ROTI®-Mark WESTERN Set, 0.5 ml
Storage temp. -20 °C
Number of protein bands: 8
Number of lanes: approx. 100 or 10 (mini gels)
excl. VAT. | 0.5 ml per Pack Qty.
Art. No. 0947.1
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ROTI®-Mark WESTERN Set set of protein marker and antibody for gel and Western-Blot
Finely graded protein marker for medium size range and for detection in gel and on the Western-Blot membrane. The marker itself consists of unstained proteins and therefore enables correct size evaluation in gel (left figure). The marker proteins are solubilised in a non-reducing buffer acc. to Laemmli.
The marker-specific antibody Roti®-Mark WESTERN HRP- conjugate (rabbit) enables the marker to be detected directly in Western- Blot and stained through chemoluminescence or colour reaction. Antibody incubation is performed in parallel with the incubation of your protein- specific antibodies and therefore saves any additional work and expense. The marker bands are also automatically stained (right figure) during subsequent detection by horseradish-peroxidase.
Consequently you will obtain the correct ladder size directly on your printout, X-ray film or membrane, ruling out the possibility of any errors through inaccurate transfer of the bands.
Roti®-Mark WESTERN Marker can be bought seperately under Art. No. 0948.1.
Left hand fig.: 12 % minigel, 10 μl and 5 μl marker solution, respectively.
Right hand fig.: Chemoluminescence detection via Roti®-Lumin, Art. No. P078.1. Proteins were run in a 12 % minigel and transferred onto a Roti®-PVDF-Membran. 5 μl Roti®-Mark WESTERN Marker and Roti®-Mark WESTERN HRP conjugate diluted by 1:2.500.
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