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Transfer membrane ROTI®Nylon plus, 300 × 30 cm

From  Carl ROTH
Material: nylon 6.6.
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We no longer stock this product in our range. See alternative products.


€339.00/Pack Qty. 

excl. VAT. | 1 roll(s) per Pack Qty.

Art. No. K058.1

No longer available
from 6 Pack Qty. €322.05/Pack Qty.
from 24 Pack Qty. €305.10/Pack Qty.

Product details

Material: nylon 6.6.

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Transfer membrane ROTI®Nylon plus 

Ideal for DNA/RNA-transfers.

ROTI®Nylon plus is a cationic, einforced nylon 6.6-membrane with extremely high tensile strength. The positively charged membrane surface guarantees rapid and efficient binding of DNA and RNA. May be steam sterilised.
Also available in convenient pre-cut sheets of standard gel size!

Nucleic acids can be cross-linked to the membrane by baking (80 °C, 15 min) or through UV exposure. No cross-linking is required for alkaline transfer as the negatively charged DNA forms a very stable complex with the positively charged ROTI®Nylon membrane.
Detection systems: radioactivity, chemoluminescence, fluorescence

Application examples:
  • Southern and Northern blot
  • Dot-Blot
  • DNA fngerprinting
  • Macro- and Microarrays


Technical Information
Dimensions 300 × 30 cm 
Version roll 
Pore size 0.45 µm
Type 0.45 µm 
Bond hydrophilique 
Load positive 
Binding capacity 500 µg/cm²
Thickn. 160±20 µm
Immobilisation not necessary, UV and baking possible 
Transfer membrane ROTI®Nylon plus
Selected quantity:   0
  1. Subtotal:  0.00
Art. No. Dimensions Pack Qty. Price Quantity
K058.1 300 × 30 cm 1 roll(s)

€339.00

HP36.1 20 × 20 cm 10 unit(s)

€235.45

HP35.1 15 × 10 cm 10 unit(s)

€161.70

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Available
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Selected quantity:   0
  1. Subtotal:  0.00

Downloads / MSDS

General information

Technical Parameters of ROTH-Transfer Membranes

Membrane Material Characteristics Thickness (μm) Binding capacity (μg/cm2) Pore size (μm) Immobilisation
Roti® Fluoro PVDF Polyvinyliden fluoride hydrophobic 125 ±25 300 0,2 not necessary
Transfer membrane ROTI®PVDF 0.2 Polyvinyliden fluoride hydrophobic 190 ±50 ≥125 0,2 not necessary
Transfer membrane ROTI®PVDF 0.45 Polyvinyliden fluoride hydrophobic 190 ±50 ≥125 0,45 not necessary
Immobilon P Polyvinyliden fluoride hydrophobic 120 ±25 150-300 0,45 not necessary
Roti® Nylon 0.2 Nylon 6,6 neutral/amphoteric 150 ±20 500 0,2/0,45 UV, baking
Roti® Nylon plus Nylon 6,6 hydrophilic, positively charged 160 ±20 500 0,45 not necessary, UV and baking possible
Transfer membrane ROTI®NC 0.2 Nitrocellulose hydrophobic 150 ±50 100 0,2 UV, baking (max. 80 °C)
Transfer membrane ROTI®NC 0.45 Nitrocellulose hydrophobic 150 ±50 100 0,45 UV, baking (max. 80 °C)
Amersham Protran® Nitrocellulose electrostatic, hydrophobic unknown 11-72 0,2/0,45 UV, baking (max. 80 °C)
Amersham Protran® supported Nitrocellulose electrostatic, hydrophobic unknown 86-110 0,2/0,45 UV, baking (max. 80 °C)

Size Comparison of Membrane Pores and Biomolecules

Biomolecules / Membrane Pores Size (kD)
genomic DNA (human) >11000000
E. coli approx. 1500000
0.45 μm approx. 5000
0.2 μm approx. 2000
DNA 3 kb approx. 1000
RNA 1 kb approx. 300
Antibodies (IgG) approx. 150
Proteins in the lab approx. 10–300
BSA (Albumin) 69

Application of ROTH-Transfer Membranes

Biomolecules Nylon plus Nylon 0.2 PVDF 0.2 PVDF Fluoro PVDF NC NC 0.45
DNA +++ + - - - + +
RNA + +++ - - - + +
Proteins - + +++ +++ +++ + +
Detection System
Colour +++ +++ +++ +++ +++ +++ +++
Radioactivity +++ +++ +++ +++ +++ +++ +++
Chemoluminescence +++ +++ +++ +++ - + +
Fluorescence +++ +++ - - +++ - -
+++: recommended +: suitable -: not

Certificates of Analysis

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