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ROTIPHORESE®PAGE Matrix Buffer plus, 250 ml, 1 x 250 ml

ROTIPHORESE® 4x konz., for electrophoresis
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Pack Qty.
Pack.
Packaging
Gel matrix buffer, SDS-Page buffer
Storage temp. +4 °C
Transport temp. cooled
WGK 1

Ready-made buffer solution for pouring of optimized SDS-PAGE gels.
One bottle of 250 ml is sufficient for pouring of approx. 100 mini gels.

Type analysis

€120.95/Pack Qty. 

excl. VAT. | 250 ml per Pack Qty.

Art. No. 8996.1

Delivery date currently unknown
from 6 Pack Qty. €114.90/Pack Qty.
from 24 Pack Qty. €108.86/Pack Qty.
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Product details


  • Increases protein separation quality significantly
  • Superior band dissemination from 5 kDa to 250 kDa
  • Higher gel strength and longer shelf life
  • Compatible with all common gel loading and running buffers
  • Compatible with all common gel staining methods
  • Also well suitable for 2D gels and prior to Western Blotting
  • 250 ml sufficient for approx. 100 mini gels

ROTIPHORESE®PAGE Matrix Buffer plus is a 4x concentrated gel matrix buffer for enhanced protein separation and significantly higher gel strenth.


ROTIPHORESE®PAGE Matrix Buffer plus can be used for preparation of both stacking gel and resolving gel by simply replacing the Laemmli/TRIS-, or TRICIN-, buffer system. Even gels with stacking gels of only one acrylamide concentration, show a protein separation quality comparable to that of gradient gels. Band depiction is significantly enhanced compared to standard buffer systems.
ROTIPHORESE®PAGE Matrix Buffer plus also provides increased gel strength, making handling of the gels much more convenient, for instance during separation of the glass plates or during transport. Gel stability is increased, leading to longer shelf lifes of self-made gels. Gels prepared with ROTIPHORESE®PAGE Matrix Buffer plus may also very well be used for 2D protein analysis or for subsequent Western Blotting onto NC or PVDF membranes.



ROTIPHORESE®PAGE Matrix Buffer plus ROTIPHORESE® 4x konz., for electrophoresis

8996_05
Figure 4:
Stability of a 6% PA gel.

8996_Banden
Figure 1 and 2:
Coomassie® stained gel and Western blot. HeLa extract on PVDF, detected via HRP chemoluminescence.
A: 10% PAGE using Rotiphorese®-PAGE Matrix buffer plus,
B: 12% PAGE using standard Tris glycine SDS buffer.

8996_04
Figure 3:
Silver staining. HL-60 cell extract after 1D-IEF (pH 4-7) and 2D gel electrophoresis in a 12% PA gel using Rotiphorese®-PAGE Matrix buffer plus.


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Analysis certificates

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The following analysis certificates have been found:

Type analysis

pH value6,9-7,1  
Stability testcomplies  
Remark: The product may crystallise. Can be redissolved by heating (max. 40 °C).

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