ROTI®Quant universal, 500 assays (cuvettes)
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
ADR 9 III
WGK 2
UN-Nr. 3082
Kit contents are adequate for 500/200 preparations in photometer cuvettes or 5000/2000 preparations in microtiter plates.
€151.15/Pack Qty. Campaign price!
excl. VAT. | 1 kit per Pack Qty.
Art. No. 0120.1
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Product details
Colorimetric test for detecting and quantifying soluble proteins. Universally applicable.
- Equals the well-known BCA assay
- Very easy to handle
- Highly sensitive measurement up to 0.5 µg protein
- Linear measuring range from 0.5 to 200 µg protein
- Can be used in 1-ml-scales and in microtiter plates (can be automated)
- Measurement with standard filters (492 nm wave length) or at 503 nm wave length
- Accelerated reaction and increased sensitivity in mild heat
The special reagent formula effects a high tolerance against interfering factors such as detergents, alcohol and buffer salts, thus making the kit a universal tool for measuring proteins in almost every solution.
The basic mechanism, a PCA enhanced Biuret reaction, is identical with the one used for the well known BCA protein assay. Its result, however, is a bright blue colour which may be measured with standard filters of approx. 500 nm wave length.
ROTI®Quant universal for biochemistry
ROTI®Quant universal is based on a biuret reaction in combination with a highly specific colorimetric enhancer reaction. The Cu++ ions of reagent 2 are reduced to Cu+ ions in the alkaline reaction media of the test. The colour development of the copper-specific enhancer reaction is directly proportional to the concentration of the protein which is present. The resulting purple-coloured copper complex exhibits a strong light absorption with a maximum of 503 nm, although the wide maximum of the absorption curve also permits highly sensitive and no-loss measurements at 492 nm. The reaction does not form a true end point measurement: the colour develops slowly at room temperature, this, however, can be accelerated in heat. High sensitivity, therefore, can be achieved even after a short time.
albumin). Blank-value corrected. Measurement of 100 µl each protein
solution.
Fig. B: Independence of ROTI®Quant-universal-assay of detergents and other additives, examples. Blank-value corrected. Measurement of 100 µl each protein solution.
(With kind permission of Dr. Angelika Böttger, Zoological Institute, Ludwig-Maximilians-University Munich)
500/200 ml ROTI®Quant universal reagent 1 (Art. No. 0118) and 40/16 ml ROTI®Quant universal reagent 2 (Art. No. 0119).
Contents of this Kit may not be bought separately.
| Detection | Biuret reaction/PCA (equals BCA) |
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| Order No. | Pack Qty. | Pack. | Packaging | Price | Quantity | |
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| 0120.1 | 1 kit | plastic | 500 assays (cuvettes) | €151.15 |
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| 0120.2 | 1 kit | plastic | 200 assays (cuvettes) | €71.90 |
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Certificates of Analysis
Notes on the product
| Tested for protein gel staining. |