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Transfer membrane ROTI®NC 0.45 400 × 30 cm

From  Carl ROTH
Examples of effect: Flammable; liquids form compounds with the air which can become explosive; produce flammable gases with water or can self-combust. Safety: Keep away from open flames and sources of heat; close containers tightly; store in a fire-safe manner.
Danger
H228
i flammable solid
P210
i keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking
Material: nitrocellulose (100 %).
Dimensions
ADR 4.1 II
WGK 1
UN-Nr. 3270


Transfer membrane for protein analyses, Southern- and Northern-Blots.

High-quality transfer membrane for all detection systems - extremely suitable for all protein transfer and detection methods. The ROTI®NC 0.45 membrane offers very good mechanical stability, standing out for its particularly smooth structure and even membrane thickness. The pure 100 % nitrocellulose quality membrane is easy to block and allows for background-free blots with perfect bands.
Product details

€492.35/Pack Qty. 

excl. VAT. | 1 roll(s) per Pack Qty.

Art. No. 9201.1

In production
from 6 Pack Qty. €467.73/Pack Qty.
from 24 Pack Qty. €443.12/Pack Qty.
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Product details

Material: nitrocellulose (100 %).

Transfer membrane for protein analyses, Southern- and Northern-Blots.

High-quality transfer membrane for all detection systems - extremely suitable for all protein transfer and detection methods. The ROTI®NC 0.45 membrane offers very good mechanical stability, standing out for its particularly smooth structure and even membrane thickness. The pure 100 % nitrocellulose quality membrane is easy to block and allows for background-free blots with perfect bands.


Directions for use

The pore size of 0,45 μm is suitable for proteins and peptides of all sizes. The best retention rate is achieved when blotting proteins of ≥20 kDa. Having a high protein binding capacity, the membrane not only efficiently prevents protein loss during transfer, but also guarantees very sensitive detection of even low protein amounts.

A slight optical difference between the two membrane sides results from the membrane production and has no influence on the binding capacity or the blot quality of the two sides. Both sides can be used equally for transfer and detection.We recommend to always use the optically same side as 'detection side' to achieve maximum reproducibility.


Application examples

Optimised for proteins ≥20 kDa. Western- and far-Western-blots, Southern- and Northern blots, colony- and plaque-lifting of nucleic acids and proteins, dot- and slot blots.
Detection via radio activity, chemoluminescence, colour.



Transfer membrane ROTI®NC 0.45 400 × 30 cm 

Technical Information
Dimensions 400 × 30 cm 
Version roll 
Pore size 0,45 µm
Bond hydrophobic 
Binding capacity ≥125 µg/cm²
Thickn. 150±10 µm
Immobilisation UV, baking (max. 80 °C) 
Transfer membrane ROTI®NC 0.45 400 × 30 cm
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Order No. Dimensions Pack Qty. Price Quantity
9201.1 400 × 30 cm 1 roll(s)

€492.35

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Downloads / MSDS

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General information

Technical Parameters of ROTH-Transfer Membranes

Membrane Material Characteristics Thickness (μm) Binding capacity (μg/cm2) Pore size (μm) Immobilisation
Roti® Fluoro PVDF Polyvinyliden fluoride hydrophobic 125 ±25 300 0,2 not necessary
Roti® PVDF 0.2 Polyvinyliden fluoride hydrophobic 140 ±20 ≥125 0,2 not necessary
Roti® PVDF Polyvinyliden fluoride hydrophobic 125 ±25 ca. 200 0,45 not necessary
Immobilon P Polyvinyliden fluoride hydrophobic 120 ±25 150-300 0,45 not necessary
Roti® Nylon 0.2 Nylon 6,6 neutral/amphoteric 150 ±20 500 0,2/0,45 UV, baking
Roti® Nylon plus Nylon 6,6 hydrophilic, positively charged 160 ±20 500 0,45 not necessary, UV and baking possible
Roti® NC Nitrocellulose electrostatic, hydrophobic 150 ±50 125-200 0,2 UV, baking (max. 80 °C)
Roti® NC 0.45 Nitrocellulose electrostatic, hydrophobic 150 ±10 ≥125 0,45 UV, baking (max. 80 °C)
Amersham Protran® Nitrocellulose electrostatic, hydrophobic unknown 11-72 0,2/0,45 UV, baking (max. 80 °C)
Amersham Protran® supported Nitrocellulose electrostatic, hydrophobic unknown 86-110 0,2/0,45 UV, baking (max. 80 °C)

Size Comparison of Membrane Pores and Biomolecules

Biomolecules / Membrane Pores Size (kD)
genomic DNA (human) >11000000
E. coli approx. 1500000
0.45 μm approx. 5000
0.2 μm approx. 2000
DNA 3 kb approx. 1000
RNA 1 kb approx. 300
Antibodies (IgG) approx. 150
Proteins in the lab approx. 10–300
BSA (Albumin) 69

Application of ROTH-Transfer Membranes

Biomolecules Nylon plus Nylon 0.2 PVDF 0.2 PVDF Fluoro PVDF NC NC 0.45
DNA +++ + - - - + +
RNA + +++ - - - + +
Proteins - + +++ +++ +++ + +
Detection System
Colour +++ +++ +++ +++ +++ +++ +++
Radioactivity +++ +++ +++ +++ +++ +++ +++
Chemoluminescence +++ +++ +++ +++ - + +
Fluorescence +++ +++ - - +++ - -
+++: recommended +: suitable -: not