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Transfer membrane ROTI®Nylon plus, 15 × 10 cm

From  Carl ROTH
Material: nylon 6.6.
Dimensions


€161.70/Pack Qty. 

excl. VAT. | 10 unit(s) per Pack Qty.

Art. No. HP35.1

In stock
Delivery fast, simple and reliable!
from 6 Pack Qty. €153.62/Pack Qty.
from 24 Pack Qty. €145.53/Pack Qty.

Product details

Material: nylon 6.6.

.




Transfer membrane ROTI®Nylon plus 

Ideal for DNA/RNA-transfers.

ROTI®Nylon plus is a cationic, einforced nylon 6.6-membrane with extremely high tensile strength. The positively charged membrane surface guarantees rapid and efficient binding of DNA and RNA. May be steam sterilised.
Also available in convenient pre-cut sheets of standard gel size!

Nucleic acids can be cross-linked to the membrane by baking (80 °C, 15 min) or through UV exposure. No cross-linking is required for alkaline transfer as the negatively charged DNA forms a very stable complex with the positively charged ROTI®Nylon membrane.
Detection systems: radioactivity, chemoluminescence, fluorescence

Application examples:
  • Southern and Northern blot
  • Dot-Blot
  • DNA fngerprinting
  • Macro- and Microarrays


Technical Information
Dimensions 15 × 10 cm 
Version sheet 
Pore size 0.45 µm
Type 0.45 µm 
Bond hydrophilique 
Load positive 
Binding capacity 500 µg/cm²
Thickn. 160±20 µm
Immobilisation not necessary, UV and baking possible 
Transfer membrane ROTI®Nylon plus
Selected quantity:   0
  1. Subtotal:  0.00
Art. No. Dimensions Pack Qty. Price Quantity
HP35.1 15 × 10 cm 10 unit(s)

€161.70

HP36.1 20 × 20 cm 10 unit(s)

€235.45

K058.1 300 × 30 cm 1 roll(s)

€339.00

In stock
Available
In procurement
No longer available
Delivery date currently unknown
Selected quantity:   0
  1. Subtotal:  0.00

Downloads / MSDS

General information

Technical Parameters of ROTH-Transfer Membranes

Membrane Material Characteristics Thickness (μm) Binding capacity (μg/cm2) Pore size (μm) Immobilisation
Roti® Fluoro PVDF Polyvinyliden fluoride hydrophobic 125 ±25 300 0,2 not necessary
Transfer membrane ROTI®PVDF 0.2 Polyvinyliden fluoride hydrophobic 190 ±50 ≥125 0,2 not necessary
Transfer membrane ROTI®PVDF 0.45 Polyvinyliden fluoride hydrophobic 190 ±50 ≥125 0,45 not necessary
Immobilon P Polyvinyliden fluoride hydrophobic 120 ±25 150-300 0,45 not necessary
Roti® Nylon 0.2 Nylon 6,6 neutral/amphoteric 150 ±20 500 0,2/0,45 UV, baking
Roti® Nylon plus Nylon 6,6 hydrophilic, positively charged 160 ±20 500 0,45 not necessary, UV and baking possible
Transfer membrane ROTI®NC 0.2 Nitrocellulose hydrophobic 150 ±50 100 0,2 UV, baking (max. 80 °C)
Transfer membrane ROTI®NC 0.45 Nitrocellulose hydrophobic 150 ±50 100 0,45 UV, baking (max. 80 °C)
Amersham Protran® Nitrocellulose electrostatic, hydrophobic unknown 11-72 0,2/0,45 UV, baking (max. 80 °C)
Amersham Protran® supported Nitrocellulose electrostatic, hydrophobic unknown 86-110 0,2/0,45 UV, baking (max. 80 °C)

Size Comparison of Membrane Pores and Biomolecules

Biomolecules / Membrane Pores Size (kD)
genomic DNA (human) >11000000
E. coli approx. 1500000
0.45 μm approx. 5000
0.2 μm approx. 2000
DNA 3 kb approx. 1000
RNA 1 kb approx. 300
Antibodies (IgG) approx. 150
Proteins in the lab approx. 10–300
BSA (Albumin) 69

Application of ROTH-Transfer Membranes

Biomolecules Nylon plus Nylon 0.2 PVDF 0.2 PVDF Fluoro PVDF NC NC 0.45
DNA +++ + - - - + +
RNA + +++ - - - + +
Proteins - + +++ +++ +++ + +
Detection System
Colour +++ +++ +++ +++ +++ +++ +++
Radioactivity +++ +++ +++ +++ +++ +++ +++
Chemoluminescence +++ +++ +++ +++ - + +
Fluorescence +++ +++ - - +++ - -
+++: recommended +: suitable -: not

Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found: