5-Ethynyl-dUTP, 25 µl
Molar mass (M) 491,97 g/mol
Density (D) 1 g/cm³
Boiling point (bp) 100 °C
Melting point (mp) 0 °C
Storage temp. -20 °C
Transport temp. cooled
CAS No. [1004955-96-7]
excl. VAT. | 25 µl per Pack Qty.
Art. No. 7802.2
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The copper(I)-catalysed azide-alkyne cycloaddition (CuAAC) is the most prominent example of a group of reactions named click reactions. According to Sharpless’ definition, these reactions are characterised by high yields, mild reaction conditions, and by their tolerance of a broad range of functional groups.[1-3] Typically, the reactions require simple or no workup or purification of the product. The most important characteristic of the CuAAC reaction is its unique bioorthogonality as neither azide nor terminal alkyne functional groups are generally present in natural systems.
1 H. C. Kolb, M.G. Finn, K. B. Sharpless, Angew. Chem. Int. Ed. 2001, 40, 2004-2021.
2 C.W. Tornoe, C. Christensen, M. Meldal, J. Org. Chem. 2002, 67, 3057-3064.
3 V. V. Rostovtsev, L. G. Green, V. V. Fokin, K. B. Sharpless, Angew. Chem. 2002, 114, 2708-2711; Angew. Chem. Int. Ed. 2002, 41, 2596-2599.
Our modified nucleotides for click chemistry complement our range of unmodified standard nucleotides. You can use our alkyne-triphosphates in your nucleotide mixture to incorporate alkyne groups into your PCR fragment. These alkyne groups can be used to label the PCR fragment in a subsequent click reaction.
|Assay (HPLC)||≥98 %|