Technical Data Sheet
ROTI®Lux OneStep RT-qPCR Master Mix, 1 ml, 1 x 1 ml
The products marked accordingly are shipped refrigerated in special, sustainable cooling boxes with cooling packs or dry ice. Information about our shipping costs can be found here.
Ready-to-use products and reagents
Boiling point (bp) 100 °C
Melting point (mp) 0 °C
Storage temp. -20 °C
Transport temp. cooled
For gene expression analysis - cDNA synthesis and qPCR in one reaction.
The qPCR Master Mixes contain an optimised DNA polymerase that enables ultra-fast runs in less than 20 minutes. Due to the high sensitivity, even the smallest amounts of cDNA (≤1pg) can be detected.
The Master Mixes are also characterised by a particularly high temperature stability, which enables pipetting at room temperature and which means that they remain stable even after several Freeze and Thaw cycles (≥10x).
€157.20/Pack Qty. Campaign price!
excl. VAT. | 1 ml per Pack Qty.
Art. No. 267Y.1
Product details
ROTI®Lux OneStep RT-qPCR Master Mixes are suitable for all common types of probe-based assays, for probe-based genotyping using multiplexing and for the analysis of gene expression in quantitative real-time PCR. All components for cDNA synthesis are already included in the mix, so the isolated RNA can be used directly as starting material.
The qPCR Master Mixes contain an optimised DNA polymerase that enables ultra-fast runs in less than 20 minutes. Due to the high sensitivity, even the smallest amounts of cDNA (≤1pg) can be detected.
The Master Mixes are also characterised by a particularly high temperature stability, which enables pipetting at room temperature and which means that they remain stable even after several Freeze and Thaw cycles (≥10x).
- cDNA synthesis and qPCR in one reaction
- Excellent sensitivity (<0.1 pg RNA per reaction)
- High temperature stability
- Ultra-fast qPCR in <35 min
- Easy handling due to blue staining
- Probe-based qPCR analyses (e. g. TaqMan®)
- Multiplexing
- Gene expression analyses
- Genotyping
ROTI®Lux OneStep RT-qPCR Master Mix 2x conc., ready to use, no ROX
Master Mix without the reference dye ROX (6-Carboxy-X-rhodamine)
- Exicycler™(Bioneer)
- CFX Real-Time PCR Cycler (Biorad)
- RealLine Cycler (Bioron)
- Dtlite & Dtprime Cycler (DNA Technology)
- Mastercycler® (Eppendorf)
- LightCycler® (Roche)
- AriaMx Real-time PCR System (Agilent)
- Q-Cycler (Quantabio)
| Application | Probe-based real-time PCR assays |
| Amplicon ends | 3'dA |
| Basic material | RNA |
| Polymerase | HotStart DNA Polymerase |
| Instructions for use, addition |
The master mix contains all components for reverse transcription and qPCR. Only primer and template must be added separately. 1 ml Master Mix is sufficient for one 96-well plate. |
| Version | low ROX |
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| Art. No. | Pack Qty. | Pack. | Packaging | Price | Quantity | |
|---|---|---|---|---|---|---|
| 267Y.1 | 1 ml | plastic | 1 x 1 ml |
€209.65
€157.20 |
|
|
| 267Y.2 | 5 ml | plastic | 5 x 1 ml |
€940.65
€705.45 |
|
|
| 267Y.3 | 10 ml | plastic | 10 x 1 ml |
€1,725.40
€1,294.00 |
|
|
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