Technical Data Sheet
5-Ethynyl uridine (EU), 10 mg
All products identified as being particularly temperaturesensitive are shipped in special ice boxes with freezer packs or in dry ice.
Molar mass (M) 268,22 g/mol
Storage temp. -20 °C
Transport temp. cooled
WGK 1
CAS No. 69075-42-9
5-Ethynyl uridine (5-EU) is a modified nucleoside that can be used for labeling of nascent RNA inside cells. After feeding EU, it can react with azides. Our own experiments indicate that it will be first integrated in de novo synthesized RNA, but we have also observed incorporation into DNA after some incubation time. This is most likely via conversion of the EU ribonucleoside into EdU aided by intracellular ribonucleotide reductases. Research from several groups demonstrates successful EU cellular labeling for divers applications.
€69.90/Pack Qty.
excl. VAT. | 10 mg per Pack Qty.
Art. No. 7848.2
- Subtotal: 0.00
| Art. No. | Pack Qty. | Pack. | Price | Quantity | |
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| 7848.1 | 5 mg | plastic |
€51.50 |
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| 7848.2 | 10 mg | plastic |
€69.90 |
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| 7848.3 | 100 mg | plastic |
€430.00 |
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Downloads / MSDS
General information
The copper(I)-catalysed azide-alkyne cycloaddition (CuAAC) is the most prominent example of a group of reactions named click reactions. According to Sharpless’ definition, these reactions are characterised by high yields, mild reaction conditions, and by their tolerance of a broad range of functional groups.[1-3] Typically, the reactions require simple or no workup or purification of the product. The most important characteristic of the CuAAC reaction is its unique bioorthogonality as neither azide nor terminal alkyne functional groups are generally present in natural systems.
References:
1 H. C. Kolb, M.G. Finn, K. B. Sharpless, Angew. Chem. Int. Ed. 2001, 40, 2004-2021.
2 C.W. Tornoe, C. Christensen, M. Meldal, J. Org. Chem. 2002, 67, 3057-3064.
3 V. V. Rostovtsev, L. G. Green, V. V. Fokin, K. B. Sharpless, Angew. Chem. 2002, 114, 2708-2711; Angew. Chem. Int. Ed. 2002, 41, 2596-2599.
In recent years, click chemistry has become increasingly important and steadily more popular in research and development. The reasons for this are not only the easy handling and the high yields but also the countless application possibilities. Carl ROTH offers you the possibility to purchase the broad portfolio of click reagents, various kits for EdU-based cell proliferation assays and numerous other applications from the well-known brand baseclick. This enables us to offer you a necessary and comprehensive assortment for click chemistry.
Nucleosides
The detection of cell proliferation is of utmost importance for monitoring cell vitality, determining genotoxicity or evaluating anticancer drugs. The feeding of cells with EdU (5-Ethynyl-2'-deoxyuridine) or EU (5-Ethynyl uridine) can be used in cell proliferation studies.
Certificates of Analysis
Type analysis
| Appearance | white to light brown to light green powder |
| Assay (HPLC) | ≥98 % |