Technical Data Sheet
ROTI®Quant universal, 500 assays (cuvettes)
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
ADR 9 III
Kit contents are adequate for 500/200 preparations in photometer cuvettes or 5000/2000 preparations in microtiter plates.
€188.30/Pack Qty. Campaign price!
excl. VAT. | 1 kit per Pack Qty.
Art. No. 0120.1
Colorimetric test for detecting and quantifying soluble proteins. Universally applicable.
- Equals the well-known BCA assay
- Very easy to handle
- Highly sensitive measurement up to 0.5 µg protein
- Linear measuring range from 0.5 to 200 µg protein
- Can be used in 1-ml-scales and in microtiter plates (can be automated)
- Measurement with standard filters (492 nm wave length) or at 503 nm wave length
- Accelerated reaction and increased sensitivity in mild heat
The special reagent formula effects a high tolerance against interfering factors such as detergents, alcohol and buffer salts, thus making the kit a universal tool for measuring proteins in almost every solution.
The basic mechanism, a PCA enhanced Biuret reaction, is identical with the one used for the well known BCA protein assay. Its result, however, is a bright blue colour which may be measured with standard filters of approx. 500 nm wave length.
ROTI®Quant universal is based on a biuret reaction in combination with a highly specific colorimetric enhancer reaction. The Cu++ ions of reagent 2 are reduced to Cu+ ions in the alkaline reaction media of the test. The colour development of the copper-specific enhancer reaction is directly proportional to the concentration of the protein which is present.
The resulting purple-coloured copper complex exhibits a strong light absorption with a maximum of 503 nm, although the wide maximum of the absorption curve also permits highly sensitive and no-loss measurements at 492 nm. The reaction does not form a true end point measurement: the colour develops slowly at room temperature, this, however, can be accelerated in heat. High sensitivity, therefore, can be achieved even after a short time.
ROTI®Quant universal for biochemistry
500/200 ml ROTI®Quant universal reagent 1 (Art. No. 0118) and 40/16 ml ROTI®Quant universal reagent 2 (Art. No. 0119).
Contents of this Kit may not be bought separately.
(With kind permission of Dr. Angelika Böttger, Zoological Institute, Ludwig-Maximilians-University Munich)
|Detection||Biuret reaction/PCA (equals BCA)|
- Subtotal: 0.00
|Art. No.||Pack Qty.||Pack.||Packaging||Price||Quantity|
|0120.1||1 kit||plastic||500 assays (cuvettes)||
|0120.2||1 kit||plastic||200 assays (cuvettes)||
No longer available
Delivery date currently unknown
|Tested for protein gel staining.|