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Plasmid DNA pUC19

Pack Qty.
Storage temp. -20 °C
Transport temp. ambient temp.

High-purity plasmid-DNA of pUC19 for genetic engineering
pUC19c, Acc. No. L09137
full llength: 2686 bp

The following plasmid DNA was prepared in low nuclease host bacteria and isolated through ion exchan ...
Product details

€53.25/Pack Qty. 

excl. VAT. | 50 µg per Pack Qty.

Art. No. X911.1

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Product details

The following plasmid DNA was prepared in low nuclease host bacteria and isolated through ion exchange chromatography. It is available in supercoiled form (ccc-form >95%) and, as a high-purity DNA (OD260/280  > 1,70), it is ideal for different applications:

  • Controlling transformation efficiency
  • Comparison with plasmid ccc-forms of unknown size
  • Cloning DNA-fragments
  • Restriction (Preparing DNA-length markers)
  • Calibrating DNA-separating or DNA-preparing processes or equipment
  • Blocking DNA-chips with plasmid DNA of a defined sequence

Plasmid DNA pUC19 lyophilized

OD260/280: >1.70
Genomic DNA: <2 %
oc form: <3 %
ccc form: >95 %
Base pairs: 2686

pUC19 is a standard high-copy cloning vector for E. coli recombinants. The position of the Multi Cloning Site (MCS), which is inserted in frame into the LacZ-gene, enables a blue-white selection of insert containing plasmid DNA by α-complementation. The copy number of plasmids per cell depends on the temperature and is approx. 70-80 at 37 °C and over 200 at 42 °C.

Plasmid DNA pUC19
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