ROTIPHORESE®DNA sequencing system
ADR 6.1 III
One set is sufficient for 35 gels of 6 % (30 x 40 cm) with 40 % urea
excl. VAT. | 1 kit per Pack Qty.
Art. No. A431.1
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- For preparation of 4-20% sequencing gels
- Most simple handling of three optimised ready-to-use solutions
- No tedious dissolving of urea
- Prevents acrylamide dusts
- Reproducible, superior quality of gels
- In tried-and-tested ROTIPHORESE® quality
Convenient set containing three ready-made solutions for easy and fast casting of sequencing gels. Solutions are produced in ROTIPHORESE® quality and are perfectly matched - providing superior, reproducible quality and high sequence resolution.
In addition, the ROTIPHORESE®Sequencing system allows flexible adaptation to the desired gel variant, so that the amount of urea can be varied in addition to the acrylamide concentration. By using the ROTIPHORESE®sequencing gel buffer concentrate and ROTIPHORESE®Sequencing gel diluent contained in the set, gels with 50 % urea content are obtained. By adding defined quantities of ROTIPHORESE®10x TBE buffer or water, the urea content can be reduced to up to 40 %. "
Handling is most simple: Measure and mix certain amounts of all three components, add APS and TEMED, and cast the gel. We recommend using the Carl ROTH sequencing electrophoresis chamber (order no. AE28).
ROTIPHORESE®DNA sequencing system ROTIPHORESE® for DNA sequencing
• 1 l ROTIPHORESE®sequencing gel concentrate (Art. No. 3043.1) - ready-to-use, stabilised solution of 25% acrylamide/bisacrylamide 19:1 with 50% urea (8,3 M).
• 1 l ROTIPHORESE®sequencing gel diluent (Art. No. 3047.1) - 50% (g/v; 8,3 M) solution of urea in water.
• 250 ml ROTIPHORESE®sequencing gel buffer concentrate (Art. No. 3050.1) - 10x TBE with 50% urea (8,3 M).
• Detailed instructions for use are enclosed with the product.
- Subtotal: 0.00
|Order No.||Pack Qty.||Pack.||Packaging||Price||Quantity|
|A431.1||1 kit||glass||2 x 1 l, 1 x 250 ml||€113.85||
Available at short notice
Delivery date currently unknown
Acrylamide ia a strong nerve poison which has clearly proved to be carcinogenic and to change the genetic make-up during animal testing. Acrylamide is resorbed primarily through the skin. The formation of dust when working with the solid matter is particularly critical. A hazard which can be easily avoided by using the ROTIPHORESE® ready-to-use solution.
ROTIPHORESE® Acrylamide and -Bisacrylamide solutions from Carl ROTH have the highest purity level. They are produced from substances free from acrylic acid. Oxidation products are removed from the solution by a selective absorption process. A new formation of such and/or polymerisation of the solution is prevented by gaseous stabilisation of the solution.
In order to guarantee a high consistency of our ROTIPHORESE®-solutions, each batch undergoes electrophoretic testing.
Recommendation for Use of ROTIPHORESE® Ready-Made Gel Solutions
|Analysis of||Gel solution||Art. No.|
|Proteins/peptides >15 kDa, DNA fragments||Gel 30 (37,5:1)||3029.1|
|Gel 40 (37,5:1)||T802.1|
|Proteins/peptides <30 kDa and DNA oligonucleotides||Gel 40 (29:1)||A515.1|
|DNA sequences (manual sequencing)||ROTIPHORESE®DNA sequencing system||A431.1|
|Gel 40 (19:1)||3030.1|
|DNA sequences (short-run automat. sequencing)||NF solution 40 % (19:1)||A516.1|
|DNA sequences (long-run automat. sequencing)||NF solution 30 % (29:1)||A124.1|
|NF solution 40 % (29:1)||A121.1|