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Transfer membrane ROTI®NC 0.45 400 × 30 cm

From  Carl ROTH
Examples of effect: Flammable; liquids form compounds with the air which can become explosive; produce flammable gases with water or can self-combust. Safety: Keep away from open flames and sources of heat; close containers tightly; store in a fire-safe manner.
Danger
H228
i flammable solid
P210
i keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking
Material: nitrocellulose (100 %).
Dimensions
ADR 4.1 II
WGK 1
UN-Nr. 3270


€492.35/Pack Qty. 

excl. VAT. | 1 roll(s) per Pack Qty.

Art. No. 9201.1

In production
from 6 Pack Qty. €467.73/Pack Qty.
from 24 Pack Qty. €443.12/Pack Qty.
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Product details

Material: nitrocellulose (100 %).


Transfer membrane ROTI®NC 0.45 400 × 30 cm 

Technical Information
Dimensions 400 × 30 cm 
Version roll 
Pore size 0,45 µm
Bond hydrophobic 
Binding capacity ≥125 µg/cm²
Thickn. 150±10 µm
Immobilisation UV, baking (max. 80 °C) 
Transfer membrane ROTI®NC 0.45 400 × 30 cm
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Art. No. Dimensions Pack Qty. Price Quantity
9201.1 400 × 30 cm 1 roll(s)

€492.35

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Downloads / MSDS

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General information

Technical Parameters of ROTH-Transfer Membranes

Membrane Material Characteristics Thickness (μm) Binding capacity (μg/cm2) Pore size (μm) Immobilisation
Roti® Fluoro PVDF Polyvinyliden fluoride hydrophobic 125 ±25 300 0,2 not necessary
Roti® PVDF 0.2 Polyvinyliden fluoride hydrophobic 140 ±20 ≥125 0,2 not necessary
Roti® PVDF Polyvinyliden fluoride hydrophobic 125 ±25 ca. 200 0,45 not necessary
Immobilon P Polyvinyliden fluoride hydrophobic 120 ±25 150-300 0,45 not necessary
Roti® Nylon 0.2 Nylon 6,6 neutral/amphoteric 150 ±20 500 0,2/0,45 UV, baking
Roti® Nylon plus Nylon 6,6 hydrophilic, positively charged 160 ±20 500 0,45 not necessary, UV and baking possible
Roti® NC Nitrocellulose electrostatic, hydrophobic 150 ±50 125-200 0,2 UV, baking (max. 80 °C)
Roti® NC 0.45 Nitrocellulose electrostatic, hydrophobic 150 ±10 ≥125 0,45 UV, baking (max. 80 °C)
Amersham Protran® Nitrocellulose electrostatic, hydrophobic unknown 11-72 0,2/0,45 UV, baking (max. 80 °C)
Amersham Protran® supported Nitrocellulose electrostatic, hydrophobic unknown 86-110 0,2/0,45 UV, baking (max. 80 °C)

Size Comparison of Membrane Pores and Biomolecules

Biomolecules / Membrane Pores Size (kD)
genomic DNA (human) >11000000
E. coli approx. 1500000
0.45 μm approx. 5000
0.2 μm approx. 2000
DNA 3 kb approx. 1000
RNA 1 kb approx. 300
Antibodies (IgG) approx. 150
Proteins in the lab approx. 10–300
BSA (Albumin) 69

Application of ROTH-Transfer Membranes

Biomolecules Nylon plus Nylon 0.2 PVDF 0.2 PVDF Fluoro PVDF NC NC 0.45
DNA +++ + - - - + +
RNA + +++ - - - + +
Proteins - + +++ +++ +++ + +
Detection System
Colour +++ +++ +++ +++ +++ +++ +++
Radioactivity +++ +++ +++ +++ +++ +++ +++
Chemoluminescence +++ +++ +++ +++ - + +
Fluorescence +++ +++ - - +++ - -
+++: recommended +: suitable -: not