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Technical Data Sheet
Membran_Demo

Transfer membrane ROTI® ProBind NC 0.2
From  Carl ROTH
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Examples of effect: Flammable; liquids form compounds with the air which can become explosive; produce flammable gases with water or can self-combust. Safety: Keep away from open flames and sources of heat; close containers tightly; store in a fire-safe manner.
Danger
H228
i flammable solid
P210
i keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking
ADR 4.1 II
WGK 1
UN-Nr. 3270


Transfer Membrane for Nucleic Acid and Protein Immobilization Techniques

ProBind transfer membranes are the ideal choice for reliable protein analysis, Southern and Northern blots. Available as PVDF or nitrocellulose (NC) membranes, they offer high mechanical stability, excellent binding capacity, and minimal background interference. Their uniform structure ensures sharp and clear bands for precise results.Thanks to their high chemical resistance and optimal sensitivity, ProBind transfer membranes are perfect for a wide range of detection methods—from classic blotting techniques to advanced assays.
Product details

excl. VAT.

Art. No. 2600409

Product details


Transfer Membrane for Nucleic Acid and Protein Immobilization Techniques

ProBind transfer membranes are the ideal choice for reliable protein analysis, Southern and Northern blots. Available as PVDF or nitrocellulose (NC) membranes, they offer high mechanical stability, excellent binding capacity, and minimal background interference. Their uniform structure ensures sharp and clear bands for precise results.Thanks to their high chemical resistance and optimal sensitivity, ProBind transfer membranes are perfect for a wide range of detection methods—from classic blotting techniques to advanced assays.



Transfer membrane ROTI® ProBind NC 0.2 

Applications:
  • Western Blotting
  • Southern Blotting
  • Northern Blotting
  • Amino Acid and Protein Analysis


Transfer membrane ROTI® ProBind NC 0.2
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Security datasheet
Switzerland / English
Security datasheet
Germany / English

General information

Technical Parameters of ROTH-Transfer Membranes

Membrane Material Characteristics Thickness (μm) Binding capacity (μg/cm2) Pore size (μm) Immobilisation
Roti® Fluoro PVDF Polyvinyliden fluoride hydrophobic 125 ±25 300 0,2 not necessary
Transfer membrane ROTI®PVDF 0.2 Polyvinyliden fluoride hydrophobic 190 ±50 ≥125 0,2 not necessary
Transfer membrane ROTI®PVDF 0.45 Polyvinyliden fluoride hydrophobic 190 ±50 ≥125 0,45 not necessary
Immobilon P Polyvinyliden fluoride hydrophobic 120 ±25 150-300 0,45 not necessary
Roti® Nylon 0.2 Nylon 6,6 neutral/amphoteric 150 ±20 500 0,2/0,45 UV, baking
Roti® Nylon plus Nylon 6,6 hydrophilic, positively charged 160 ±20 500 0,45 not necessary, UV and baking possible
Transfer membrane ROTI®NC 0.2 Nitrocellulose hydrophobic 150 ±50 100 0,2 UV, baking (max. 80 °C)
Transfer membrane ROTI®NC 0.45 Nitrocellulose hydrophobic 150 ±50 100 0,45 UV, baking (max. 80 °C)
Amersham Protran® Nitrocellulose electrostatic, hydrophobic unknown 11-72 0,2/0,45 UV, baking (max. 80 °C)
Amersham Protran® supported Nitrocellulose electrostatic, hydrophobic unknown 86-110 0,2/0,45 UV, baking (max. 80 °C)

Size Comparison of Membrane Pores and Biomolecules

Biomolecules / Membrane Pores Size (kD)
genomic DNA (human) >11000000
E. coli approx. 1500000
0.45 μm approx. 5000
0.2 μm approx. 2000
DNA 3 kb approx. 1000
RNA 1 kb approx. 300
Antibodies (IgG) approx. 150
Proteins in the lab approx. 10–300
BSA (Albumin) 69

Application of ROTH-Transfer Membranes

Biomolecules Nylon plus Nylon 0.2 PVDF 0.2 PVDF Fluoro PVDF NC NC 0.45
DNA +++ + - - - + +
RNA + +++ - - - + +
Proteins - + +++ +++ +++ + +
Detection System
Colour +++ +++ +++ +++ +++ +++ +++
Radioactivity +++ +++ +++ +++ +++ +++ +++
Chemoluminescence +++ +++ +++ +++ - + +
Fluorescence +++ +++ - - +++ - -
+++: recommended +: suitable -: not