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anti-Synaptophysin mouse monoclonal, SY38, 1 ml

From  PROGEN
50 μg/ml, monoclonal, affinity purified
Icon_Abweichende-Konditionen Bitte beachten: Abweichende Konditionen / Please note: Different conditions
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Pack Qty.
Pack.
Antibody, Primary antibodies
Density (D) ~1 g/cm³
Boiling point (bp) ~100 °C
Storage temp. +4 °C
Transport temp. ambient temp.

Tumour marker neuronal/adrenal

This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.
Product details

€284.90/Pack Qty. 

excl. VAT. | 1 ml per Pack Qty.

Art. No. 1NN3.2

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from 6 Pack Qty. €270.66/Pack Qty.
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Product details


This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.

  • High epitope affinity
  • Highly specific detection of the target protein
  • Unconjugated
  • Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence


anti-Synaptophysin mouse monoclonal, SY38 50 μg/ml, monoclonal, affinity purified

SY 38 represents an excellent marker for several neuroendocrine, neuronal and adrenal tumors. Neuronal and adrenal tumors such as pheochromocytomas, paragangliomas, neuroblastomas, ganglioneuroblastomas.

Neuroendocrine tumors of epithelial origin: Pancreatic islet cell carcinoma, bronchial and gastrointestinal carcinoids, medullary carcinoma of thyroid.

Polypeptide reacting: 38 kDa transmembrane glycoprotein of presynaptic vesicles.

Tested cultured cell lines: rat PC-12 cell line.


Application

Immunocytochemistry (ICC): assay dependent
Immunohistochemistry (IHC) - frozen: 1:50-1:200 (0.25-1 μg/ml, no protease treatment)
Immunohistochemistry (IHC) - paraffin: 1:50-1:200 (0.25-1 μg/ml, microwave treatment recommended, no protease treatment)
Western Blot (WB): 1:500-1:1,000 (0.05-0.1 μg/ml)


1NN3_01
Mouse brain (courtesy of J. Heß, University Hospital Heidelberg)
1NN3_03
Western blot analysis of mouse brain lysate with anti-Synaptophysin antibody. Western blot analysis was performed on 20 μg mouse brain lysate. The PVDF membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-Synaptophysin mouse monoclonal, SY38 (Cat. No. 690012) was diluted in blocking buffer (antibody concentration 0.05 μg/ml) and incubated for 1 h at RT. The secondary antibody anti-mouse, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.
1NN3_04
Western blot analysis of rat Ins-1 lysate with anti-Synaptophysin antibody. Western blot analysis was performed on 20 μg rat Ins-1 lysate. The PVDF membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-Synaptophysin mouse monoclonal, SY38 (Cat. No. 690012) was diluted in blocking buffer (antibody concentration 0.1 μg/ml) and incubated for 1 h at RT. The secondary antibody anti-mouse, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.


Not a medical device / Not an IVD product
Technical Information
Application ICC/IF 
IHC 
WB 
Host species mouse 
Clonality monoclonal 
Reactivity antibody rat 
cattle 
mouse 
human 
Purification Affinity chromatography 
Conjugate uncojugated 
Concentration 50 µg/ml 
Isotype IgG1 
Uniprot ID AK P20488 (bovine), P08247 (human), Q62277 (mouse), P07825 (rat) 
Clone SY38 
Target Synaptophysin 
anti-Synaptophysin mouse monoclonal, SY38
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1NN3.1 0.2 ml plastic

€109.15

1NN3.2 1 ml plastic

€284.90

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Downloads / MSDS

General information

Secondary Antibodies

Secondary antibodies do not bind directly to the target molecule, but rather to the primary antibody. They are usually coupled with enzymes or fluorescent dyes so that the binding of the primary antibody can be made visible or measurable. In research, they serve as signal amplifiers and enable more sensitive detection, as several secondary antibodies can bind to one primary antibody. This makes them important tools in methods such as ELISA, Western blot or microscopy.


Primary Antibodies

Primary antibodies are proteins that bind specifically to a particular antigen, i.e. a target molecule such as a protein or a structure within a cell. They are used in research to visualise specific molecules, isolate them or investigate their function. Typical applications include Western blotting, immunohistochemistry and flow cytometry. Due to their high specificity, primary antibodies enable the precise detection of biological processes.


Certificates of Analysis

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