ROTIPHORESE®Sequencing gel buffer concentrate
Boiling point (bp) 100 °C
Storage temp. +4 to +15 °C
One liter is sufficient for 160 gels (30 x 40 cm)
excl. VAT. | 250 ml per Pack Qty.
Art. No. 3050.1
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- For preparation of 4-20 % sequencing gels
- Most simple handling of three optimised ready-to-use solutions
- No tedious dissolving of urea
- Prevents acrylamide dusts
- Reproducible, superior quality of gels
- In tried-and-tested ROTIPHORESE® quality
Convenient set containing three ready-made solutions for easy and fast casting of sequencing gels. Solutions are produced in ROTIPHORESE® quality and are perfectly matched - providing superior, reproducible quality and high sequence resolution.
In addition, the ROTIPHORESE®Sequencing system allows flexible adaptation to the desired gel variant, so that the amount of urea can be varied in addition to the acrylamide concentration. By using the ROTIPHORESE®Sequencing gel buffer concentrate and ROTIPHORESE®Sequencing gel diluent contained in the set, gels with 50 % urea content are obtained. By adding defined quantities of ROTIPHORESE®10x TBE buffer or water, the urea content can be reduced to up to 40 %.
Handling is most simple: Measure and mix certain amounts of all three components, add APS and TEMED, and cast the gel. We recommend using the Carl ROTH sequencing electrophoresis chamber (Art. no. AE28).
ROTIPHORESE®Sequencing gel buffer concentrate ROTIPHORESE® 50 % (w/v, 8,3 M) in 10x TBE
Ready-to-use 10x TBE stock solution (1 M Tris borate, 20 mM EDTA buffer, pH 8,3) with 50 % urea .
For the preparation of polyacrylamide sequencing gels with 40-50 % urea (6,7-8,3 M).
Also part of the ROTIPHORESE®DNA sequencing system (Art. No. A431.1).
- Subtotal: 0.00
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Acrylamide ia a strong nerve poison which has clearly proved to be carcinogenic and to change the genetic make-up during animal testing. Acrylamide is resorbed primarily through the skin. The formation of dust when working with the solid matter is particularly critical. A hazard which can be easily avoided by using the ROTIPHORESE® ready-to-use solution.
ROTIPHORESE® Acrylamide and -Bisacrylamide solutions from Carl ROTH have the highest purity level. They are produced from substances free from acrylic acid. Oxidation products are removed from the solution by a selective absorption process. A new formation of such and/or polymerisation of the solution is prevented by gaseous stabilisation of the solution.
In order to guarantee a high consistency of our ROTIPHORESE®-solutions, each batch undergoes electrophoretic testing.
The right choice for each and every application
The large variety of different ROTIPHORESE® solutions provides you with the right product for each and every application. ROTIPHORESE® gel solution may be used both in protein and nucleic acid electrophoresis. ROTIPHORESE® ready-to-use solutions for manual sequencing facilitate the preparation of sequencing gels of a constant high quality - also suitable for automatic sequencers.
ROTIPHORESE®Gel 30 and Gel 40 are ready-to-use, gas-stabilised acrylamide solutions with different concentrations and with different ratios of acrylamide to bisacrylamide. ROTIPHORESE® ready-to-use solutions can be used directly.
Recommendation for Use of ROTIPHORESE® Ready-Made Gel Solutions
|Analysis of||Gel solution||Art. No.|
|Proteins/peptides >15 kDa, DNA fragments||Gel 30 (37,5:1)||3029.1|
|Gel 40 (37,5:1)||T802.1|
|Proteins/peptides <30 kDa and DNA oligonucleotides||Gel 40 (29:1)||A515.1|
|DNA sequences (manual sequencing)||ROTIPHORESE®DNA sequencing system||A431.1|
|Gel 40 (19:1)||3030.1|
|DNA sequences (short-run automat. sequencing)||NF solution 40 % (19:1)||A516.1|
|DNA sequences (long-run automat. sequencing)||NF solution 30 % (29:1)||A124.1|
|NF solution 40 % (29:1)||A121.1|
|1.0 M TRIS borate (pH 8.3), 20 mM EDTA., 50 % urea., distilled, deionized water., Remark: The product may crystallise. Can be redissolved by heating (max. 40 °C).|