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ROTIPHORESE®Gel A, 1 l

ROTIPHORESE® 30 %, gas-stabilized
Icon_EVE-EUD Please consider that this product might be liable to some legal sanctions as for example to obtain an end user declaration or the prohibition of some applications. For details please do contact your counterpart in our sales department!
Examples of effect: Lead to damage to heath, cause irritation to eyes, skin or respiratory organs. Are fatal in larger quantities. Safety: As previously stated, in the event of skin irritation or contact with eyes, rinse with water or a suitable medium.
Examples of effect: Have an allergenic effect, carcinogenic, mutagenic, toxic to reproduction and cause developmental toxicity (reprotoxic) or damage to organs Safety: You must be well-informed before starting work with these substances; wear protective clothing and gloves, eye protection and mask or breathing protection.
Danger
H302-H315-H317-H319-H340-H350-H361f-H372
i harmful if swallowed, causes skin irritation, may cause an allergic skin reaction, causes serious eye irritation, may cause genetic defects, may cause cancer, suspected of damaging fertility, causes damage to organs (nervous system, testes) through prolonged or repeated exposure (if swallowed)
P201 P280 P301+P312 P302+P352 P305+P351+P338 P308+P313
i obtain special instructions before use, wear protective gloves/eye protection, IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell, IF ON SKIN: Wash with plenty of soap and water, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing, IF exposed or concerned: Get medical advice/attention
Pack Qty.
Pack.
Gel solution, PAGE gel solutions
Density (D) 1,023 g/cm³
Melting point (mp) 0 °C
Storage temp. +4 °C
WGK 3

Aqueous acrylamide stock solution for preparation of protein gels in combination with Rotiphorese® Gel B.
Gels with up to 20 % total acrylamide concentration can be produced.

Ready-to-use, gas-stabilized acrylamide ready-to-use solution. The ROTIPHORESE® gel s ...
Product details

€55.80/Pack Qty. 

excl. VAT. | 1 l per Pack Qty.

Art. No. 3037.1

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Product details


Ready-to-use, gas-stabilized acrylamide ready-to-use solution. The ROTIPHORESE® gel solution is very easy to use and enables reproducible and high-resolution gel electrophoresis.


ROTIPHORESE® gel solutions can be used for protein and nucleic acid separation in PAGE gels. The high-purity solutions can be combined with any buffer system. During sequencing, they facilitate the preparation of sequencing gels of consistent quality; our fluorescence-free gel solutions are recommended for the use of automatic sequencers.
The ROTIPHORESE® acrylamide and bisacrylamide solutions from Carl ROTH are produced only from acrylic acid-free substances and are strictly controlled in acrylamide content and pH. Oxidation products are removed from the solution by a selective absorption process. Their new formation and the polymerization of the solution are prevented by stabilizing the solution with inert gas.
In order to ensure a high lot-to-lot consistency of our ROTIPHORESE® solutions, each batch is subjected to an electrophoretic test.


Mechanism

PAGE solutions are always a mixture of acrylamide and bisacrylamide. Acrylamide forms linear polymers, whereas bisacrylamide crosslinks these linear polymers forming pores of a special size. The pore size may be altered by varying the total gel concentration (% T) and the percentage of the crosslink (% C).
With the ROTIPHORESE® stock solutions the desired T/C rate can be produced. You need an acrylamide stock solution (Gel A or Gel A-40) and a bisacrylamide stock solution (Gel B).


PAGE solutions are always a mixture of acrylamide and bisacrylamide. Acrylamide forms linear polymers, whereas bisacrylamide crosslinks these linear polymers forming pores of a special size. The pore size may be altered by varying the total gel concentration (% T) and the percentage of the crosslink (% C).
With the ROTIPHORESE® stock solutions the desired T/C rate can be produced. You need an acrylamide stock solution (Gel A or Gel A-40) and a bisacrylamide stock solution (Gel B).

For detailed instructions regarding gel preparation with ROTIPHORESE® Gel A, A-40 and B please see user manual and technical information brochure „PAGE Instructions“.


The right choice for each and every application
The large variety of different ROTIPHORESE® solutions provides you with the right product for each and every application. ROTIPHORESE® gel solution may be used both in protein and nucleic acid electrophoresis. ROTIPHORESE® ready-to-use solutions for manual sequencing facilitate the preparation of sequencing gels of a constant high quality - also suitable for automatic sequencers.

Safe
Acrylamide ia a strong nerve poison which has clearly proved to be carcinogenic and to change the genetic make-up during animal testing. Acrylamide is resorbed primarily through the skin. The formation of dust when working with the solid matter is particularly critical. A hazard which can be easily avoided by using the ROTIPHORESE® ready-to-use solution.

Superior quality
ROTIPHORESE® Acrylamide and -Bisacrylamide solutions from Carl ROTH have the highest purity level. They are produced from substances free from acrylic acid. Oxidation products are removed from the solution by a selective absorption process. A new formation of such and/or polymerisation of the solution is prevented by gaseous stabilisation of the solution.
In order to guarantee a high consistency of our ROTIPHORESE®-solutions, each batch undergoes electrophoretic testing.


Safe
Acrylamide ia a strong nerve poison which has clearly proved to be carcinogenic and to change the genetic make-up during animal testing. Acrylamide is resorbed primarily through the skin. The formation of dust when working with the solid matter is particularly critical. A hazard which can be easily avoided by using the ROTIPHORESE® ready-to-use solution.

Superior quality
ROTIPHORESE® Acrylamide and -Bisacrylamide solutions from Carl ROTH have the highest purity level. They are produced from substances free from acrylic acid. Oxidation products are removed from the solution by a selective absorption process. A new formation of such and/or polymerisation of the solution is prevented by gaseous stabilisation of the solution.
In order to guarantee a high consistency of our ROTIPHORESE®-solutions, each batch undergoes electrophoretic testing.



ROTIPHORESE®Gel A ROTIPHORESE® 30 %, gas-stabilized
Directions for use

Ready-to-use, stabilised 30 % acrylamide stock solution. In combination with ROTIPHORESE®Gel B (2 % bisacrylamide stock solution, Art. No. 3039) gels with up to 20 % total acrylamide concentration can be produced.


For detailed instructions regarding gel preparation with ROTIPHORESE®Gel A, A-40 and B please see our Technical Information Brochure „PAGE Instructions“.



ROTIPHORESE®Gel A
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3037.1 1 l glass €55.80
3037.2 250 ml glass €22.05
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Downloads


General information

Recommendation for Use of ROTIPHORESE® Ready-Made Gel Solutions

Analysis of Gel solution Art. No.
Proteins/peptides >15 kDa, DNA fragments Gel 30 (37,5:1) 3029.1
Gel 40 (37,5:1) T802.1
Proteins/peptides <30 kDa and DNA oligonucleotides Gel 40 (29:1) A515.1
DNA sequences (manual sequencing) ROTIPHORESE®DNA sequencing system A431.1
Gel 40 (19:1) 3030.1
DNA sequences (short-run automat. sequencing) NF solution 40 % (19:1) A516.1
DNA sequences (long-run automat. sequencing) NF solution 30 % (29:1) A124.1
NF solution 40 % (29:1) A121.1

Certificates of Analysis

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