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Plasmid DNA pBR322

Pack Qty.
Storage temp. -20 °C
Transport temp. ambient temp.

High-purity plasmid-DNA of pBR322 for genetic engineering
pBR322, Acc. No. V01119
full lenght: 4361 bp

The following plasmid DNA was prepared in low nuclease host bacteria and isolated through ion exchan ...
Product details

€62.90/Pack Qty. 

excl. VAT. | 50 µg per Pack Qty.

Art. No. X912.1

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Product details

The following plasmid DNA was prepared in low nuclease host bacteria and isolated through ion exchange chromatography. It is available in supercoiled form (ccc-form >95%) and, as a high-purity DNA (OD260/280  > 1,70), it is ideal for different applications:

  • Controlling transformation efficiency
  • Comparison with plasmid ccc-forms of unknown size
  • Cloning DNA-fragments
  • Restriction (Preparing DNA-length markers)
  • Calibrating DNA-separating or DNA-preparing processes or equipment
  • Blocking DNA-chips with plasmid DNA of a defined sequence

Plasmid DNA pBR322 lyophilized

OD260/280: >1.70
Genomic DNA: <2 %
oc form: <3 %
ccc form: >95 %
Base pairs: 4361

The circular sequence was numbered in such a way that 0 lies in the middle of the singular EcoR l restriction site. The copy number of pBR322 is limited by the gene product of rop to 20 copies per cell. However, by adding chloroamphenicol (final concentration 170 µg/ml) to log-phase cultures and then incubating for 8 hours, the copy number can be increased considerably.

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