Technical Data Sheet
ROTI®Lux Probe qPCR Master Mix, 1 ml, 1 x 1 ml
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Ready-to-use products and reagents
Boiling point (bp) 100 °C
Storage temp. -20 °C
Transport temp. cooled
WGK 1
For ultra-fast qPCR protocols and probe-based real-time PCR assays. Also perfectly suited for multiplexing assays.
The qPCR Master Mixes contain an optimised DNA polymerase that enables ultra-fast runs in less than 20 minutes. Due to the high sensitivity, even the smallest amounts of cDNA (<1pg) can be detected.
The Master Mixes are also characterised by a particularly high temperature stability, which enables pipetting at room temperature and which means that they remain stable even after several Freeze and Thaw cycles (>10x).
€60.45/Pack Qty. Campaign price!
excl. VAT. | 1 ml per Pack Qty.
Art. No. 25KN.1
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Product details
ROTI®Lux Probe qPCR Master Mixes are suitable for all common types of probe-based assays, for probe-based genotyping using multiplexing and for the analysis of gene expression in quantitative real-time PCR.
The qPCR Master Mixes contain an optimised DNA polymerase that enables ultra-fast runs in less than 20 minutes. Due to the high sensitivity, even the smallest amounts of cDNA (<1pg) can be detected.
The Master Mixes are also characterised by a particularly high temperature stability, which enables pipetting at room temperature and which means that they remain stable even after several Freeze and Thaw cycles (>10x).
- High temperature stability
- Excellent sensitivity (<1 pg cDNA)
- Ultra-fast qPCR in <20 min
- Compatible with all common probe systems (primer probes, hydrolysis probes such as TaqMan®, hybridization probes)
- For all common qPCR cyclers - optionally with or without ROX
- Probe-based qPCR analyses
- Multiplexing
- Gene expression analyses
- Genotyping
ROTI®Lux Probe qPCR Master Mix 2x, ready to use, low ROX
Master mix with low concentration (100 nM) of the reference dye ROX (6-Carboxy-X-rhodamine)
- Quantstudio™ Cycler ( Thermofisher)
- qTower (Analytik Jena)
- Mx3000P® qPCR Systeme (Agilent)
- Rotor-Gene™ (Qiagen)
| Application | Probe-based real-time PCR assays |
| Amplicon ends | 3'dA |
| Basic material | DNA (cDNA / gDNA) |
| Polymerase | HotStart DNA Polymerase |
| Instructions for use, addition |
The master mix contains a modified HotStart DNA polymerase, dNTPs, MgCl2 and the reaction buffer. Primer and template must be added separately. 1 ml Master Mix is sufficient for one 96-well plate. |
| Version | low ROX |
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| Art. No. | Pack Qty. | Pack. | Packaging | Price | Quantity | |
|---|---|---|---|---|---|---|
| 25KN.1 | 1 ml | plastic | 1 x 1 ml |
€80.65
€60.45 |
|
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| 25KN.2 | 5 ml | plastic | 5 x 1 ml |
€376.25
€282.15 |
|
|
| 25KN.3 | 10 ml | plastic | 10 x 1 ml |
€698.75
€524.05 |
|
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