ROTI®Mark BICOLOR, 100 µl
Storage temp. -20 °C
Transport temp. ambient temp.
Number of protein bands: 9
Number of lanes/ml: approx. 200 (mini gels)
For direct application to the gel.
excl. VAT. | 100 µl per Pack Qty.
Art. No. 2806.1
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Perfect separation behaviour in the gel. Protein markers from Carl ROTH are acylated and pre-reduced, for optimal running behaviour in the gel. For each batch, intensity of bands is optimised and electrophoretically tested. All protein markers are offered as ready-to-use solutions which may be applied directly to the gel.
ROTI®Mark BICOLOR ready-to-use, pre-dyed
Protein marker including bicolour-prestained proteins of 17 up to 245 kDa. In order to visualise separation during the gel run and for better orientation in the gel, two proteins of approx. 25 and 55 kDa have been prestained in red in addition to the blue coloured bands. All proteins excel by having a very even running behaviour and form distinct bands.
ROTI®Mark BI-PINK is an ideal size marker for controlling the gel run during SDS-PAGE and for reliable evaluation of transfer efficiency during Western Blot.
Fig.: 4-20 % gradient SDS-PAGE (Tris)
Fig. 2: ROTI®Mark BICOLOR in Western Blotting. 5 µl marker solution and 20 µg protein extract (Caki-1) in a 10 % SDS-PAGE with ROTI®Load 1. Tank blotted onto Nitrocellulose with tris/glycin buffer acc. to Towbin (with 0.1 % SDS, 10 % methanol). Ponceau control staining after transfer.
With kind permission M. Schlosser, Fries group, Institute of Pathology, University Hospital of Köln.
|Number of protein bands||9|
|Amount of traces||~200/ml|
|Size range||17-245 kDa|
|Detection in western blot||no detection|
|Reference bands||25 kD, 55 kD pink|
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|Order No.||Pack Qty.||Pack.||Price||Quantity|
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