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ROTI®-Mark WESTERN Set, 0.5 ml

set of protein marker and antibody for gel and Western-Blot
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Examples of effect: Lead to damage to heath, cause irritation to eyes, skin or respiratory organs. Are fatal in larger quantities. Safety: As previously stated, in the event of skin irritation or contact with eyes, rinse with water or a suitable medium.
warning
H319
i causes serious eye irritation
P305+P351+P338 P337+P313
i IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing, if eye irritation persists: Get medical advice/attention
Pack Qty.
Pack.
Boiling point (bp) 100 °C
Storage temp. -20 °C
WGK 1

Size range: 10-120 kD
Number of protein bands: 8
Number of lanes: approx. 100 or 10 (mini gels)

Finely graded protein marker for medium size range and for detection in gel and on the Western-Blot  ...
Product details

€321.45/Pack Qty. 

excl. VAT. | 0.5 ml per Pack Qty.

Art. No. 0947.1

Not available
from 6 Pack Qty. €305.38/Pack Qty.
from 24 Pack Qty. €289.30/Pack Qty.
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Product details



ROTI®-Mark WESTERN Set set of protein marker and antibody for gel and Western-Blot

Finely graded protein marker for medium size range and for detection in gel and on the Western-Blot membrane. The marker itself consists of unstained proteins and therefore enables correct size evaluation in gel (left figure). The marker proteins are solubilised in a non-reducing buffer acc. to Laemmli.
The marker-specific antibody Roti®-Mark WESTERN HRP- conjugate (rabbit) enables the marker to be detected directly in Western- Blot and stained through chemoluminescence or colour reaction. Antibody incubation is performed in parallel with the incubation of your protein- specific antibodies and therefore saves any additional work and expense. The marker bands are also automatically stained (right figure) during subsequent detection by horseradish-peroxidase.
Consequently you will obtain the correct ladder size directly on your printout, X-ray film or membrane, ruling out the possibility of any errors through inaccurate transfer of the bands.


User information

Roti®-Mark WESTERN Marker can be bought seperately under Art. No. 0948.1.


Left hand fig.: 12 % minigel, 10 μl and 5 μl marker solution, respectively.

Right hand fig.: Chemoluminescence detection via Roti®-Lumin, Art. No. P078.1. Proteins were run in a 12 % minigel and transferred onto a Roti®-PVDF-Membran. 5 μl Roti®-Mark WESTERN Marker and Roti®-Mark WESTERN HRP conjugate diluted by 1:2.500.

0947_Bande_ai


ROTI®-Mark WESTERN Set
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0947.2 0.1 ml plastic €40.75
0947.1 0.5 ml plastic €321.45
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