Technical Data Sheet
anti-Desmoglein 1/2 mouse monoclonal, DG 3.10, 0.2 ml
Bitte beachten: Abweichende Konditionen / Please note: Different conditions
Boiling point (bp) ~100 °C
Storage temp. +4 °C
Transport temp. ambient temp.
€130.65/Pack Qty.
excl. VAT. | 0.2 ml per Pack Qty.
Art. No. 1NP0.1
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Product details
This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.
- High epitope affinity
- Highly specific detection of the target protein
- Unconjugated
- Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence
anti-Desmoglein 1/2 mouse monoclonal, DG 3.10 50 μg/ml, monoclonal, affinity purified
DG 3.10 recognizes desmoglein 1 + 2 (desmosome-specific cadherins) in desmosome-bearing cells; does not react with cells containing plaque-bearing junctions of non-desmosomal type.
Tumors specifically detected: all epithelium-derived tumors, notably carcinomas, and meningiomas.
Polypeptide reacting: Mr 165 000 Desmoglein, 'band 3' polypeptide of the desmosomal complex. Epitope recognized has been localized at the repeating unit domain ('RUD') of the cytoplasmic C-terminal part of the polypeptide.
Reactivity on cultured cell lines: A-431, MDBK.
Immunocytochemistry (ICC): Assay dependent
Immunohistochemistry (IHC) - frozen: 1:10-1:100 (0.5-5 μg/ml)
Immunohistochemistry (IHC) - paraffin: 1:10-1:100 (0.5-5 μg/ml, microwave treatment recommended)
Western Blot (WB): 1:2000-1:4000 (12,5 ng/ml - 25 ng/ml)
| Application |
ICC/IF IHC WB |
| Host species | mouse |
| Clonality | monoclonal |
| Reactivity antibody |
rat cattle human |
| Purification | Affinity chromatography |
| Conjugate | uncojugated |
| Concentration | 50 µg/ml |
| Isotype | IgG1 |
| Uniprot ID AK | Q02413 (human), D3ZM39 (rat), Q03763 (bovine) |
| Clone | DG 3.10 |
| Target | 'band 3' polypeptide of isolated desmosomes from bovine muzzle epidermis |
- Subtotal: 0.00
| Art. No. | Pack Qty. | Pack. | Price | Quantity | |
|---|---|---|---|---|---|
| 1NP0.1 | 0.2 ml | plastic |
€130.65 |
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| 1NP0.2 | 1 ml | plastic |
€332.20 |
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- Subtotal: 0.00
Downloads / MSDS
General information
Secondary antibodies do not bind directly to the target molecule, but rather to the primary antibody. They are usually coupled with enzymes or fluorescent dyes so that the binding of the primary antibody can be made visible or measurable. In research, they serve as signal amplifiers and enable more sensitive detection, as several secondary antibodies can bind to one primary antibody. This makes them important tools in methods such as ELISA, Western blot or microscopy.
Primary antibodies are proteins that bind specifically to a particular antigen, i.e. a target molecule such as a protein or a structure within a cell. They are used in research to visualise specific molecules, isolate them or investigate their function. Typical applications include Western blotting, immunohistochemistry and flow cytometry. Due to their high specificity, primary antibodies enable the precise detection of biological processes.