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ROTIPHORESE®Gel B, 1 l

ROTIPHORESE® 2 %, gas-stabilized
Pack Qty.
Pack.
Gel solution, PAGE gel solutions
Density (D) 1 g/cm³
Boiling point (bp) 100 °C
Melting point (mp) 0 °C
Storage temp. +4 °C
WGK 1

Aqueous bisacrylamide stock solution for preparation of protein gels in combination with ROTIPHORESE®Gel A or Gel A-40.
Gels with up to 25 % total acrylamide concentration can be produced.

Ready-to-use, gas-stabilized bisacrylamide ready-to-use solution. The ROTIPHORESE® gel solution is very easy to use and enables reproducible and high-resolution gel electrophoresis.
Product details
Notes on the product

€51.50/Pack Qty. 

excl. VAT. | 1 l per Pack Qty.

Art. No. 3039.1

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Product details


Ready-to-use, gas-stabilized bisacrylamide ready-to-use solution. The ROTIPHORESE® gel solution is very easy to use and enables reproducible and high-resolution gel electrophoresis.


ROTIPHORESE® gel solutions can be used for protein and nucleic acid separation in PAGE gels. The high-purity solutions can be combined with any buffer system. During sequencing, they facilitate the preparation of sequencing gels of consistent quality; our fluorescence-free gel solutions are recommended for the use of automatic sequencers.
The ROTIPHORESE® acrylamide and bisacrylamide solutions from Carl ROTH are produced only from acrylic acid-free substances and are strictly controlled in acrylamide content and pH. Oxidation products are removed from the solution by a selective absorption process. Their new formation and the polymerization of the solution are prevented by stabilizing the solution with inert gas.
In order to ensure a high consistency of our ROTIPHORESE® solutions, each batch is subjected to an electrophoretic test.


Mechanism

PAGE solutions are always a mixture of acrylamide and bisacrylamide. Acrylamide forms linear polymers, whereas bisacrylamide crosslinks these linear polymers forming pores of a special size. The pore size may be altered by varying the total gel concentration (% T) and the percentage of the crosslink (% C).
With the ROTIPHORESE® stock solutions the desired T/C rate can be produced. You need an acrylamide stock solution (Gel A or Gel A-40) and a bisacrylamide stock solution (Gel B).


Acrylamide and Bisacrylamide Stock Solutions


PAGE solutions are always a mixture of acrylamide and bisacrylamide. Acrylamide forms linear polymers, whereas bisacrylamide crosslinks these linear polymers forming pores of a special size. The pore size may be altered by varying the total gel concentration (% T) and the percentage of the crosslink (% C).
With the ROTIPHORESE® stock solutions the desired T/C rate can be produced. You need an acrylamide stock solution (Gel A or Gel A-40) and a bisacrylamide stock solution (Gel B).

For detailed instructions regarding gel preparation with ROTIPHORESE® Gel A, A-40 and B please see user manual and technical information brochure “PAGE Instructions”.



ROTIPHORESE®Gel B ROTIPHORESE® 2 %, gas-stabilized
Directions for use

Ready-to-use, stabilised 2 % bisacrylamide stock solution. In combination with ROTIPHORESE®Gel A (30 % acrylamide stock solution, Art. No. 3037) or ROTIPHORESE®Gel A-40 (40 % acrylamide stock solution, Art. No. 7748) gels with up to 25 % total acrylamide concentration can be produced.


For detailed instructions regarding gel preparation with ROTIPHORESE®Gel A, A-40 and B please see our Technical Information Brochure „PAGE Instructions“.



ROTIPHORESE®Gel B
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3039.2 250 ml glass €21.40
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General information

Safe
Acrylamide ia a strong nerve poison which has clearly proved to be carcinogenic and to change the genetic make-up during animal testing. Acrylamide is resorbed primarily through the skin. The formation of dust when working with the solid matter is particularly critical. A hazard which can be easily avoided by using the ROTIPHORESE® ready-to-use solution.

Superior quality
ROTIPHORESE® Acrylamide and -Bisacrylamide solutions from Carl ROTH have the highest purity level. They are produced from substances free from acrylic acid. Oxidation products are removed from the solution by a selective absorption process. A new formation of such and/or polymerisation of the solution is prevented by gaseous stabilisation of the solution.
In order to guarantee a high consistency of our ROTIPHORESE®-solutions, each batch undergoes electrophoretic testing.


The right choice for each and every application
The large variety of different ROTIPHORESE® solutions provides you with the right product for each and every application. ROTIPHORESE® gel solution may be used both in protein and nucleic acid electrophoresis. ROTIPHORESE® ready-to-use solutions for manual sequencing facilitate the preparation of sequencing gels of a constant high quality - also suitable for automatic sequencers.


ROTIPHORESE®Gel 30 and Gel 40 are ready-to-use, gas-stabilised acrylamide solutions with different concentrations and with different ratios of acrylamide to bisacrylamide. ROTIPHORESE® ready-to-use solutions can be used directly.


Recommendation for Use of ROTIPHORESE® Ready-Made Gel Solutions

Analysis of Gel solution Art. No.
Proteins/peptides >15 kDa, DNA fragments Gel 30 (37,5:1) 3029.1
Gel 40 (37,5:1) T802.1
Proteins/peptides <30 kDa and DNA oligonucleotides Gel 40 (29:1) A515.1
DNA sequences (manual sequencing) ROTIPHORESE®DNA sequencing system A431.1
Gel 40 (19:1) 3030.1
DNA sequences (short-run automat. sequencing) NF solution 40 % (19:1) A516.1
DNA sequences (long-run automat. sequencing) NF solution 30 % (29:1) A124.1
NF solution 40 % (29:1) A121.1

Certificates of Analysis

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The following analysis certificates have been found:

Notes on the product

Filled under protective gas., Each batch is functionally tested for its suitability in electrophoresis.