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ROTI®Quant universal, 500 assays (cuvettes)

for biochemistry
Examples of effect: Damage metals and burn body tissues; may cause serious eye damage. Safety: Avoid contact; wear safety spectacles and gloves. In the event of contact with eyes and skin, rinse with water.
Examples of effect: Are harmful to aquatic organisms, toxic or highly toxic, acute or with long-term effects. Safety: To be disposed of as hazardous waste only, do not allow into the environment under any circumstances.
Danger
H315-H318-H411
i causes skin irritation, causes serious eye damage, toxic to aquatic life with long lasting effects
P273 P280 P305+P351+P338 P310
i avoid release to the environment, wear protective gloves/eye protection, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing, immediately call a doctor
Pack Qty.
Pack.
Packaging
Biuret solutions
Density (D) 1,37 g/cm³
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
ADR 9 III
WGK 2
UN-Nr. 3082

For protein quantitation (PCA enhanced Biuret reaction). High tolerance against detergents, salts and alcohols.
Kit contents are adequate for 500/200 preparations in photometer cuvettes or 5000/2000 preparations in microtiter plates.

Colorimetric test for detecting and quantifying soluble proteins. Universally applicable.
Product details
Notes on the product
€201.60 /Pack Qty.

€151.15/Pack Qty.  Campaign price!

excl. VAT. | 1 kit per Pack Qty.

Art. No. 0120.1

In stock
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Product details


Colorimetric test for detecting and quantifying soluble proteins. Universally applicable.

  • Equals the well-known BCA assay
  • Very easy to handle
  • Highly sensitive measurement up to 0.5 µg protein
  • Linear measuring range from 0.5 to 200 µg protein
  • Can be used in 1-ml-scales and in microtiter plates (can be automated)
  • Measurement with standard filters (492 nm wave length) or at 503 nm wave length
  • Accelerated reaction and increased sensitivity in mild heat

The special reagent formula effects a high tolerance against interfering factors such as detergents, alcohol and buffer salts, thus making the kit a universal tool for measuring proteins in almost every solution.


The basic mechanism, a PCA enhanced Biuret reaction, is identical with the one used for the well known BCA protein assay. Its result, however, is a bright blue colour which may be measured with standard filters of approx. 500 nm wave length.



ROTI®Quant universal for biochemistry
Mechanism

ROTI®Quant universal is based on a biuret reaction in combination with a highly specific colorimetric enhancer reaction. The Cu++ ions of reagent 2 are reduced to Cu+ ions in the alkaline reaction media of the test. The colour development of the copper-specific enhancer reaction is directly proportional to the concentration of the protein which is present. The resulting purple-coloured copper complex exhibits a strong light absorption with a maximum of 503 nm, although the wide maximum of the absorption curve also permits highly sensitive and no-loss measurements at 492 nm. The reaction does not form a true end point measurement: the colour develops slowly at room temperature, this, however, can be accelerated in heat. High sensitivity, therefore, can be achieved even after a short time.

0120_Diagramm_AB
Fig. A: ROTI®Quant universal, standard curve with BSA (bovine serum

albumin). Blank-value corrected. Measurement of 100 µl each protein

solution.

Fig. B: Independence of ROTI®Quant-universal-assay of detergents and other additives, examples. Blank-value corrected. Measurement of 100 µl each protein solution.
(With kind permission of Dr. Angelika Böttger, Zoological Institute, Ludwig-Maximilians-University Munich)

The kit contains

500/200 ml ROTI®Quant universal reagent 1 (Art. No. 0118) and 40/16 ml ROTI®Quant universal reagent 2 (Art. No. 0119).
Contents of this Kit may not be bought separately.

0120_Diagramm_D
Fig. D: Independence of ROTI®Quant-universal assay of SDS. Microassay in microtiterplates (60 min, 37 °C).


Technical Information
Detection Biuret reaction/PCA (equals BCA) 
ROTI®Quant universal
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Order No. Pack Qty. Pack. Packaging Price Quantity
0120.1 1 kit plastic 500 assays (cuvettes) €151.15
0120.2 1 kit plastic 200 assays (cuvettes) €71.90
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Notes on the product

Tested for protein gel staining.

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