Technical Data Sheet
anti-p53 Protein mouse monoclonal, Bp53.11, 1 ml
Bitte beachten: Abweichende Konditionen / Please note: Different conditions
Boiling point (bp) ~100 °C
Storage temp. +4 °C
Transport temp. ambient temp.
€284.90/Pack Qty.
excl. VAT. | 1 ml per Pack Qty.
Art. No. 1NNY.2
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Fits to / Accessories
Product details
This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.
- High epitope affinity
- Highly specific detection of the target protein
- Unconjugated
- Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence
anti-p53 Protein mouse monoclonal, Bp53.11 50 μg/ml, monoclonal, affinity purified
Excellent marker for wild-type and mutant forms of human p53 antigen.
Epitope recognized by Bp53.11: 20-SDLWKLLPENNV-31.
The antibody stains positively approx. 60% of investigated carcinoma of lung, breast, colon, stomach, esophagus, pancreas, urinary bladder and testis, head and neck tumors; T-cell Leukemia, non-Hodgkin-Lymphoma, melanoma, sarcoma.
Reactivity on cultured cell lines: Hela, MCF-7.
Immunocytochemistry (ICC): assay dependent
Immunohistochemistry (IHC) - frozen: 1:1,000-1:3,000 (17-50 ng/ml)
Immunohistochemistry (IHC) - paraffin: 1:1,000-1:3,000 (17-50 ng/ml, microwave treatment recommended)
Western Blot (WB): 1:2,000-1:10,000 (5-25 ng/ml)
| Application |
ICC/IF IHC WB |
| Host species | mouse |
| Clonality | monoclonal |
| Reactivity antibody | human |
| Purification | Affinity chromatography |
| Conjugate | uncojugated |
| Concentration | 50 µg/ml |
| Isotype | IgG2a |
| Uniprot ID AK | Q96A56 (human) |
| Clone | Bp53.11 |
| Target | Recombinant human p53 (transcription domain within the NH2 terminus) |
- Subtotal: 0.00
| Art. No. | Pack Qty. | Pack. | Price | Quantity | |
|---|---|---|---|---|---|
| 1NNY.1 | 0.2 ml | plastic |
€109.15 |
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| 1NNY.2 | 1 ml | plastic |
€284.90 |
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- Subtotal: 0.00
Downloads / MSDS
General information
Secondary antibodies do not bind directly to the target molecule, but rather to the primary antibody. They are usually coupled with enzymes or fluorescent dyes so that the binding of the primary antibody can be made visible or measurable. In research, they serve as signal amplifiers and enable more sensitive detection, as several secondary antibodies can bind to one primary antibody. This makes them important tools in methods such as ELISA, Western blot or microscopy.
Primary antibodies are proteins that bind specifically to a particular antigen, i.e. a target molecule such as a protein or a structure within a cell. They are used in research to visualise specific molecules, isolate them or investigate their function. Typical applications include Western blotting, immunohistochemistry and flow cytometry. Due to their high specificity, primary antibodies enable the precise detection of biological processes.