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ROTI®Pol TaqHY Mix (2x), 2 ml, 2 x 1 ml

2x conc., ready-to-use
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Pack Qty.
Master mix Taq DNA polymerase, recombinant, PCR master mix
Density (D) ~1 g/cm³
Boiling point (bp) 100 °C
Storage temp. -20 °C
Transport temp. cooled

For rapid high yield PCR amplifications.
PCR premix w/o primers and template, optimized for reactions of 25 µl volume.

For PCR. Recombinant, heat stable DNA polymerases from the thermophilic bacteria Thermus aquaticus.
Product details

€89.15/Pack Qty. 

excl. VAT. | 2 ml per Pack Qty.

Art. No. 1K33.1

In production
from 6 Pack Qty. €84.69/Pack Qty.
from 24 Pack Qty. €80.24/Pack Qty.

Product details

For PCR. Recombinant, heat stable DNA polymerases from the thermophilic bacteria Thermus aquaticus.

ROTI®Pol, the series of DNA polymerases is the optimal choice for all PCR cycling protocols, as being performed in, for instance, analysis of cloning efficiency, for gene fishing, in routine screening processes, educational assays and much more. In combination with our specially designed buffers, the ROTI®Pol DNA polymerases deliver specific and reproducible PCR amplification with a wide range of PCR templates.

ROTI®Pol TaqHY Mix (2x) 2x conc., ready-to-use

Optimized, pre-mixed 2x PCR solution containing the recombinant TaqHY DNA polymerase from the thermophilic bacterium Thermus aquaticus, dNTPs, MgCl2 and all other components required for PCR except primers and template DNA. ROTI®Pol TaqHY Mix (2x) is recommended for all PCR applications where very short cycles or high yield are required.

PCR assaying with ROTI®Pol TaqHY Mix (2x) master mix not only reduces contamination risks, but is also time-saving, highly reproducible and very easy to prepare. ROTI®Pol TaqHY Mix (2x) shows both an enhanced polymerase activity and an extremely time-saving, fast elongation rate.

Due to the optimized composition of the master mix, the TaqHY polymerase offers specific PCR amplification with good yield on a wide range of PCR templates. The yield achieved is significantly higher than that of conventional Taq polymerases, with shorter cycle times. ROTI®Pol TaqHY Mix (2x) is able to amplify PCR products up to 3 kb with genomic DNA, and is appropriate for use with pure DNA solutions, cDNA, and bacterial colonies as templates. The use of the colourless PCR Master mix is particularly recommended when direct fluorescence or absorbance readings are required. The TaqHY DNA polymerase included in the mastermix possesses a 5' →3' polymerase as well as a 5'-flap endonuclease activity and generates a 3'dA (adenine) overhang which can be used for TA cloning purposes.

The set contains

2 or 10 vials, respectively, with 1 ml each of ROTI®Pol TaqHY Mix (2x) containing TaqHY polymerase, 0.4 mM each dNTP, and 4 mM MgCl2 in 2x reaction buffer

Not a medical device / Not an IVD product
Technical Information
Application Fast PCR amplifications of GC-rich template DNA with high yield 
Amplicon ends 3'dA 
Master mix colourless 
Polymerase High yield / high performance Taq polymerase 
Use Fast PCR protocols and/or high yield, GC rich template DNA 
ROTI®Pol TaqHY Mix (2x)
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Art. No. Pack Qty. Pack. Packaging Price Quantity
1K33.1 2 ml plastic 2 x 1 ml


1K33.2 10 ml plastic 10 x 1 ml


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Downloads / MSDS

Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Type analysis

Endonuclease activitynone detected
Exonuclease activitynone detected
Suitability for PCR (400 bp)complies
Suitability for PCR (3 kb)complies
Selective specificity in PCRcomplies