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ROTI®Cell MEM-NEAA solution

CELLPURE® 100x conc., 10 mM (each), sterile
Icon_Steril Sterile products
Sterile
Pack Qty.
Pack.
MEM-NEAA solution, Non-essential amino acids, Amino acids, non-essential
Boiling point (bp) ~100 °C
Storage temp. +4 °C
Transport temp. ambient temp.
WGK 1

For cell culture
Solution in water.
Dilution 1:100 in cell culture medium. End concentration per amino acid 0,1 mM.

ROTI®Cell MEM-NEAA solution consists of a mixture of 7 non-essential amino acids in water. Enrichment of ROTI®Cell Eagles MEM Medium (with Earles or Hanks salts) with non-essential amino acids enables culturing of fastidious cells and cell lines, hereby broadening the utility of this medium. Dilution by 1:100 of ROTI®Cell MEM-NEAA solution yields a final concentration 0.1 mM of each component.
Product details
€25.70 /Pack Qty.

€19.35/Pack Qty.  Campaign price!

excl. VAT. | 100 ml per Pack Qty.

Art. No. 9185.1

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Product details

Sterile


ROTI®Cell MEM-NEAA solution CELLPURE® 100x conc., 10 mM (each), sterile

ROTI®Cell MEM-NEAA solution consists of a mixture of 7 non-essential amino acids in water. Enrichment of ROTI®Cell Eagles MEM Medium (with Earles or Hanks salts) with non-essential amino acids enables culturing of fastidious cells and cell lines, hereby broadening the utility of this medium. Dilution by 1:100 of ROTI®Cell MEM-NEAA solution yields a final concentration 0.1 mM of each component.



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Downloads / MSDS


General information

Amino acids

Our amino acids are of the highest purity and suitable for a broad range of applications in biochemistry. Besides the natural occurring L-amino acids, we offer a selection of unnatural D-amino acids and DL-amino acids.


The buffer system in cell culture media

In addition to the various nutrients in the medium, the pH value also plays a very important role in cell culture. The physiological pH for almost all mammalian cells is 7.4. Deviating from this, it has been observed that certain transformed cell lines prefer a pH between 7.0 and 7.4 and certain fibroblasts prefer a pH between 7.4 and 7.7. In any case, pH fluctuations should be avoided and the pH should be maintained as well as possible. That is why a buffer system in media is necessary. The most important buffer system in the body is the carbonic acid-carbonate buffer system. Accordingly, almost all cell culture media contain bicarbonate (NaHCO3). Bicarbonate alone leads to a strong pH increase of the medium, so that NaHCO3-buffered media must always be used with CO2 incubators. Depending on the NaHCO3 concentration in the medium, different CO2 environments are necessary to ensure a pH of about 7.4. For this reason, you should not work outside of the CO2 incubator for too long. If this is necessary, a HEPES buffer should be used in addition to the NaHCO3, which contributes as a second buffer system to stabilize the pH value.


Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Type analysis

pH value3,1-3,7
Osmolalitytested
Endotoxins≤15,0 EU/ml
Sterilitycomplies