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5-Ethynyl-dUTP, 10 µl

From  baseclick
≥95 %, 100 mM in water
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EdUTP, 5-Ethynyl-2’-deoxyuridine-5’-triphosphate
Empirical formula C11H15N2O14P3
Molar mass (M) 492,16 g/mol
Density (D) ~1 g/cm³
Storage temp. -20 °C
Transport temp. cooled
WGK 1
CAS No. 1004955-96-7

Modified triphosphate for incorporation in PCR reaction
5-Ethynyl-dUTP (5-EdUTP) is easily incorporated into DNA by PCR instead of dUTP using polymerases like Pwo, Deep Vent exo- or KOD XL. The resulting stable and modified PCR fragment is now ready for further functionalization by Click Chemistry. We recommend a substitution of 1 – 10% of dUTP.

€147.85/Pack Qty. 

excl. VAT. | 10 µl per Pack Qty.

Art. No. 7802.1

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7802.1 10 µl plastic

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Downloads / MSDS

General information

Click Chemistry

The copper(I)-catalysed azide-alkyne cycloaddition (CuAAC) is the most prominent example of a group of reactions named click reactions. According to Sharpless’ definition, these reactions are characterised by high yields, mild reaction conditions, and by their tolerance of a broad range of functional groups.[1-3] Typically, the reactions require simple or no workup or purification of the product. The most important characteristic of the CuAAC reaction is its unique bioorthogonality as neither azide nor terminal alkyne functional groups are generally present in natural systems.


References:
1 H. C. Kolb, M.G. Finn, K. B. Sharpless, Angew. Chem. Int. Ed. 2001, 40, 2004-2021.
2 C.W. Tornoe, C. Christensen, M. Meldal, J. Org. Chem. 2002, 67, 3057-3064.
3 V. V. Rostovtsev, L. G. Green, V. V. Fokin, K. B. Sharpless, Angew. Chem. 2002, 114, 2708-2711; Angew. Chem. Int. Ed. 2002, 41, 2596-2599.


Click Chemistry

In recent years, click chemistry has become increasingly important and steadily more popular in research and development. The reasons for this are not only the easy handling and the high yields but also the countless application possibilities. Carl ROTH offers you the possibility to purchase the broad portfolio of click reagents, various kits for EdU-based cell proliferation assays and numerous other applications from the well-known brand baseclick. This enables us to offer you a necessary and comprehensive assortment for click chemistry.


Nucleotides

Our modified nucleotides for click chemistry complement our range of unmodified standard nucleotides. You can use our alkyne-triphosphates in your nucleotide mixture to incorporate alkyne groups into your PCR fragment. These alkyne groups can be used to label the PCR fragment in a subsequent click reaction.


Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Type analysis

Appearanceclear solution
Assay (HPLC)≥95 %