Technical Data Sheet
ROTI®Cell MEM-NEAA solution
Storage temp. +4 °C
Transport temp. ambient temp.
Solution in water.
Dilution 1:100 in cell culture medium. End concentration per amino acid 0,1 mM.
excl. VAT. | 100 ml per Pack Qty.
Art. No. 9185.1
ROTI®Cell MEM-NEAA solution CELLPURE® 100x conc., 10 mM (each), sterile
ROTI®Cell MEM-NEAA solution consists of a mixture of 7 non-essential amino acids in water. Enrichment of ROTI®Cell Eagle’s MEM Medium (with Earle’s or Hanks’ salts) with non-essential amino acids enables culturing of fastidious cells and cell lines, hereby broadening the utility of this medium. Dilution by 1:100 of ROTI®Cell MEM-NEAA solution yields a final concentration 0.1 mM of each component.
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Our amino acids are of the highest purity and suitable for a broad range of applications in biochemistry. Besides the natural occurring L-amino acids, we offer a selection of unnatural D-amino acids and DL-amino acids.
In addition to the various nutrients in the medium, the pH value also plays a very important role in cell culture. The physiological pH for almost all mammalian cells is 7.4. Deviating from this, it has been observed that certain transformed cell lines prefer a pH between 7.0 and 7.4 and certain fibroblasts prefer a pH between 7.4 and 7.7. In any case, pH fluctuations should be avoided and the pH should be maintained as well as possible. That is why a buffer system in media is necessary. The most important buffer system in the body is the carbonic acid-carbonate buffer system. Accordingly, almost all cell culture media contain bicarbonate (NaHCO3). Bicarbonate alone leads to a strong pH increase of the medium, so that NaHCO3-buffered media must always be used with CO2 incubators. Depending on the NaHCO3 concentration in the medium, different CO2 environments are necessary to ensure a pH of about 7.4. For this reason, you should not work outside of the CO2 incubator for too long. If this is necessary, a HEPES buffer should be used in addition to the NaHCO3, which contributes as a second buffer system to stabilize the pH value.