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ROTI®Sep 1077 human, 100 ml

CELLPURE® sterile, ready-to-use, for density gradient centrifugation
Icon_Steril Sterile products
Icon_ready-to-use Ready-to-use products and reagents
Examples of effect: Have an allergenic effect, carcinogenic, mutagenic, toxic to reproduction and cause developmental toxicity (reprotoxic) or damage to organs Safety: You must be well-informed before starting work with these substances; wear protective clothing and gloves, eye protection and mask or breathing protection.
Danger
H317-H334
i may cause an allergic skin reaction, may cause allergy or asthma symptoms or breathing difficulties if inhaled
P261 P302+P352 P333+P313
i avoid breathing mist/vapours, IF ON SKIN: Wash with plenty of water, if skin irritation or rash occurs: Get medical advice/attention
Sterile
Pack Qty.
Pack.
Lymphocyte separation medium, Polysucrose solution
Molar mass (M) ~400000 g/mol
Density (D) 1,08 g/cm³
Boiling point (bp) 100 °C
Storage temp. +4 °C
WGK 1

Polysucrose based medium for separation of lymphocytes/PBMC from human whole blood. Density: 1,077 g/ml
Long-term storage at approx. 4 °C recommended. Short-term storage at room temperature possible. Storage protected from light.

Ready-to-use and sterile separation medium based on polysucrose 400, for efficient isolation of lymphocytes from human blood by density gradient centrifugation.
Density 1.077 g/ml.
Best alternative to Ficoll®.
Product details

€63.35/Pack Qty. 

excl. VAT. | 100 ml per Pack Qty.

Art. No. 0642.1

In stock
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from 6 Pack Qty. €60.18/Pack Qty.
from 24 Pack Qty. €57.02/Pack Qty.

Product details

Sterile

Ready-to-use and sterile separation medium based on polysucrose 400, for efficient isolation of lymphocytes from human blood by density gradient centrifugation.
Density 1.077 g/ml.
Best alternative to Ficoll®.

  • Optimised for isolation of lymphocytes from non-coagulated human whole blood
  • Produces distinct, compact layers
  • Results in high recovery rates of viable cells
  • Maintains the representative ratio of B- to T lymphocytes
  • May be directly applied to all protocols using Ficoll®

ROTI®Sep 1077 human excels due to the superior separation power, forming very distinct, easy to pipet, lymphocyte layers during centrifugation. The ready to use solution is very easy in handling, resulting in high cell recovery rates, while cell viability and the representative ratio of B- to T lymphocytes are maintained. Osmolality as well as pH value are strictly kept in the physiological range.

Suitable for application with human non-coagulated peripheral blood. In all respective protocols originally using Ficoll® this reagent may be replaced by ROTI®Sep 1077 human without any adjustment.



ROTI®Sep 1077 human CELLPURE® sterile, ready-to-use, for density gradient centrifugation

0642_02
The figure depicts two specimens prepared with heparinic blood obtained from venous puncture. Density gradient centrifugation of 6 ml diluted whole blood (dilution in PBS 1:1.5), with 3 ml of ROTI®Sep 1077 human, 10 mins., 1000 g, no break.


Not a medical device / Not an IVD product
ROTI®Sep 1077 human
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Art. No. Pack Qty. Pack. Price Quantity
0642.1 100 ml plastic

€63.35

0642.2 500 ml plastic

€224.70

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Downloads / MSDS


Certificates of Analysis

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The following analysis certificates have been found:

Type analysis

Appearance of solutionclear, colourless
Density1,077 ±0,001 g/ml
pH value6.0-9.0
Osmolality325 ±35 mOsm/kg
Sterilitycomplies
Endotoxinstested