ROTI®Cell MEM-AA solution
Boiling point (bp) ~100 °C
Melting point (mp) ~0 °C
Storage temp. +4 °C
Transport temp. cooled
Solution in water.
Dilution 1:50 in cell culture medium. End concentration per amino acid conforms to EMEM.
excl. VAT. | 100 ml per Pack Qty.
Art. No. 0647.1
ROTI®Cell MEM-AA solution CELLPURE® 50x conz., sterile, w/o glutamine
ROTI®Cell MEM-AA solution consists of a pre-formulated mixture of 12 essential amino acids in water perfectly suited for culturing of most eukaryotic cells. Since cells are not able to synthesize essential amino acids by themselves, those have to be supplemented externallyd. A 1:50 dilution of ROTI Cell MEM-AA solution in cell culture medium will give a final concentration for each amino acid corresponding to the amount in Eagle's Minimum Essential Medium.
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Downloads / MSDS
Our amino acids are of the highest purity and suitable for a broad range of applications in biochemistry. Besides the natural occurring L-amino acids, we offer a selection of unnatural D-amino acids and DL-amino acids.
In addition to the various nutrients in the medium, the pH value also plays a very important role in cell culture. The physiological pH for almost all mammalian cells is 7.4. Deviating from this, it has been observed that certain transformed cell lines prefer a pH between 7.0 and 7.4 and certain fibroblasts prefer a pH between 7.4 and 7.7. In any case, pH fluctuations should be avoided and the pH should be maintained as well as possible. That is why a buffer system in media is necessary. The most important buffer system in the body is the carbonic acid-carbonate buffer system. Accordingly, almost all cell culture media contain bicarbonate (NaHCO3). Bicarbonate alone leads to a strong pH increase of the medium, so that NaHCO3-buffered media must always be used with CO2 incubators. Depending on the NaHCO3 concentration in the medium, different CO2 environments are necessary to ensure a pH of about 7.4. For this reason, you should not work outside of the CO2 incubator for too long. If this is necessary, a HEPES buffer should be used in addition to the NaHCO3, which contributes as a second buffer system to stabilize the pH value.
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