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Technical Data Sheet
T831 Membran_Demo

Transfer membraneImmobilon®-P
From  MERCK MILLIPORE
Material: polyvinylidene fluoride (PVDF).
Dimensions


Original PVDF membrane from Millipore, for outstanding Western Blots.

The Immobilon-P membrane consists of microporous polyvinylidene fluoride (PVDF) with a high binding capacity for all biomolecules, particularly for proteins, independently of the transfer method or gel used. The efficient signal-to-noise ratio allows long exposure times and provides a high sensitivity during detection even when working with low protein amounts.
Product details

€562.25/Pack Qty. 

excl. VAT. | 1 roll(s) per Pack Qty.

Art. No. T831.1

In stock
Delivery fast, simple and reliable!
from 6 Pack Qty. €534.14/Pack Qty.
from 24 Pack Qty. €506.02/Pack Qty.

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Product details

Material: polyvinylidene fluoride (PVDF).

Original PVDF membrane from Millipore, for outstanding Western Blots.

The Immobilon-P membrane consists of microporous polyvinylidene fluoride (PVDF) with a high binding capacity for all biomolecules, particularly for proteins, independently of the transfer method or gel used. The efficient signal-to-noise ratio allows long exposure times and provides a high sensitivity during detection even when working with low protein amounts.


Directions for use

Compared to nitrocellulose the Immobilon-P membrane is much easier to handle and much better to stain, and shows superior resistance to organic solvents. Furthermore, the open pore structure provides easy access of antibodies or other peptides used for detection to bound protein, and enables efficient removal of unbound molecules, efficiently reducing the background.
Immobilon-PVDF membrane is ideal when performing immunodetection. The rapid immunodetection method additionally provides a tool to reduce the time span needed for the process by several hours. During Western Blot, the superior qualities of the PVDF membrane not only supersede a blocking step, but also reduce number and time span of washing steps considerably without compromising on specificity or sensitivity.


Application examples

Western-blot, Dot- and Slot-blot, amino acid analysis, binding assays, detection of glycoproteins, N-terminal peptide sequencing.
Detection systems: radioactivity, chemoluminescence, colour



Transfer membraneImmobilon®-P 

Technical Information
Dimensions 375 × 28 cm 
Version roll 
Pore size 0,45 µm
Bond hydrophobic 
Binding capacity 150–300 µg/cm²
Thickn. 125±25 µm
Immobilisation not necessary 
Transfer membraneImmobilon®-P
Selected quantity:   0
  1. Subtotal:  0.00
Art. No. Dimensions Pack Qty. Price Quantity
T831.1 375 × 28 cm 1 roll(s)

€562.25

In stock
Available
In procurement
No longer available
Delivery date currently unknown
Selected quantity:   0
  1. Subtotal:  0.00

Downloads / MSDS

Technical Information / German
Technical Information / English

General information

Technical Parameters of ROTH-Transfer Membranes

Membrane Material Characteristics Thickness (μm) Binding capacity (μg/cm2) Pore size (μm) Immobilisation
Roti® Fluoro PVDF Polyvinyliden fluoride hydrophobic 125 ±25 300 0,2 not necessary
Transfer membrane ROTI®PVDF 0.2 Polyvinyliden fluoride hydrophobic 190 ±50 ≥125 0,2 not necessary
Transfer membrane ROTI®PVDF 0.45 Polyvinyliden fluoride hydrophobic 190 ±50 ≥125 0,45 not necessary
Immobilon P Polyvinyliden fluoride hydrophobic 120 ±25 150-300 0,45 not necessary
Roti® Nylon 0.2 Nylon 6,6 neutral/amphoteric 150 ±20 500 0,2/0,45 UV, baking
Roti® Nylon plus Nylon 6,6 hydrophilic, positively charged 160 ±20 500 0,45 not necessary, UV and baking possible
Transfer membrane ROTI®NC 0.2 Nitrocellulose hydrophobic 150 ±50 100 0,2 UV, baking (max. 80 °C)
Transfer membrane ROTI®NC 0.45 Nitrocellulose hydrophobic 150 ±50 100 0,45 UV, baking (max. 80 °C)
Amersham Protran® Nitrocellulose electrostatic, hydrophobic unknown 11-72 0,2/0,45 UV, baking (max. 80 °C)
Amersham Protran® supported Nitrocellulose electrostatic, hydrophobic unknown 86-110 0,2/0,45 UV, baking (max. 80 °C)

Size Comparison of Membrane Pores and Biomolecules

Biomolecules / Membrane Pores Size (kD)
genomic DNA (human) >11000000
E. coli approx. 1500000
0.45 μm approx. 5000
0.2 μm approx. 2000
DNA 3 kb approx. 1000
RNA 1 kb approx. 300
Antibodies (IgG) approx. 150
Proteins in the lab approx. 10–300
BSA (Albumin) 69

Application of ROTH-Transfer Membranes

Biomolecules Nylon plus Nylon 0.2 PVDF 0.2 PVDF Fluoro PVDF NC NC 0.45
DNA +++ + - - - + +
RNA + +++ - - - + +
Proteins - + +++ +++ +++ + +
Detection System
Colour +++ +++ +++ +++ +++ +++ +++
Radioactivity +++ +++ +++ +++ +++ +++ +++
Chemoluminescence +++ +++ +++ +++ - + +
Fluorescence +++ +++ - - +++ - -
+++: recommended +: suitable -: not

Certificates of Analysis

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