Technical Data Sheet
ROTI®Cell RPMI-1640
Sterile products
Ready-to-use products and reagents
Boiling point (bp) ~100 °C
Storage temp. +2 to +8 °C
Transport temp. ambient temp.
WGK 1
€19.25/Pack Qty.
excl. VAT. | 500 ml per Pack Qty.
Art. No. 9104.1
Fits to / Accessories
Product details
RPMI-1640 was developed for the cultivation of normal and neoplastic human leukocytes and is based on modified amounts of amino acids and vitamins. RPMI-1640 Medium is very well suited for culturing of a wide range of mammalian suspension cell lines and primary lymphocytes, all leucocytes, B- and T-lymphocytes, as well as myeloma- and hybridoma cells.
Generally, FBS is added to RPMIl media. However, it has been shown that RPMI media also support several cell lines in the absence of serum.
ROTI®Cell RPMI-1640 CELLPURE® ready-to-use, sterile, w/o glutamine, w/o pyruvate, w/o phenol red
This modification of RPMI does not contain glutamine, pyruvate and phenol red.
| Use | Cell culture |
| Instructions for use, addition | Store at +2 °C to +8 °C protected from light. Once opened, use within 6-8 weeks. |
| Additional info | The detailed composition can be found in the specification. |
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| Art. No. | Pack Qty. | Pack. | Price | Quantity | |
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| 9104.1 | 500 ml | plastic |
€19.25 |
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Available
In procurement
No longer available
Delivery date currently unknown
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Downloads / MSDS
General information
In addition to the various nutrients in the medium, the pH value also plays a very important role in cell culture. The physiological pH for almost all mammalian cells is 7.4. Deviating from this, it has been observed that certain transformed cell lines prefer a pH between 7.0 and 7.4 and certain fibroblasts prefer a pH between 7.4 and 7.7. In any case, pH fluctuations should be avoided and the pH should be maintained as well as possible. That is why a buffer system in media is necessary. The most important buffer system in the body is the carbonic acid-carbonate buffer system. Accordingly, almost all cell culture media contain bicarbonate (NaHCO3). Bicarbonate alone leads to a strong pH increase of the medium, so that NaHCO3-buffered media must always be used with CO2 incubators. Depending on the NaHCO3 concentration in the medium, different CO2 environments are necessary to ensure a pH of about 7.4. For this reason, you should not work outside of the CO2 incubator for too long. If this is necessary, a HEPES buffer should be used in addition to the NaHCO3, which contributes as a second buffer system to stabilize the pH value.
| Medium | Art. No. | Glucose g/l |
Glutamine (mM) |
HEPES (mM) |
Sodium Pyruvate (mM) |
NaHCO3 (mM) |
Phenol Red |
Salts | Note |
| Dulbecco’s Modified Eagle Media | |||||||||
| ROTI®Cell DMEM High Glucose | 9005.1 | 4.5 | 4 | X | 1 | 44 | yes | X | |
| ROTI®Cell DMEM High Glucose | 9006.1 | 4.5 | 4 | X | X | 44 | yes | X | |
| ROTI®Cell DMEM High Glucose | 9007.1 | 4.5 | 4 stab. | X | 1 | 44 | yes | X | With temperature stabile glutamine. |
| ROTI®Cell DMEM High Glucose | 1TE9.1 | 4,5 | 4 stab. | 25 | X | 44 | yes | X | With temperature stabile glutamine. |
| ROTI®Cell DMEM High Glucose | 9010.1 | 4.5 | X | X | 1 | 44 | yes | X | |
| ROTI®Cell DMEM High Glucose | 9019.1 | 4.5 | X | X | X | 44 | yes | X | |
| ROTI®Cell DMEM High Glucose | 1TE8.1 | 4.5 | X | X | X | 44 | X | X | Without phenol red. |
| ROTI®Cell DMEM Low Glucose | 1TE7.1 | 1.0 | 4 stab. | X | 1 | 44 | yes | With temperature stabile glutamine. | |
| ROTI®Cell DMEM Low Glucose | 9027.1 | 1.0 | X | X | 1 | 44 | yes | X | |
| DMEM : F12 | |||||||||
| ROTI®Cell DMEM : F12 | 1TE5.1 | 3.151 | X | 15 | 0,5 | 14.3 | yes | X | |
| ROTI®Cell DMEM : F12 | 1YTP.1 | 3.151 | 2.5 | 15 | 0,5 | 14.3 | yes | X | |
| Iscove’s Modfied Dulbecco Media | |||||||||
| ROTI®Cell Iscove’s MDM | 9033.1 | 4.5 | 4 | 25 | 1 | 36 | yes | X | Additional amino acids and selene. |
| Minimum Essential Media | |||||||||
| ROTI®Cell Eagle’s MEM / Earle’s | 9044.1 | 1 | 2 | X | X | 26.2 | yes | Earle’s | |
| ROTI®Cell Eagle’s MEM / Earle’s | 1TE6.1 | 1 | 2 stab. | X | X | 26.2 | yes | Earle’s | With temperature stabile glutamine. |
| ROTI®Cell Eagle’s MEM / Earle’s | 9047.1 | 1 | X | X | X | 26.2 | yes | Earle’s | |
| ROTI®Cell Eagle’s MEM-Alpha | 9058.1 | 1 | X | X | 1 | 26.2 | yes | Earle’s | Plus cobalamin, ascorbic acid, NEAA*, lipoic acid, biotin. |
| ROTI®Cell Eagle’s MEM-Alpha | 1TEA.1 | 1 | X | X | 1 | 26.2 | yes | Earle’s | Plus cobalamin, ascorbic acid, NEAA*, lipoic acid, biotin, ribonucleosides and desoxyribonucleosides. |
| RPMI Media | |||||||||
| ROTI®Cell RPMI 1640 | 9085.1 | 2 | 2 | X | X | 23.8 | yes | X | |
| ROTI®Cell RPMI 1640 | 9086.1 | 2 | 2 | 25 | X | 23.8 | yes | X | |
| ROTI®Cell RPMI 1640 | 9091.1 | 2 | 2 stab. | X | X | 23.8 | yes | X | With temperature stabile glutamine. |
| ROTI®Cell RPMI 1640 | 9099.1 | 2 | X | X | X | 23.8 | yes | X | |
| ROTI®Cell RPMI 1640 | 9104.1 | 2 | X | X | X | 23.8 | X | X | |
| Special Media | |||||||||
| ROTI®Cell Ham’s F12 | 9108.1 | 1.8 | 1 | X | 1 | 14 | yes | X | |
| ROTI®Cell Leibovitz’s L15 | 9109.1 | 0.9 galact. | X | X | 5 | X | yes | X | With galactose instead of glucose. Without sodium bicarbonate. |
| ROTI®Cell McCoy’s 5A | 9111.1 | 3 | 1.5 | X | X | 26.2 | yes | X | |
| ROTI®Cell Medium 199 / Earle’s | 9112.1 | 1 | 0.7 | X | X | 26.2 | yes | Earle’s | |
| ROTI®Cell TC100 | 9114.2 | 1 | 4,1 | X | X | 4,2 | X | X | With Tryptose Broth. |
| * NEAA: Non-essential amino acids | |||||||||
| Medium | Art. No. | CO2 (%) | Serum | Field of application | Successfully cultured cells |
| Dulbecco’s Modified Eagle Media | |||||
| ROTI®Cell DMEM | 9005.1 | 8,5 | recommend | Frequently used universal medium with up to 4-fold higher concentrations of amino acids and vitamines. | Primary fibroblasts, neurons, glial cells, smooth muscle cells, epithelial cells, tumor cells, hybridome culturesHUVEC, HeLa, HEK 293, PC-12NIH 3T3, 3T6, A-431, A9 L, BALB 3T3, BSC40, COS-1, COS-7, EB, L6, PK1 |
| 9006.1 | |||||
| 9007.1 | |||||
| 1TE9.1 | |||||
| 9010.1 | |||||
| 9019.1 | |||||
| 1TE8.1 | |||||
| 1TE7.1 | |||||
| 9027.1 | |||||
| DMEM : F12 | |||||
| ROTI®Cell DMEM : F12 | 1TE5.1 | 5,5 | optional | Highly complex medium for versatile use.Basis of many serum-free media. | Primary fibroblasts, neurons, HU-VECs, smooth muscle cellsHEK-293, HeLa, Cos-7, PC-12 |
| 1YTP.1 | |||||
| Iscove’s Modfied Dulbecco Media | |||||
| ROTI®Cell Iscove’s MDM | 9033.1 | 7,0 | optional | For fast proliferating cells, high cell densities and mass cell cultures. | Bone marrow cells, lymphocytes, macrophages, Hybridoma culturesKG-1, COS-7, Jurkat |
| Minimum Essential Media | |||||
| ROTI®Cell Eagle’s MEM / Earle’s | 9044.1 | 5,0 | recommend | For cells with special nutritionalrequirements. | Numerous suspension and adherent cells, mammalian fibroblastsHek293, BS-C-1, CV-1, Hep G2, JEG-3, L-929, McCoy, MDBK, MDCK, PK(15), PtK1, RK13, Vero |
| ROTI®Cell Eagle’s MEM / Earle’s | 1TE6.1 | 5,0 | recommend | ||
| ROTI®Cell Eagle’s MEM / Earle’s | 9047.1 | 5,0 | |||
| ROTI®Cell Eagle’s MEM-Alpha | 9058.1 | 5,0 | Additionally suitable for selection of transfected DG44 and other AHFR-negative cells. | BHK-21, BT, Caco-2, CRFK, D-17, HeLa, HT-1080, XC | |
| ROTI®Cell Eagle’s MEM-Alpha | 1TEA.1 | 5,0 | recommend | For cells with special nutritionalrequirements. | |
| RPMI Media | |||||
| ROTI®Cell RPMI 1640 | 9085.1 | 4,5 | recommend | For single cell lines also suitable without serum. | Lymphocytes, leukemia cells, hybridoma cultures, astrocytes, carcinoma cellsDaudi, H9, HCT-15, HL-60, IM-9, Jurkat, K-562, Raji, MCF-7, HeLa, PC12, PBMC |
| 9086.1 | |||||
| 9091.1 | |||||
| 9099.1 | |||||
| 9104.1 | |||||
| Special Media | |||||
| ROTI®Cell Ham’s F12 | 9108.1 | 2,5 | optional | Widely used, highly complex medium. | Specifically for mouse CHO cells, lung cells and L cellsCHO-K1 |
| ROTI®Cell Leibovitz’s L15 | 9109.1 | 0,0* | recommend | Suitable for fast growing cells in relatively high density. | Tumor and embryonic cellsHeLa, Hep-2, SW-13 |
| ROTI®Cell McCoy’s 5A | 9111.1 | 5,0 | recommend | Standard medium for clonal growth of hepatoma cell linesUsed in virus production. | Hepatoma cell lines, other permanent cell lines and also primary cellsHT-29, Jensen |
| ROTI®Cell Medium 199 / Earle’s | 9112.1 | 5,0 | optional | Highly complex medium versatile. | Especially suitable for non-transformed cellsLLC-PK1, LLC-WRC 256 |
| ROTI®Cell TC100 | 9114.2 | 0,0* | recommend | Insect medium for the culture of a variety of Lepidopter species cells. | Sf9, Sf21 |
| * Cultivation outside the CO2 incubator. | |||||
Certificates of Analysis
Type analysis
| pH value | 7.0-7.6 |
| Osmolality | 265-295 mOsm/kg |
| Endotoxins | ≤1,0 EU/ml |
| Sterility | complies |