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ROTIPHORESE®PAGE Matrix Buffer plus, 250 ml, 1 x 250 ml

ROTIPHORESE® 4x conc., for electrophoresis
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Icon_ready-to-use Ready-to-use products and reagents
Pack Qty.
Pack.
Packaging
SDS-PAGE buffer, Gel matrix buffer
Storage temp. +4 °C
Transport temp. cooled
WGK 1

Ready-made buffer solution for pouring of optimized SDS-PAGE gels.
One bottle of 250 ml is sufficient for pouring of approx. 100 mini gels.

€153.20/Pack Qty. 

excl. VAT. | 250 ml per Pack Qty.

Art. No. 8996.1

In stock
Additional transportation costs apply for this item.
from 6 Pack Qty. €145.54/Pack Qty.
from 24 Pack Qty. €137.88/Pack Qty.

Product details


  • Increases protein separation quality significantly
  • Superior band dissemination from 5 kDa to 250 kDa
  • Higher gel strength and longer shelf life
  • Compatible with all common gel loading and running buffers
  • Compatible with all common gel staining methods
  • Also suitable for 2D gels and prior to Western Blotting

ROTIPHORESE®PAGE Matrix Buffer plus is a 4x concentrated gel matrix buffer for enhanced protein separation and significantly higher gel strenth.


ROTIPHORESE®PAGE Matrix Buffer plus can be used for preparation of both stacking gel and resolving gel by simply replacing the Laemmli/TRIS-, or TRICIN-, buffer system. Even gels with stacking gels of only one acrylamide concentration, show a protein separation quality comparable to that of gradient gels. Band depiction is significantly enhanced compared to standard buffer systems.
ROTIPHORESE®PAGE Matrix Buffer plus also provides increased gel strength, making handling of the gels much more convenient, for instance during separation of the glass plates or during transport. Gel stability is increased, leading to longer shelf lifes of self-made gels. Gels prepared with ROTIPHORESE®PAGE Matrix Buffer plus may also very well be used for 2D protein analysis or for subsequent Western Blotting onto NC or PVDF membranes.



ROTIPHORESE®PAGE Matrix Buffer plus ROTIPHORESE® 4x conc., for electrophoresis

8996_05
Figure 4:
Stability of a 6 % PA gel.

8996_Banden
Figure 1 and 2:
Coomassie® stained gel and Western blot. HeLa extract on PVDF, detected via HRP chemoluminescence.
A: 10 % PAGE using ROTIPHORESE®PAGE Matrix buffer plus,
B: 12 % PAGE using standard Tris glycine SDS buffer.

8996_04
Figure 3:
Silver staining. HL-60 cell extract after 1D-IEF (pH 4-7) and 2D gel electrophoresis in a 12 % PA gel using ROTIPHORESE®PAGE Matrix buffer plus.


ROTIPHORESE®PAGE Matrix Buffer plus
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8996.1 250 ml plastic 1 x 250 ml

€153.20

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Downloads / MSDS


General information

Safe
Acrylamide ia a strong nerve poison which has clearly proved to be carcinogenic and to change the genetic make-up during animal testing. Acrylamide is resorbed primarily through the skin. The formation of dust when working with the solid matter is particularly critical. A hazard which can be easily avoided by using the ROTIPHORESE® ready-to-use solution.

Superior quality
ROTIPHORESE® Acrylamide and -Bisacrylamide solutions from Carl ROTH have the highest purity level. They are produced from substances free from acrylic acid. Oxidation products are removed from the solution by a selective absorption process. A new formation of such and/or polymerisation of the solution is prevented by gaseous stabilisation of the solution.
In order to guarantee a high consistency of our ROTIPHORESE®-solutions, each batch undergoes electrophoretic testing.


Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Type analysis

pH value6,9-7,1
Stability testcomplies
Remark: The product may crystallise. Can be redissolved by heating (max. 40 °C).