Jump to content Jump to navigation menu

anti-Strep-tag mouse monoclonal, C23.21, 25 µg

From  PROGEN
lyophilized, monoclonal, affinity purified
Icon_Abweichende-Konditionen Bitte beachten: Abweichende Konditionen / Please note: Different conditions
Related products
Pack Qty.
Pack.
Primary antibodies
Density (D) ~1 g/cm³
Boiling point (bp) 100 °C
Storage temp. +4 °C
Transport temp. ambient temp.

0.1 mg/ml after reconstitution with 250 μl / 1 ml PBS
Binding of Strep-tag proteins

Specific primary antibodies that recognise the most commonly used protein tags. The antibodies can be excellently used in cell or tissue biology studies, protein-protein interaction assays, post-translational modifications, expression dynamics or localisation studies, up to the production of analytical kits in laboratory or large scale.
Product details

€123.65/Pack Qty. 

excl. VAT. | 25 µg per Pack Qty.

Art. No. 1NLL.1

In stock
Delivery fast, simple and reliable!
from 6 Pack Qty. €117.47/Pack Qty.
from 24 Pack Qty. €111.28/Pack Qty.

Product details


Specific primary antibodies that recognise the most commonly used protein tags. The antibodies can be excellently used in cell or tissue biology studies, protein-protein interaction assays, post-translational modifications, expression dynamics or localisation studies, up to the production of analytical kits in laboratory or large scale.

  • Available for most commonly used protein tags (His, DDDDK, GST, HA, myc, GFP & Strep)
  • High affinity to the respective protein tag
  • Robust & highly specific detection of the fusion protein
  • Unconjugated
  • Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence


anti-Strep-tag mouse monoclonal, C23.21 lyophilized, monoclonal, affinity purified

The monoclonal C23.21 antibody recognizes Strep II. A Step-tag is commonly added to recombinant proteins and can be used for detection or purification of the tagged protein.


Application

Western Blot (WB): 1:5,000-1:10,000 (0.02-0.01 μg/ml)


1NLL
Western blot analysis of E. coli lysate containing Strep-tagged protein with anti-Strep-tag antibody. Western blot analysis was performed on 80 ng, 40 ng, or 10 ng of E. coli lysate containing Strep-tagged protein. Cells were lysed with SDS sample buffer. The PVDF membrane was blocked with 5% dry milk in PBST for 1 h at RT. The primary antibody anti-Strep-tag mouse monoclonal, C23.21 (Cat. No. 910STRL) was diluted in blocking buffer (antibody concentration 0.02 μg/ml) and incubated for 1 h at RT. The secondary antibody goat anti-mouse IgG polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.


Not a medical device / Not an IVD product
Technical Information
Application WB 
Host species mouse 
Clonality monoclonal 
Purification Affinity chromatography 
Conjugate uncojugated 
Isotype IgG1 
Clone C23.21 
Target Step-Tag 
anti-Strep-tag mouse monoclonal, C23.21
Selected quantity:   0
  1. Subtotal:  0.00
Art. No. Pack Qty. Pack. Price Quantity
1NLL.1 25 µg plastic

€123.65

In stock
Available (note delivery time)
In procurement
No longer available
Delivery date currently unknown
Selected quantity:   0
  1. Subtotal:  0.00

Downloads / MSDS

General information

Secondary Antibodies

Secondary antibodies do not bind directly to the target molecule, but rather to the primary antibody. They are usually coupled with enzymes or fluorescent dyes so that the binding of the primary antibody can be made visible or measurable. In research, they serve as signal amplifiers and enable more sensitive detection, as several secondary antibodies can bind to one primary antibody. This makes them important tools in methods such as ELISA, Western blot or microscopy.


Primary Antibodies

Primary antibodies are proteins that bind specifically to a particular antigen, i.e. a target molecule such as a protein or a structure within a cell. They are used in research to visualise specific molecules, isolate them or investigate their function. Typical applications include Western blotting, immunohistochemistry and flow cytometry. Due to their high specificity, primary antibodies enable the precise detection of biological processes.


Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found: