Plasmid DNA pUC19
Transport temp. ambient temp.
pUC19c, Acc. No. L09137
full llength: 2686 bp
excl. VAT. | 50 µg per Pack Qty.
Art. No. X911.1
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The following plasmid DNA was prepared in low nuclease host bacteria and isolated through ion exchange chromatography. It is available in supercoiled form (ccc-form >95%) and, as a high-purity DNA (OD260/280 > 1,70), it is ideal for different applications:
- Controlling transformation efficiency
- Comparison with plasmid ccc-forms of unknown size
- Cloning DNA-fragments
- Restriction (Preparing DNA-length markers)
- Calibrating DNA-separating or DNA-preparing processes or equipment
- Blocking DNA-chips with plasmid DNA of a defined sequence
Plasmid DNA pUC19 lyophilized
Genomic DNA: <2 %
oc form: <3 %
ccc form: >95 %
Base pairs: 2686
pUC19 is a standard high-copy cloning vector for E. coli recombinants. The position of the Multi Cloning Site (MCS), which is inserted in frame into the LacZ-gene, enables a blue-white selection of insert containing plasmid DNA by α-complementation. The copy number of plasmids per cell depends on the temperature and is approx. 70-80 at 37 °C and over 200 at 42 °C.
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