Technical Data Sheet
Transfer membrane ROTI®Nylon 0.45, 15 × 15 cm
All autoclavable products in our catalogue are identified by this symbol. Please also note any additional instructions when autoclaving.
€94.60/Pack Qty. Campaign price!
excl. VAT. | 5 sheet(s) per Pack Qty.
Art. No. 260Y.1
Product details
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Transfer membrane ROTI®Nylon 0.45
The optimal membrane for DNA/RNA transfer, especially colony and plaque lifting and all multiple rehybridisations.
ROTI®Nylon 0.45 is a neutral, reinforced nylon membrane. Like all ROTI®Nylon membranes, it has a very high tensile strength and is highly resistant to alcohols, acetone and many other organic solvents.
ROTI®Nylon 0.45 is not charged at buffer pH 6.5 and has excellent hydrophilic properties due to the amino and carboxy groups on the surface. The membrane charge (ζ-potential) can be adjusted by the pH value of the buffer system (positive membrane charge at pH<6.5; negative membrane charge at pH>6.5). Steam sterilisation is possible.
The covalent binding of transferred nucleic acid is achieved by crosslinking (UV crosslinking for 20 s at 260 nm). The excellent signal/background ratio of the membrane then enables highly sensitive detections (single copy gene detection) and multiple rehybridisations without loss of signal quality.
Detection by radioactivity, chemiluminescence, fluorescence
- Southern and Northern blotting
- Western blotting
- Microarrays and Macroarrays
- Dot/Slot blotting
| Dimensions | 15 × 15 cm |
| Pore size | 0,45 µm |
| Bond | hydrophile |
| Load | neutral/amphoteric |
| Binding capacity | 350 µg/cm² |
| Thickn. | 140-190 µm |
| Immobilisation | UV |
- Subtotal: 0.00
| Art. No. | Dimensions | Pack Qty. | Price | Quantity | |
|---|---|---|---|---|---|
| 260P.1 | 300 × 30 cm | 1 roll(s) |
€456.90
€365.50 |
|
|
| 260T.1 | 300 × 15 cm | 1 roll(s) |
€236.50
€189.20 |
|
|
| 260X.1 | 20 × 20 cm | 5 sheet(s) |
€166.65
€133.30 |
|
|
| 260Y.1 | 15 × 15 cm | 5 sheet(s) |
€118.25
€94.60 |
|
|
|
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- Subtotal: 0.00