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ROTI®Quant universal, 500 assays (cuvettes)

for biochemistry
Examples of effect: Damage metals and burn body tissues; may cause serious eye damage. Safety: Avoid contact; wear safety spectacles and gloves. In the event of contact with eyes and skin, rinse with water.
Examples of effect: Are harmful to aquatic organisms, toxic or highly toxic, acute or with long-term effects. Safety: To be disposed of as hazardous waste only, do not allow into the environment under any circumstances.
Danger
H315-H318-H411
i causes skin irritation, causes serious eye damage, toxic to aquatic life with long lasting effects
P273 P280 P305+P351+P338 P310
i avoid release to the environment, wear protective gloves/eye protection, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing, immediately call a doctor
Pack Qty.
Pack.
Packaging
Biuret solutions
Density (D) 1,1 g/cm³
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
ADR 9 III
WGK 2
UN-Nr. 3082

Kit contents are adequate for 500/200 preparations in photometer cuvettes or 5000/2000 preparations in microtiter plates.
For protein quantitation (PCA enhanced Biuret reaction). High tolerance against detergents, salts and alcohols.

Colorimetric test for detecting and quantifying soluble proteins. Universally applicable.
Product details

€241.90/Pack Qty. 

excl. VAT. | 1 kit per Pack Qty.

Art. No. 0120.1

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from 6 Pack Qty. €229.80/Pack Qty.
from 24 Pack Qty. €217.71/Pack Qty.

Product details


Colorimetric test for detecting and quantifying soluble proteins. Universally applicable.

  • Equals the well-known BCA assay
  • Very easy to handle
  • Highly sensitive measurement up to 0.5 µg protein
  • Linear measuring range from 0.5 to 200 µg protein
  • Can be used in 1-ml-scales and in microtiter plates (can be automated)
  • Measurement with standard filters (492 nm wave length) or at 503 nm wave length
  • Accelerated reaction and increased sensitivity in mild heat

The special reagent formula effects a high tolerance against interfering factors such as detergents, alcohol and buffer salts, thus making the kit a universal tool for measuring proteins in almost every solution.


Mechanism

The basic mechanism, a PCA enhanced Biuret reaction, is identical with the one used for the well known BCA protein assay. Its result, however, is a bright blue colour which may be measured with standard filters of approx. 500 nm wave length.
ROTI®Quant universal is based on a biuret reaction in combination with a highly specific colorimetric enhancer reaction. The Cu++ ions of reagent 2 are reduced to Cu+ ions in the alkaline reaction media of the test. The colour development of the copper-specific enhancer reaction is directly proportional to the concentration of the protein which is present.
The resulting purple-coloured copper complex exhibits a strong light absorption with a maximum of 503 nm, although the wide maximum of the absorption curve also permits highly sensitive and no-loss measurements at 492 nm. The reaction does not form a true end point measurement: the colour develops slowly at room temperature, this, however, can be accelerated in heat. High sensitivity, therefore, can be achieved even after a short time.



ROTI®Quant universal for biochemistry
Mechanism


The kit contains

500/200 ml ROTI®Quant universal reagent 1 (Art. No. 0118) and 40/16 ml ROTI®Quant universal reagent 2 (Art. No. 0119).
Contents of this Kit may not be bought separately.

0120_Diagramm_A
Fig. A: ROTI®Quant universal, standard curve with BSA (bovine serum albumin). Blank-value corrected. Measurement of 100 µl each protein solution.
0120_Diagramm_B
Fig. B: Independence of ROTI®Quant-universal-assay of detergents and other additives, examples. Blank-value corrected. Measurement of 100 µl each protein solution.
(With kind permission of Dr. Angelika Böttger, Zoological Institute, Ludwig-Maximilians-University Munich)
0120_Diagramm_D
Fig. D: Independence of ROTI®Quant-universal assay of SDS. Microassay in microtiterplates (60 min, 37 °C).


Technical Information
Detection Biuret reaction/PCA (equals BCA) 
ROTI®Quant universal
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Art. No. Pack Qty. Pack. Packaging Price Quantity
0120.1 1 kit plastic 500 assays (cuvettes)

€241.90

0120.2 1 kit plastic 200 assays (cuvettes)

€117.20

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Downloads / MSDS


Certificates of Analysis

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Notes on the product

Tested for protein gel staining.