Technical Data Sheet
anti-Desmoglein 2 mouse monoclonal, 10G11, 0.2 ml
Bitte beachten: Abweichende Konditionen / Please note: Different conditions
Boiling point (bp) ~100 °C
Storage temp. +4 °C
Transport temp. ambient temp.
€130.65/Pack Qty.
excl. VAT. | 0.2 ml per Pack Qty.
Art. No. 1NLT.1
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Fits to / Accessories
Product details
This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.
- High epitope affinity
- Highly specific detection of the target protein
- Unconjugated
- Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence
anti-Desmoglein 2 mouse monoclonal, 10G11 500 μg/ml, monoclonal, affinity purified
Antibody recognizes desmoglein 2 present in low concentrations in all epithelia and in tumors derived therefrom as well as in heart muscle and dendritic reticulum cells of lymphatic follicles. In basal epithelial cells desmoglein 2 expression is generally very low.
Polypeptide reacting: desmoglein 2 (1069aa; calculated mol. wt. 116,760)
Reactivity on cultured cell lines: many, including CaCo-2, HaCaT, MCF-7
Immunohistochemistry (IHC) - frozen: 1:100 (5 μg/ml, for better resolution preincubation (directly after fixation) with 0.05-0.2 % Triton X-100 for 5-10 min, depending on tissue type, is recommended (Schäfer S. et al., 1996))
Western Blot (WB): assay dependent
| Application |
IHC WB |
| Host species | mouse |
| Clonality | monoclonal |
| Reactivity antibody |
mouse rat |
| Purification | Affinity chromatography |
| Conjugate | uncojugated |
| Concentration | 500 µg/ml |
| Isotype | IgG1 |
| Uniprot ID AK | Q14126 (human) |
| Clone | 10G11 |
| Target | recombinant peptide (220aa of 2nd and 3rd extracellular repeat domain) |
- Subtotal: 0.00
| Art. No. | Pack Qty. | Pack. | Price | Quantity | |
|---|---|---|---|---|---|
| 1NLT.1 | 0.2 ml | plastic |
€130.65 |
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| 1NLT.2 | 1 ml | plastic |
€327.90 |
|
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- Subtotal: 0.00
Downloads / MSDS
General information
Secondary antibodies do not bind directly to the target molecule, but rather to the primary antibody. They are usually coupled with enzymes or fluorescent dyes so that the binding of the primary antibody can be made visible or measurable. In research, they serve as signal amplifiers and enable more sensitive detection, as several secondary antibodies can bind to one primary antibody. This makes them important tools in methods such as ELISA, Western blot or microscopy.
Primary antibodies are proteins that bind specifically to a particular antigen, i.e. a target molecule such as a protein or a structure within a cell. They are used in research to visualise specific molecules, isolate them or investigate their function. Typical applications include Western blotting, immunohistochemistry and flow cytometry. Due to their high specificity, primary antibodies enable the precise detection of biological processes.