Plasmid DNA pBR322
Transport temp. ambient temp.
pBR322, Acc. No. V01119
full lenght: 4361 bp
excl. VAT. | 50 µg per Pack Qty.
Art. No. X912.1
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The following plasmid DNA was prepared in low nuclease host bacteria and isolated through ion exchange chromatography. It is available in supercoiled form (ccc-form >95%) and, as a high-purity DNA (OD260/280 > 1,70), it is ideal for different applications:
- Controlling transformation efficiency
- Comparison with plasmid ccc-forms of unknown size
- Cloning DNA-fragments
- Restriction (Preparing DNA-length markers)
- Calibrating DNA-separating or DNA-preparing processes or equipment
- Blocking DNA-chips with plasmid DNA of a defined sequence
Plasmid DNA pBR322 lyophilized
Genomic DNA: <2 %
oc form: <3 %
ccc form: >95 %
Base pairs: 4361
The circular sequence was numbered in such a way that 0 lies in the middle of the singular EcoR l restriction site. The copy number of pBR322 is limited by the gene product of rop to 20 copies per cell. However, by adding chloroamphenicol (final concentration 170 µg/ml) to log-phase cultures and then incubating for 8 hours, the copy number can be increased considerably.
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