TE6.1 | 5,0 | recommend | ||
| ROTI®Cell Eagle’s MEM / Earle’s | 9047.1 | 5,0 | |||
| ROTI®Cell Eagle’s MEM-Alpha | 9058.1 | 5,0 | Additionally suitable for selection of transfected DG44 and other AHFR-negative cells. | BHK-21, BT, Caco-2, CRFK, D-17, HeLa, HT-1080, XC | |
| ROTI®Cell Eagle’s MEM-Alpha | 1TEA.1 | 5,0 | recommend | For cells with special nutritionalrequirements. | |
| RPMI Media | |||||
| ROTI®Cell RPMI 1640 | 9085.1 | 4,5 | recommend | For single cell lines also suitable without serum. | Lymphocytes, leukemia cells, hybridoma cultures, astrocytes, carcinoma cellsDaudi, H9, HCT-15, HL-60, IM-9, Jurkat, K-562, Raji, MCF-7, HeLa, PC12, PBMC |
| 9086.1 | |||||
| 9091.1 | |||||
| 9099.1 | |||||
| 9104.1 | |||||
| Special Media | |||||
| ROTI®Cell Ham’s F12 | 9108.1 | 2,5 | optional | Widely used, highly complex medium. | Specifically for mouse CHO cells, lung cells and L cellsCHO-K1 |
| ROTI®Cell Leibovitz’s L15 | 9109.1 | 0,0* | recommend | Suitable for fast growing cells in relatively high density. | Tumor and embryonic cellsHeLa, Hep-2, SW-13 |
| ROTI®Cell McCoy’s 5A | 9111.1 | 5,0 | recommend | Standard medium for clonal growth of hepatoma cell linesUsed in virus production. | Hepatoma cell lines, other permanent cell lines and also primary cellsHT-29, Jensen |
| ROTI®Cell Medium 199 / Earle’s | 9112.1 | 5,0 | optional | Highly complex medium versatile. | Especially suitable for non-transformed cellsLLC-PK1, LLC-WRC 256 |
| ROTI®Cell TC100 | 9114.2 | 0,0* | recommend | Insect medium for the culture of a variety of Lepidopter species cells. | Sf9, Sf21 |
| * Cultivation outside the CO2 incubator. | |||||
Certificates of Analysis
Type analysis
| pH value | 6.5-7.4 |
| Osmolality | 270-350 mOsm/kg |
| Endotoxins | ≤1 EU/ml |
| Sterility | complies |