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Proteinase K, 25 mg, 1 x 25 mg

≥30 U/mg, lyophilised
Icon_DNAse-free
Icon_RNase-free
Examples of effect: Lead to damage to heath, cause irritation to eyes, skin or respiratory organs. Are fatal in larger quantities. Safety: As previously stated, in the event of skin irritation or contact with eyes, rinse with water or a suitable medium.
Examples of effect: Have an allergenic effect, carcinogenic, mutagenic, toxic to reproduction and cause developmental toxicity (reprotoxic) or damage to organs Safety: You must be well-informed before starting work with these substances; wear protective clothing and gloves, eye protection and mask or breathing protection.
Danger
H315-H317-H319-H334-H335
i causes skin irritation, may cause an allergic skin reaction, causes serious eye irritation, may cause allergy or asthma symptoms or breathing difficulties if inhaled, may cause respiratory irritation
P260 P280 P342+P311
i do not breathe dust, wear protective gloves/eye protection, if experiencing respiratory symptoms: Call a POISON CENTER/doctor
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Endopeptidase
Storage temp. -20 °C
Transport temp. ambient temp.
WGK 1
CAS No. 39450-01-6
EG-Nr. 254-457-8

Recommended stock solution: 20 mg/ml in water. Storage in aliquots at -20 °C.
Art. No. 23NX.5: A The stock solution (20 mg/ml) can be prepared directly in the bottle.
Serine protease for degrading proteins in biological samples. Isolated from fungi (Tritirachium album).

Proteinase K (from Tritirachium album) is a non-specific protease of the serine protease family.
Proteinase K is used for the cleavage of proteins in nucleic acid preparations. It is mainly used in nucleic acid purification or for the removal of nucleases.
Proteinase K is active under a wide range of reaction conditions, including elevated temperatures and presence of SDS.
Product details

€26.80/Pack Qty. 

excl. VAT. | 25 mg per Pack Qty.

Art. No. 23NX.6

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Product details


Proteinase K (from Tritirachium album) is a non-specific protease of the serine protease family.
Proteinase K is used for the cleavage of proteins in nucleic acid preparations. It is mainly used in nucleic acid purification or for the removal of nucleases.
Proteinase K is active under a wide range of reaction conditions, including elevated temperatures and presence of SDS.


Directions for use

Foreign activity: RNAse and DNAse not detectable
Optimum temperature: +65 °C.
Activity at +65 °C is ca. 12 x higher than at +25 °C. Over +65 °C, inactivation due to denaturation.
Activators: Denaturating agents like SDS (0,5-1 %), urea.
Inhibitors: Inhibition with Hg2+-ions, DFP, PMSF and phenol. Not inhibited by EDTA, sulfhydryl reagents and trypsin or chymotryps ininhibitors.
Stability: pH 4.0-12.5. pH optimum: 8,0.
Also stable even when denaturing agents, e.g. SDS and urea are present.
Stabilisers: Ca2+-ions (1-5 mM) prevent autolysis.



Proteinase K ≥30 U/mg, lyophilised

Proteinase K is often used for cell and tissue disruption and for isolation and purification of nucleic acids.


Directions for use

The recommended working concentration: 50-100 µg/ml for protein and up to 2 mg/ml for tissue treatment.


Unit definition

One unit is defined as the amount of enzyme that hydrolyzes a defined casein substrate to 1 μM L-tyrosine per minute at 55 °C and pH 8.0.



Not a medical device / Not an IVD product
Proteinase K
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Art. No. Pack Qty. Pack. Packaging Price Quantity
23NX.1 100 mg glass 1 x 100 mg

€83.75

23NX.2 500 mg glass 1 x 500 mg

€317.15

23NX.3 1 g glass 1 x 1 g

€521.40

23NX.4 5 g glass 1 x 5 g

€2,090.90

23NX.5 500 mg glass in 30 ml glass vial

€342.95

23NX.6 25 mg glass 1 x 25 mg

€26.80

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Downloads / MSDS


General information

Enzyme: a neoclassical, Greek artificial word ενζυμου, énzymon, derived from εν-, en- (in-) and ζυμη, zýmé (yeast, sourdough, archaic)
Ferments: comes from the Latin fermentum (ferments, sourdough)

There are six classes in which all enzymes are classified according to the particular reaction they catalyse:

Oxidoreductases (catalyse redox reactions)

Transferases (transfer functional groups among substrates)

Hydrolases (cleave bonds via addition of water)

Lyases/Synthases (cleave or synthesise complex products out of basic substrates without cleavage of ATP)

Isomerases (transform chemical isomers)

Ligases/Synthetases (cleave or synthesise complex products out of basic substrates via cleavage of ATP)


Guarantee analysis

Appearancewhite powder
Specific activity≥30 U/mg
DNases, RNasesnot detected