Technical Data Sheet
Transfer membrane ROTI®NC 0.2, Ø 9.0 cm
From Carl ROTH
Material: nitrocellulose (100 %).
Related products
Dimensions
€184.90/Pack Qty.
excl. VAT. | 50 sheet(s) per Pack Qty.
Art. No. HP44.1
No longer available
from 6 Pack Qty.
€175.66/Pack Qty.
from 24 Pack Qty.
€166.41/Pack Qty.
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Product details
Material: nitrocellulose (100 %).
Not a medical device / Not an IVD product
Technical Information
| Dimensions | Ø 9.0 cm |
| Version | disk |
| Pore size | 0,2 µm |
| Bond | hydrophobic |
| Binding capacity | 100 µg/cm² |
| Thickn. | 150±50 µm |
| Immobilisation | UV, baking (max. 80 °C) |
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| Art. No. | Dimensions | Pack Qty. | Price | Quantity | |
|---|---|---|---|---|---|
| HP44.1 | Ø 9.0 cm | 50 sheet(s) |
€184.90 |
|
|
| 1YXN.1 | 300 × 30 cm | 1 roll(s) |
€387.00 |
|
|
| 200E.1 | 300 × 20 cm | 1 roll(s) |
€263.50 |
|
|
| 200H.1 | 20 × 20 cm | 5 sheet(s) |
€129.00 |
|
|
| 204H.1 | 10 × 10 cm | 10 sheet(s) |
€118.30 |
|
|
|
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General information
Technical Parameters of ROTH-Transfer Membranes
| Membrane | Material | Characteristics | Thickness (μm) | Binding capacity (μg/cm2) | Pore size (μm) | Immobilisation |
| Roti® Fluoro PVDF | Polyvinyliden fluoride | hydrophobic | 125 ±25 | 300 | 0,2 | not necessary |
| Roti® PVDF 0.2 | Polyvinyliden fluoride | hydrophobic | 140 ±20 | ≥125 | 0,2 | not necessary |
| Roti® PVDF | Polyvinyliden fluoride | hydrophobic | 125 ±25 | ca. 200 | 0,45 | not necessary |
| Immobilon P | Polyvinyliden fluoride | hydrophobic | 120 ±25 | 150-300 | 0,45 | not necessary |
| Roti® Nylon 0.2 | Nylon 6,6 | neutral/amphoteric | 150 ±20 | 500 | 0,2/0,45 | UV, baking |
| Roti® Nylon plus | Nylon 6,6 | hydrophilic, positively charged | 160 ±20 | 500 | 0,45 | not necessary, UV and baking possible |
| Roti® NC | Nitrocellulose | electrostatic, hydrophobic | 150 ±50 | 125-200 | 0,2 | UV, baking (max. 80 °C) |
| Roti® NC 0.45 | Nitrocellulose | electrostatic, hydrophobic | 150 ±10 | ≥125 | 0,45 | UV, baking (max. 80 °C) |
| Amersham Protran® | Nitrocellulose | electrostatic, hydrophobic | unknown | 11-72 | 0,2/0,45 | UV, baking (max. 80 °C) |
| Amersham Protran® supported | Nitrocellulose | electrostatic, hydrophobic | unknown | 86-110 | 0,2/0,45 | UV, baking (max. 80 °C) |
Size Comparison of Membrane Pores and Biomolecules
| Biomolecules / Membrane Pores | Size (kD) |
| genomic DNA (human) | >11000000 |
| E. coli | approx. 1500000 |
| 0.45 μm | approx. 5000 |
| 0.2 μm | approx. 2000 |
| DNA 3 kb | approx. 1000 |
| RNA 1 kb | approx. 300 |
| Antibodies (IgG) | approx. 150 |
| Proteins in the lab | approx. 10–300 |
| BSA (Albumin) | 69 |
Application of ROTH-Transfer Membranes
| Biomolecules | Nylon plus | Nylon 0.2 | PVDF 0.2 | PVDF | Fluoro PVDF | NC | NC 0.45 |
| DNA | +++ | + | - | - | - | + | + |
| RNA | + | +++ | - | - | - | + | + |
| Proteins | - | + | +++ | +++ | +++ | + | + |
| Detection System | |||||||
| Colour | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
| Radioactivity | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
| Chemoluminescence | +++ | +++ | +++ | +++ | - | + | + |
| Fluorescence | +++ | +++ | - | - | +++ | - | - |
| +++: recommended | +: suitable | -: not | |||||